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Cezanne (OTUD7B) (NM_020205) Human Tagged ORF Clone
CAT＃: RC209932
OTUD7B (Myc-DDK-tagged)-Human OTU domain containing 7B (OTUD7B)
ORF Plasmid: tGFP
"NM_020205" in other vectors (6)
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CNY 6,176.00
CNY 7,130.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CEZANNE; ZA20D1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209932 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCCTGGACATGGATGCTGTTCTGTCAGATTTTGTCCGTTCCACAGGAGCAGAGCCAGGGCTAGCGC GAGATCTCCTAGAAGGAAAGAATTGGGATGTGAATGCCGCCCTCAGTGATTTTGAACAGCTACGTCAAGT CCATGCTGGAAACCTACCCCCATCCTTTAGTGAGGGGAGTGGTGGCTCCAGGACCCCTGAAAAAGGGTTT TCTGACAGAGAGCCTACTCGCCCTCCCCGACCCATCCTCCAGCGGCAGGATGACATCGTTCAAGAAAAAC GCCTGTCTAGGGGCATCTCCCACGCCAGCTCCAGCATTGTTTCCCTGGCCCGGTCCCATGTCTCCTCCAA TGGTGGGGGTGGGGGGAGCAATGAGCACCCCCTGGAAATGCCCATCTGTGCCTTCCAGCTTCCAGATCTC ACTGTATACAATGAAGACTTCCGCAGCTTCATAGAGAGAGACCTCATTGAGCAGTCCATGCTGGTTGCCT TGGAACAGGCAGGGCGTTTGAACTGGTGGGTGAGTGTGGATCCCACCTCTCAGAGGCTGCTTCCTTTGGC AACTACTGGAGATGGGAACTGCCTCCTGCATGCAGCCTCCCTTGGAATGTGGGGTTTCCATGATCGGGAC TTGATGCTGCGGAAAGCTTTGTATGCACTGATGGAGAAGGGAGTTGAGAAGGAAGCGTTGAAAAGGCGCT GGAGGTGGCAGCAGACACAGCAGAATAAAGAGTCAGGGCTGGTATACACAGAAGATGAATGGCAGAAGGA GTGGAATGAACTGATCAAGCTTGCCTCAAGTGAACCCCGAATGCATCTAGGTACCAATGGAGCCAACTGT GGTGGGGTGGAGAGTTCTGAGGAGCCTGTATATGAGAGCCTTGAAGAGTTTCACGTCTTTGTCCTTGCTC ATGTGCTTAGGAGGCCCATAGTCGTCGTGGCAGACACCATGCTGAGGGACTCCGGAGGGGAAGCATTTGC CCCTATTCCCTTTGGAGGAATCTATCTGCCTTTGGAGGTCCCAGCCAGCCAGTGTCACCGCTCCCCTCTG GTGCTCGCCTATGATCAGGCCCACTTTTCTGCACTCGTGTCCATGGAGCAGAAGGAGAATACCAAGGAAC AAGCTGTGATCCCACTTACAGATTCAGAGTATAAGCTGCTGCCCTTGCACTTTGCTGTGGACCCTGGAAA GGGCTGGGAGTGGGGCAAAGATGATAGTGACAATGTCCGATTGGCCAGTGTAATTCTGTCCCTAGAGGTC AAATTGCATCTGCTGCATAGCTACATGAATGTGAAGTGGATCCCACTGTCCTCTGATGCACAGGCTCCTC TGGCCCAGCCTGAGTCCCCCACCGCCTCAGCTGGAGATGAGCCCCGGTCCACTCCTGAGTCTGGAGACTC AGACAAGGAGTCAGTTGGCAGCAGTTCCACCAGCAACGAGGGCGGCCGGCGGAAGGAGAAGTCAAAGCGA GATCGGGAGAAGGACAAGAAGAGAGCAGATTCTGTGGCTAACAAACTGGGCAGCTTTGGCAAAACCTTGG GCAGCAAGCTCAAGAAGAACATGGGGGGCCTGATGCACAGCAAGGGTTCAAAGCCTGGAGGGGTGGGGAC AGGGTTGGGAGGAAGCAGCGGCACTGAGACACTGGAGAAGAAGAAGAAAAACTCACTGAAGAGCTGGAAG GGTGGCAAGGAGGAGGCAGCTGGGGATGGGCCTGTGTCTGAGAAGCCCCCAGCTGAGTCTGTTGGTAACG GAGGGAGCAAGTATAGCCAGGAGGTGATGCAGAGCCTGAGCATTCTGAGGACTGCCATGCAAGGGGAGGG GAAGTTTATTTTTGTTGGAACCCTGAAGATGGGTCACCGTCACCAGTATCAGGAGGAAATGATCCAGCGC TACCTTTCTGATGCTGAGGAGAGATTCCTGGCAGAACAGAAGCAGAAGGAGGCAGAGAGGAAGATCATGA ATGGAGGAATAGGGGGTGGCCCTCCTCCAGCCAAAAAGCCAGAGCCAGATGCTAGGGAAGAGCAGCCGAC CGGTCCCCCAGCAGAGTCCAGGGCAATGGCATTTTCCACTGGCTACCCTGGGGACTTTACTATCCCTCGG CCGTCTGGGGGCGGAGTCCACTGCCAGGAACCCCGGAGGCAGTTGGCAGGGGGTCCATGTGTCGGGGGCC TACCACCATATGCCACCTTCCCCAGACAGTGCCCTCCTGGGCGACCCTACCCCCACCAGGACAGCATCCC TTCTCTGGAGCCAGGCAGCCACTCTAAGGATGGACTTCACAGGGGTGCCTTGTTACCACCCCCCTACCGA GTGGCTGATTCCTATAGCAATGGCTACAGAGAGCCCCCTGAGCCAGATGGATGGGCTGGAGGTCTCCGGG GCCTTCCCCCAACTCAGACCAAATGCAAACAACCGAACTGCAGCTTCTATGGACACCCTGAGACAAACAA CTTCTGTTCCTGTTGTTACAGGGAAGAACTGAGGAGGAGGGAGCGGGAACCGGATGGGGAGCTCCTGGTG CACAGGTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209932 protein sequence
