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PI 3 Kinase regulatory subunit 4 (PIK3R4) (NM_014602) Human Tagged ORF Clone
CAT＃: RC210308
PIK3R4 (Myc-DDK-tagged)-Human phosphoinositide-3-kinase, regulatory subunit 4 (PIK3R4)
ORF Plasmid: tGFP
"NM_014602" in other vectors (6)
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CNY 9,296.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | p150; VPS15 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210308 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAAATCAGCTTGCTGGCATTGCTCCCTCCCAGATCCTTTCTGTAGAGAGTTATTTTTCAGATATTC ATGACTTTGAATATGATAAAAGCCTGGGGAGTACTCGGTTTTTTAAAGTTGCTCGAGCCAAGCACCGAGA AGGCCTGGTCGTTGTGAAGGTTTTTGCAATTCAGGATCCCACATTGCCTTTAACCAGCTATAAACAAGAG CTGGAGGAACTGAAAATCAGGCTTAATTCTGCACAGAATTGTCTACCTTTCCAGAAAGCATCAGAAAAAG CATCTGAGAAAGCAGCTATGCTCTTTAGGCAGTATGTGCGAGACAATCTCTATGATCGCATCAGTACCCG TCCATTCTTGAATAACATTGAGAAGCGCTGGATTGCTTTCCAGATCCTGACAGCTGTGGACCAAGCACAC AAATCTGGAGTTCGTCATGGGGACATCAAGACTGAGAATGTGATGGTCACCAGTTGGAATTGGGTTCTTC TAACTGATTTTGCCAGTTTTAAGCCCACTTATCTTCCAGAAGACAACCCGGCAGATTTCAATTATTTCTT TGACACATCACGGAGGAGAACTTGCTATATTGCTCCTGAACGTTTTGTTGATGGTGGGATGTTTGCCACT GAGTTAGAATATATGAGAGATCCTTCAACTCCGCTTGTAGACTTAAATAGCAATCAGAGAACAAGAGGAG AGTTGAAGAGAGCAATGGACATCTTTTCAGCAGGTTGTGTGATAGCTGAGCTTTTTACAGAAGGTGTACC ATTATTTGATCTCTCTCAACTTTTGGCTTATAGAAATGGACATTTTTTCCCTGAACAAGTGCTAAATAAA ATTGAAGATCACAGTATCAGAGAATTGGTAACTCAGATGATTCACCGTGAGCCAGATAAACGTTTAGAGG CAGAAGATTACTTAAAACAGCAGCGTGGCAATGCCTTTCCTGAAATATTTTACACTTTTCTTCAGCCCTA CATGGCCCAGTTTGCCAAGGAAACGTTTCTTTCTGCAGATGAGCGTATTCTGGTTATACGGAAGGATTTG GGCAACATTATTCACAATCTCTGTGGACATGATCTGCCAGAAAAAGCCGAAGGAGAGCCTAAGGAAAATG GGCTGGTTATCTTGGTATCTGTTATAACATCCTGCCTACAGACCCTTAAATACTGTGATTCCAAACTAGC TGCTTTGGAACTGATTCTTCATTTGGCTCCAAGATTAAGTGTTGAAATCCTTTTGGATCGTATTACTCCA TATCTTTTGCATTTCAGCAATGACTCTGTTCCTAGGGTGAGGGCTGAAGCCTTGAGGACGTTGACCAAAG TTCTTGCTCTCGTCAAAGAGGTTCCTCGTAATGATATCAATATTTATCCGGAATACATTCTGCCAGGCAT AGCCCACTTAGCCCAAGATGATGCTACTATCGTTAGACTAGCCTATGCTGAAAACATAGCTCTGCTGGCA GAAACAGCTCTGAGATTCCTGGAATTAGTACAGTTAAAAAATCTTAATATGGAAAATGACCCCAATAATG AAGAAATAGATGAGGTTACACATCCAAATGGAAATTATGACACAGAGCTCCAAGCCTTACATGAAATGGT CCAGCAGAAAGTTGTTACTTTGCTAAGTGACCCTGAAAATATTGTAAAACAAACCTTGATGGAAAATGGA ATAACACGGCTGTGTGTATTCTTTGGACGTCAGAAAGCCAACGATGTTTTGTTGTCCCACATGATTACTT TCCTAAATGATAAGAATGATTGGCATCTACGTGGAGCATTTTTTGATAGTATAGTTGGTGTTGCTGCCTA TGTTGGCTGGCAAAGCTCCTCAATTCTCAAGCCTCTGCTGCAACAAGGTCTTAGTGATGCTGAGGAATTT