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MYF5 (NM_005593) Human Tagged ORF Clone
CAT＃: RC210156
MYF5 (Myc-DDK-tagged)-Human myogenic factor 5 (MYF5)
ORF Plasmid: tGFP
"NM_005593" in other vectors (6)
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CNY 2,400.00
CNY 3,705.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | bHLHc2; EORVA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210156 representing NM_005593
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGTGATGGATGGCTGCCAGTTCTCACCTTCTGAGTACTTCTACGACGGCTCCTGCATACCGTCCC CCGAGGGTGAATTTGGGGACGAGTTTGTGCCGCGAGTGGCTGCCTTCGGAGCGCACAAAGCAGAGCTGCA GGGCTCAGATGAGGACGAGCACGTGCGAGCGCCTACCGGCCACCACCAGGCTGGTCACTGCCTCATGTGG GCCTGCAAAGCCTGCAAGAGGAAGTCCACCACCATGGATCGGCGGAAGGCAGCCACTATGCGCGAGCGGA GGCGCCTGAAGAAGGTCAACCAGGCTTTCGAAACCCTCAAGAGGTGTACCACGACCAACCCCAACCAGAG GCTGCCCAAGGTGGAGATCCTCAGGAATGCCATCCGCTACATCGAGAGCCTGCAGGAGTTGCTGAGAGAG CAGGTGGAGAACTACTATAGCCTGCCGGGACAGAGCTGCTCGGAGCCCACCAGCCCCACCTCCAACTGCT CTGATGGCATGCCCGAATGTAACAGTCCTGTCTGGTCCAGAAAGAGCAGTACTTTTGACAGCATCTACTG TCCTGATGTATCAAATGTATATGCCACAGATAAAAACTCCTTATCCAGCTTGGATTGCTTATCCAACATA GTGGACCGGATCACCTCCTCAGAGCAACCTGGGTTGCCTCTCCAGGATCTGGCTTCTCTCTCTCCAGTTG CCAGCACCGATTCACAGCCTGCAACTCCAGGGGCTTCTAGTTCCAGGCTTATCTATCATGTGCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210156 representing NM_005593
Red=Cloning site Green=Tags(s) MDVMDGCQFSPSEYFYDGSCIPSPEGEFGDEFVPRVAAFGAHKAELQGSDEDEHVRAPTGHHQAGHCLMW ACKACKRKSTTMDRRKAATMRERRRLKKVNQAFETLKRCTTTNPNQRLPKVEILRNAIRYIESLQELLRE QVENYYSLPGQSCSEPTSPTSNCSDGMPECNSPVWSRKSSTFDSIYCPDVSNVYATDKNSLSSLDCLSNI VDRITSSEQPGLPLQDLASLSPVASTDSQPRTPGASSSRLIYHVL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005593 |
| ORF Size | 765 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005593.3 |
| RefSeq Size | 1427 bp |
| RefSeq ORF | 768 bp |
| Locus ID | 4617 |
| UniProt ID | P13349 |
| Domains | HLH, Basic |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 28.1 kDa |
| Gene Summary | Transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation (PubMed:29887215). Together with MYOG and MYOD1, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Recessive MYF5 Mutations Cause External Ophthalmoplegia, Rib, and Vertebral Anomalies
,Di Gioia, SA;Shaaban, S;Tüysüz, B;Elcioglu, NH;Chan, WM;Robson, CD;Ecklund, K;Gilette, NM;Hamzaoglu, A;Tayfun, GA;Traboulsi, EI;Engle, EC;,
Am. J. Hum. Genet.
,PubMed ID 29887215
[MYF5]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210156L1 | Lenti ORF clone of Human myogenic factor 5 (MYF5), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC210156L2 | Lenti ORF clone of Human myogenic factor 5 (MYF5), mGFP tagged |
CNY 5,890.00 |
|
| RC210156L3 | Lenti ORF clone of Human myogenic factor 5 (MYF5), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC210156L4 | Lenti ORF clone of Human myogenic factor 5 (MYF5), mGFP tagged |
CNY 4,800.00 |
|
| RG210156 | MYF5 (tGFP-tagged) - Human myogenic factor 5 (MYF5) |
CNY 4,370.00 |
|
| SC311244 | MYF5 (untagged)-Human myogenic factor 5 (MYF5) |
CNY 2,400.00 |