Red=Cloning site Green=Tags(s) MTLDMDAVLSDFVRSTGAEPGLARDLLEGKNWDVNAALSDFEQLRQVHAGNLPPSFSEGSGGSRTPEKGF SDREPTRPPRPILQRQDDIVQEKRLSRGISHASSSIVSLARSHVSSNGGGGGSNEHPLEMPICAFQLPDL TVYNEDFRSFIERDLIEQSMLVALEQAGRLNWWVSVDPTSQRLLPLATTGDGNCLLHAASLGMWGFHDRD LMLRKALYALMEKGVEKEALKRRWRWQQTQQNKESGLVYTEDEWQKEWNELIKLASSEPRMHLGTNGANC GGVESSEEPVYESLEEFHVFVLAHVLRRPIVVVADTMLRDSGGEAFAPIPFGGIYLPLEVPASQCHRSPL VLAYDQAHFSALVSMEQKENTKEQAVIPLTDSEYKLLPLHFAVDPGKGWEWGKDDSDNVRLASVILSLEV KLHLLHSYMNVKWIPLSSDAQAPLAQPESPTASAGDEPRSTPESGDSDKESVGSSSTSNEGGRRKEKSKR DREKDKKRADSVANKLGSFGKTLGSKLKKNMGGLMHSKGSKPGGVGTGLGGSSGTETLEKKKKNSLKSWK GGKEEAAGDGPVSEKPPAESVGNGGSKYSQEVMQSLSILRTAMQGEGKFIFVGTLKMGHRHQYQEEMIQR YLSDAEERFLAEQKQKEAERKIMNGGIGGGPPPAKKPEPDAREEQPTGPPAESRAMAFSTGYPGDFTIPR PSGGGVHCQEPRRQLAGGPCVGGLPPYATFPRQCPPGRPYPHQDSIPSLEPGSHSKDGLHRGALLPPPYR VADSYSNGYREPPEPDGWAGGLRGLPPTQTKCKQPNCSFYGHPETNNFCSCCYREELRRREREPDGELLV HRF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020205 |
| ORF Size | 2529 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020205.4 |
| RefSeq Size | 6415 bp |
| RefSeq ORF | 2532 bp |
| Locus ID | 56957 |
| UniProt ID | Q6GQQ9 |
| MW | 92.5 kDa |
| Gene Summary | Negative regulator of the non-canonical NF-kappa-B pathway that acts by mediating deubiquitination of TRAF3, an inhibitor of the NF-kappa-B pathway, thereby acting as a negative regulator of B-cell responses. In response to non-canonical NF-kappa-B stimuli, deubiquitinates 'Lys-48'-linked polyubiquitin chains of TRAF3, preventing TRAF3 proteolysis and over-activation of non-canonical NF-kappa-B. Negatively regulates mucosal immunity against infections (By similarity). Deubiquitinates ZAP70, and thereby regulates T cell receptor (TCR) signaling that leads to the activation of NF-kappa-B (PubMed:26903241). Plays a role in T cell homeostasis and is required for normal T cell responses, including production of IFNG and IL2 (By similarity). Mediates deubiquitination of EGFR (PubMed:22179831). Has deubiquitinating activity toward 'Lys-11', 'Lys-48' and 'Lys-63'-linked polyubiquitin chains (PubMed:27732584). Has a much higher catalytic rate with 'Lys-11'-linked polyubiquitin chains (in vitro); however the physiological significance of these data are unsure (PubMed:27732584). Hydrolyzes both linear and branched forms of polyubiquitin.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209932L1 | Lenti ORF clone of Human OTU domain containing 7B (OTUD7B), Myc-DDK-tagged |
CNY 8,576.00 |
|
| RC209932L2 | Lenti ORF clone of Human OTU domain containing 7B (OTUD7B), mGFP tagged |
CNY 9,030.00 |
|
| RC209932L3 | Lenti ORF clone of Human OTU domain containing 7B (OTUD7B), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC209932L4 | Lenti ORF clone of Human OTU domain containing 7B (OTUD7B), mGFP tagged |
CNY 9,030.00 |
|
| RG209932 | OTUD7B (tGFP-tagged) - Human OTU domain containing 7B (OTUD7B) |
CNY 7,776.00 |
|
| SC304721 | OTUD7B (untagged)-Human OTU domain containing 7B (OTUD7B) |
CNY 7,220.00 |
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