GTCATTGTGAAAGCTCTTTATGCCCTTACTTGTATGTGCCAGTTAGGACTGCTACAAAAACCCCATGTTT ACGAATTTGCCAGTGATATTGCCCCCTTCCTGTGTCATCCCAATTTATGGATACGTTATGGTGCCGTGGG ATTTATCACAGTGGTAGCTCGTCAAATAAGTACAGCTGATGTCTACTGTAAACTGATGCCTTATCTTGAC CCATATATTACCCAACCAATAATACAGATTGAAAGAAAACTTGTTCTGCTCAGTGTTTTAAAGGAACCAG TAAGTCGTTCTATATTTGATTATGCTTTGAGGTCTAAAGATATTACTAGCTTGTTCAGACATCTTCACAT GCGTCAGAAGAAACGAAATGGTTCTCTTCCCGACTGCCCTCCGCCAGAGGATCCTGCCATAGCACAGCTT CTGAAGAAGTTGCTCTCACAGGGAATGACAGAGGAAGAGGAAGACAAACTTCTGGCACTGAAAGACTTCA TGATGAAATCTAATAAAGCAAAGGCCAATATAGTGGACCAGAGCCATCTTCATGATAGTAGTCAGAAAGG TGTAATTGACTTGGCAGCTTTAGGCATAACTGGGAGACAAGTTGATCTTGTTAAAACCAAACAAGAACCA GATGACAAACGGGCCAGAAAACATGTAAAACAAGACTCAAATGTAAATGAAGAATGGAAAAGCATGTTTG GGTCACTGGACCCACCAAACATGCCACAGGCCCTACCTAAAGGGAGTGATCAGGAGGTGATTCAGACTGG GAAACCTCCTCGTTCCGAGTCCTCTGCTGGCATTTGTGTCCCTTTGTCAACTTCTTCACAGGTTCCAGAA GTGACAACTGTCCAAAATAAAAAACCAGTAATACCGGTTTTAAGTAGTACAATCTTACCATCCACCTATC AGATTCGAATTACAACTTGTAAAACTGAACTTCAGCAACTCATCCAGCAAAAGCGGGAGCAGTGCAATGC TGAGAGAATAGCTAAGCAGATGATGGAAAATGCTGAATGGGAGAGTAAACCACCACCACCTGGATGGCGT CCTAAAGGGCTGTTAGTTGCCCATCTTCATGAGCATAAATCTGCTGTGAATCGAATTAGAGTCTCTGATG AACACTCACTTTTTGCAACATGTTCAAATGATGGCACAGTGAAAATCTGGAACAGTCAAAAGATGGAGGG GAAGACCACCACTACCAGATCTATTCTTACATACAGCCGAATTGGAGGACGAGTCAAGACGCTCACATTC TGCCAAGGCTCCCACTATTTAGCCATAGCATCTGATAATGGTGCTGTCCAGCTTCTTGGAATTGAGGCTT CTAAGCTGCCCAAGTCTCCTAAAATCCATCCTCTACAAAGCAGAATTCTAGATCAGAAGGAGGACGGTTG TGTTGTGGATATGCATCACTTCAACTCTGGAGCACAGTCTGTTCTTGCCTATGCCACTGTGAATGGCTCT CTGGTTGGCTGGGACCTTAGGTCTTCAAGCAATGCGTGGACTTTAAAGCATGATTTAAAGTCGGGCCTCA TCACTTCCTTTGCTGTGGACATCCACCAATGCTGGCTCTGCATTGGTACAAGCAGTGGTACCATGGCTTG TTGGGACATGAGGTTCCAGTTGCCAATTTCAAGTCACTGTCATCCTTCCAGGGCTCGAATCAGACGCCTC TCAATGCACCCTCTGTATCAGTCCTGGGTGATTGCAGCTGTTCAGGGCAACAACGAAGTGTCCATGTGGG ACATGGAGACTGGTGACAGAAGATTTACTCTCTGGGCCAGCAGTGCACCACCACTTTCTGAATTACAGCC TTCTCCTCATAGCGTCCATGGTATCTACTGTAGTCCTGCAGATGGAAATCCTATCCTACTAACAGCTGGC TCAGATATGAAAATAAGGTTTTGGGACTTGGCTTACCCAGAAAGGTCCTATGTTGTTGCAGGAAGTACTA GTTCCCCATCTGTGTCCTACTACAGGAAAATAATTGAAGGCACTGAAGTTGTCCAGGAAATTCAGAATAA GCAGAAAGTAGGACCAAGTGATGACACCCCTCGAAGGGGCCCAGAGTCCCTGCCCGTGGGACATCATGAC ATCATCACTGATGTCGCCACATTCCAGACCACACAGGGCTTCATCGTAACTGCTTCTAGAGATGGGATTG TGAAGGTGTGGAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210308 protein sequence
