CER1 (NM_005454) Human Tagged ORF Clone
CAT#: RC210111
CER1 (Myc-DDK-tagged) - Human cerberus 1, DAN family BMP antagonist (CER1)
ORF Plasmid: tGFP
"NM_005454" in other vectors (4)
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CNY 2,400.00
CNY 3,990.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | DAND4 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC210111 representing NM_005454.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCATCTCCTCTTATTTCAGCTGCTGGTACTCCTGCCTCTAGGAAAGACCACACGGCACCAGGATGGC CGCCAGAATCAGAGTTCTCTTTCCCCCGTACTCCTGCCAAGGAATCAAAGAGAGCTTCCCACAGGCAAC CATGAGGAAGCTGAGGAGAAGCCAGATCTGTTTGTCGCAGTGCCACACCTTGTAGGCACCAGCCCTGCA GGGGAAGGCCAGAGGCAGAGAGAGAAGATGCTGTCCAGATTTGGCAGGTTCTGGAAGAAGCCTGAGAGA GAAATGCATCCATCCAGGGACTCAGATAGTGAGCCCTTCCCACCTGGGACCCAGTCCCTCATCCAGCCG ATAGATGGAATGAAAATGGAGAAATCTCCTCTTCGGGAAGAAGCCAAGAAATTCTGGCACCACTTCATG TTCAGAAAAACTCCGGCTTCTCAGGGGGTCATCTTGCCCATCAAAAGCCATGAAGTACATTGGGAGACC TGCAGGACAGTGCCCTTCAGCCAGACTATAACCCACGAAGGCTGTGAGAAAGTAGTTGTTCAGAACAAC CTTTGCTTTGGGAAATGCGGGTCTGTTCATTTTCCTGGAGCCGCGCAGCACTCCCATACCTCCTGCTCT CACTGTTTGCCTGCCAAGTTCACCACGATGCACTTGCCAGTGAACTGCACTGAACTTTCCTCCGTGATC AAGGTGGTGATGCTGGTGGAGGAGTGCCAGTGCAAGGTGAAGACGGAGCATGAAGATGGACACATCCTA CATGCTGGCTCCCAGGATTCCTTTATCCCAGGAGTTTCAGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC >Peptide sequence encoded by RC210111
Blue=ORF Red=Cloning site Green=Tag(s) MHLLLFQLLVLLPLGKTTRHQDGRQNQSSLSPVLLPRNQRELPTGNHEEAEEKPDLFVAVPHLVGTSPA GEGQRQREKMLSRFGRFWKKPEREMHPSRDSDSEPFPPGTQSLIQPIDGMKMEKSPLREEAKKFWHHFM FRKTPASQGVILPIKSHEVHWETCRTVPFSQTITHEGCEKVVVQNNLCFGKCGSVHFPGAAQHSHTSCS HCLPAKFTTMHLPVNCTELSSVIKVVMLVEECQCKVKTEHEDGHILHAGSQDSFIPGVSA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_005454 |
ORF Size | 801 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq Size | 1206 bp |
RefSeq ORF | 804 bp |
Locus ID | 9350 |
UniProt ID | O95813 |
Protein Families | Secreted Protein |
Protein Pathways | Wnt signaling pathway |
MW | 30.1 kDa |
Gene Summary | This gene encodes a cytokine member of the cysteine knot superfamily, characterized by nine conserved cysteines and a cysteine knot region. The cerberus-related cytokines, together with Dan and DRM/Gremlin, represent a group of bone morphogenetic protein (BMP) antagonists that can bind directly to BMPs and inhibit their activity. [provided by RefSeq, Jul 2008] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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A selective inhibitor of ceramide synthase 1 reveals a novel role in fat metabolism
,Turner, N;Lim, XY;Toop, HD;Osborne, B;Brandon, AE;Taylor, EN;Fiveash, CE;Govindaraju, H;Teo, JD;McEwen, HP;Couttas, TA;Butler, SM;Das, A;Kowalski, GM;Bruce, CR;Hoehn, KL;Fath, T;Schmitz-Peiffer, C;Cooney, GJ;Montgomery, MK;Morris, JC;Don, AS;,
Nat Commun
,PubMed ID 30131496
[CER1]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC210111L3 | Lenti ORF clone of Human cerberus 1, DAN family BMP antagonist (CER1), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC210111L4 | Lenti ORF clone of Human cerberus 1, DAN family BMP antagonist (CER1), mGFP tagged |
CNY 5,890.00 |
|
RG210111 | CER1 (tGFP-tagged) - Human cerberus 1, DAN family BMP antagonist (CER1), mGFP tagged |
CNY 4,370.00 |
|
SC303647 | CER1 (untagged) - Human cerberus 1, DAN family BMP antagonist (CER1) |
CNY 2,400.00 |