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LMO1 (NM_002315) Human Tagged ORF Clone
CAT＃: RC210233
LMO1 (Myc-DDK-tagged)-Human LIM domain only 1 (rhombotin 1) (LMO1)
ORF Plasmid: tGFP
"NM_002315" in other vectors (4)
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CNY 1,200.00
CNY 3,705.00
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CNY 300.00
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| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | RBTN1; RHOM1; TTG1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210233 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGTGCTGGACAAGGAGGACGGCGTGCCGATGCTCTCCGTCCAGCCCAAAGGGAAGCAGAAGGGCT GTGCGGGCTGTAACCGCAAGATCAAGGACCGCTATCTGCTGAAGGCATTGGACAAGTACTGGCACGAAGA CTGCCTCAAGTGTGCCTGCTGTGACTGCCGCCTGGGCGAGGTGGGCTCCACCCTCTACACCAAGGCCAAC CTCATCCTGTGCCGACGCGACTACCTGAGGCTCTTTGGCACCACAGGGAACTGTGCTGCTTGCAGCAAGC TGATCCCAGCCTTCGAGATGGTGATGCGGGCCCGGGACAACGTGTATCACCTCGACTGCTTCGCCTGCCA GCTCTGCAACCAGAGATTTTGTGTGGGAGACAAATTCTTCCTGAAGAACAACATGATCTTGTGTCAGATG GACTATGAGGAAGGGCAGCTCAATGGCACCTTTGAATCCCAAGTTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210233 protein sequence
Red=Cloning site Green=Tags(s) MMVLDKEDGVPMLSVQPKGKQKGCAGCNRKIKDRYLLKALDKYWHEDCLKCACCDCRLGEVGSTLYTKAN LILCRRDYLRLFGTTGNCAACSKLIPAFEMVMRARDNVYHLDCFACQLCNQRFCVGDKFFLKNNMILCQM DYEEGQLNGTFESQVQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002315 |
| ORF Size | 468 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002315.3 |
| RefSeq Size | 1315 bp |
| RefSeq ORF | 471 bp |
| Locus ID | 4004 |
| UniProt ID | P25800 |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 17.8 kDa |
| Gene Summary | This locus encodes a transcriptional regulator that contains two cysteine-rich LIM domains but lacks a DNA-binding domain. LIM domains may play a role in protein interactions; thus the encoded protein may regulate transcription by competitively binding to specific DNA-binding transcription factors. Alterations at this locus have been associated with acute lymphoblastic T-cell leukemia. Chromosomal rearrangements have been observed between this locus and at least two loci, the delta subunit of the T-cell antigen receptor gene and the LIM domain binding 1 gene. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210233L3 | Lenti ORF clone of Human LIM domain only 1 (rhombotin 1) (LMO1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210233L4 | Lenti ORF clone of Human LIM domain only 1 (rhombotin 1) (LMO1), mGFP tagged |
CNY 5,890.00 |
|
| RG210233 | LMO1 (tGFP-tagged) - Human LIM domain only 1 (rhombotin 1) (LMO1) |
CNY 2,800.00 |
|
| SC303178 | LMO1 (untagged)-Human LIM domain only 1 (rhombotin 1) (LMO1) |
CNY 3,990.00 |