Red=Cloning site Green=Tags(s) MGNQLAGIAPSQILSVESYFSDIHDFEYDKSLGSTRFFKVARAKHREGLVVVKVFAIQDPTLPLTSYKQE LEELKIRLNSAQNCLPFQKASEKASEKAAMLFRQYVRDNLYDRISTRPFLNNIEKRWIAFQILTAVDQAH KSGVRHGDIKTENVMVTSWNWVLLTDFASFKPTYLPEDNPADFNYFFDTSRRRTCYIAPERFVDGGMFAT ELEYMRDPSTPLVDLNSNQRTRGELKRAMDIFSAGCVIAELFTEGVPLFDLSQLLAYRNGHFFPEQVLNK IEDHSIRELVTQMIHREPDKRLEAEDYLKQQRGNAFPEIFYTFLQPYMAQFAKETFLSADERILVIRKDL GNIIHNLCGHDLPEKAEGEPKENGLVILVSVITSCLQTLKYCDSKLAALELILHLAPRLSVEILLDRITP YLLHFSNDSVPRVRAEALRTLTKVLALVKEVPRNDINIYPEYILPGIAHLAQDDATIVRLAYAENIALLA ETALRFLELVQLKNLNMENDPNNEEIDEVTHPNGNYDTELQALHEMVQQKVVTLLSDPENIVKQTLMENG ITRLCVFFGRQKANDVLLSHMITFLNDKNDWHLRGAFFDSIVGVAAYVGWQSSSILKPLLQQGLSDAEEF VIVKALYALTCMCQLGLLQKPHVYEFASDIAPFLCHPNLWIRYGAVGFITVVARQISTADVYCKLMPYLD PYITQPIIQIERKLVLLSVLKEPVSRSIFDYALRSKDITSLFRHLHMRQKKRNGSLPDCPPPEDPAIAQL LKKLLSQGMTEEEEDKLLALKDFMMKSNKAKANIVDQSHLHDSSQKGVIDLAALGITGRQVDLVKTKQEP DDKRARKHVKQDSNVNEEWKSMFGSLDPPNMPQALPKGSDQEVIQTGKPPRSESSAGICVPLSTSSQVPE VTTVQNKKPVIPVLSSTILPSTYQIRITTCKTELQQLIQQKREQCNAERIAKQMMENAEWESKPPPPGWR PKGLLVAHLHEHKSAVNRIRVSDEHSLFATCSNDGTVKIWNSQKMEGKTTTTRSILTYSRIGGRVKTLTF CQGSHYLAIASDNGAVQLLGIEASKLPKSPKIHPLQSRILDQKEDGCVVDMHHFNSGAQSVLAYATVNGS LVGWDLRSSSNAWTLKHDLKSGLITSFAVDIHQCWLCIGTSSGTMACWDMRFQLPISSHCHPSRARIRRL SMHPLYQSWVIAAVQGNNEVSMWDMETGDRRFTLWASSAPPLSELQPSPHSVHGIYCSPADGNPILLTAG SDMKIRFWDLAYPERSYVVAGSTSSPSVSYYRKIIEGTEVVQEIQNKQKVGPSDDTPRRGPESLPVGHHD IITDVATFQTTQGFIVTASRDGIVKVWK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014602 |
| ORF Size | 4074 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014602.3 |
| RefSeq Size | 5078 bp |
| RefSeq ORF | 4077 bp |
| Locus ID | 30849 |
| UniProt ID | Q99570 |
| Domains | pkinase, WD40, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Regulation of autophagy |
| MW | 153.1 kDa |
| Gene Summary | Regulatory subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210308L1 | Lenti ORF clone of Human phosphoinositide-3-kinase, regulatory subunit 4 (PIK3R4), Myc-DDK-tagged |
CNY 11,696.00 |
|
| RC210308L2 | Lenti ORF clone of Human phosphoinositide-3-kinase, regulatory subunit 4 (PIK3R4), mGFP tagged |
CNY 15,490.00 |
|
| RC210308L3 | Lenti ORF clone of Human phosphoinositide-3-kinase, regulatory subunit 4 (PIK3R4), Myc-DDK-tagged |
CNY 15,490.00 |
|
| RC210308L4 | Lenti ORF clone of Human phosphoinositide-3-kinase, regulatory subunit 4 (PIK3R4), mGFP tagged |
CNY 15,490.00 |
|
| RG210308 | PIK3R4 (tGFP-tagged) - Human phosphoinositide-3-kinase, regulatory subunit 4 (PIK3R4) |
CNY 10,896.00 |
|
| SC100382 | PIK3R4 (untagged)-Human phosphoinositide-3-kinase, regulatory subunit 4 (PIK3R4) |
CNY 9,296.00 |
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