ZNF770 (NM_014106) Human Tagged ORF Clone

CAT#: RC209873

ZNF770 (Myc-DDK-tagged)-Human zinc finger protein 770 (ZNF770)

ORF Plasmid: DDK tGFP



  "NM_014106" in other vectors (4)

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CNY 5,064.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms PRO1914
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC209873 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGGCTGAAAACAATTTAAAAATGCTAAAGATTCAACAGTGTGTGGTAGCCAACAAACTACCTAGAA
ACAGGCCATATGTTTGCAATATTTGTTTTAAGCACTTTGAAACACCATCAAAATTAGCTAGGCACTATCT
CATTCATACTGGTCAAAAGCCATTTGAATGTGATGTGTGTCATAAAACCTTTAGACAACTAGTTCATCTG
GAGAGGCATCAACTAACTCATAGTCTGCCTTTTAAATGTAGTATTTGTCAGCGTCACTTTAAAAATCTGA
AGACATTTGTGAAGCACCAACAACTTCACAATGAAACCTATCAGAATAATGTTAAACAGGTCAGAAGATT
GCTGGAGGCCAAGCAAGAAAAGTCAATGTATGGAGTGTATAATACTTTTACCACAGAGGAAAGATGGGCA
TTACACCCGTGCTCTAAGTCTGATCCCATGTATAGCATGAAAAGAAGAAAGAATATTCATGCATGTACAA
TCTGTGGCAAGATGTTTCCATCACAGTCAAAACTTGATAGGCATGTACTTATTCATACTGGTCAGAGGCC
TTTTAAATGTGTCTTGTGTACTAAATCTTTTCGACAGTCAACTCACTTAAAAATCCACCAACTTACACAT
TCAGAAGAAAGACCTTTTCAATGTTGTTTTTGTCAAAAAGGATTTAAGATTCAAAGCAAACTTCTGAAGC
ATAAACAAATCCATACTAGGAATAAGGCTTTTCGGGCTCTTTTATTAAAGAAGAGGCGTACAGAATCTCG
CCCCCTGCCTAATAAGTTAAATGCAAATCAGGGTGGTTTTGAAAATGGTGAGATTGGTGAATCTGAGGAG
AATAATCCACTTGATGTCCACTCAATTTATATTGTCCCTTTTCAATGTCCAAAGTGTGAAAAGTGTTTTG
AATCAGAGCAGATTCTCAATGAACACAGCTGTTTTGCTGCTAGAAGTGGCAAAATTCCAAGCAGGTTCAA
AAGAAGCTACAACTATAAAACCATTGTTAAAAAAATCTTGGCCAAGCTTAAGCGTGCTAGGAGTAAAAAA
TTAGATAACTTTCAATCTGAGAAAAAAGTTTTTAAAAAGAGTTTCTTGAGAAATTGTGATCTTATTTCTG
GTGAGCAGAGCTCTGAACAAACCCAGAGAACATTTGTGGGTTCTCTTGGCAAACATGGAACATATAAAAC
AATTGGCAATAGAAAGAAGAAAACATTGACTTTGCCATTTTCTTGGCAAAATATGGGAAAAAATTTGAAA
GGCATCCTTACGACAGAAAACATATTAAGCATTGATAATTCAGTGAATAAGAAAGACTTGTCAATCTGTG
GTTCATCAGGTGAGGAATTCTTTAATAACTGTGAGGTACTTCAGTGTGGTTTTTCAGTTCCAAGGGAAAA
CATACGTACTAGACATAAGATATGTCCTTGTGACAAATGTGAGAAGGTATTTCCTTCTATATCCAAACTA
AAAAGACACTATTTAATTCATACTGGACAGAGGCCCTTTGGCTGTAATATTTGTGGGAAATCTTTTAGAC
AGTCAGCTCACTTAAAAAGACATGAACAGACTCATAATGAAAAGAGTCCTTATGCATCTCTTTGCCAAGT
AGAATTTGGAAACTTCAACAATCTTTCTAATCATTCAGGTAATAATGTTAACTATAATGCTTCCCAACAA
TGTCAGGCTCCTGGTGTTCAAAAATACGAGGTCTCAGAGTCAGATCAAATGTCAGGAGTTAAGGCAGAGT
CACAGGATTTTATTCCTGGTAGCACCGGGCAACCCTGTCTTCCTAATGTACTTTTGGAATCAGAGCAAAG
CAATCCTTTTTGCAGTTATTCAGAGCATCAGGAGAAAAATGATGTCTTCCTGTACCGATGCAGTGTTTGT
GCTAAAAGTTTCCGATCTCCATCTAAACTGGAAAGACACTACCTAATTCATGCAGGGCAGAAACCATTTG
AATGCTCAGTTTGTGGCAAAACATTCAGACAGGCTCCTCACTGGAAGAGACATCAGCTTACTCACTTTAA
AGAACGACCACAAGGGAAAGTGGTTGCCTTAGATTCGGTTATG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC209873 protein sequence
Red=Cloning site Green=Tags(s)

MMAENNLKMLKIQQCVVANKLPRNRPYVCNICFKHFETPSKLARHYLIHTGQKPFECDVCHKTFRQLVHL
ERHQLTHSLPFKCSICQRHFKNLKTFVKHQQLHNETYQNNVKQVRRLLEAKQEKSMYGVYNTFTTEERWA
LHPCSKSDPMYSMKRRKNIHACTICGKMFPSQSKLDRHVLIHTGQRPFKCVLCTKSFRQSTHLKIHQLTH
SEERPFQCCFCQKGFKIQSKLLKHKQIHTRNKAFRALLLKKRRTESRPLPNKLNANQGGFENGEIGESEE
NNPLDVHSIYIVPFQCPKCEKCFESEQILNEHSCFAARSGKIPSRFKRSYNYKTIVKKILAKLKRARSKK
LDNFQSEKKVFKKSFLRNCDLISGEQSSEQTQRTFVGSLGKHGTYKTIGNRKKKTLTLPFSWQNMGKNLK
GILTTENILSIDNSVNKKDLSICGSSGEEFFNNCEVLQCGFSVPRENIRTRHKICPCDKCEKVFPSISKL
KRHYLIHTGQRPFGCNICGKSFRQSAHLKRHEQTHNEKSPYASLCQVEFGNFNNLSNHSGNNVNYNASQQ
CQAPGVQKYEVSESDQMSGVKAESQDFIPGSTGQPCLPNVLLESEQSNPFCSYSEHQEKNDVFLYRCSVC
AKSFRSPSKLERHYLIHAGQKPFECSVCGKTFRQAPHWKRHQLTHFKERPQGKVVALDSVM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_014106
ORF Size 2073 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_014106.4
RefSeq Size 5453 bp
RefSeq ORF 2076 bp
Locus ID 54989
UniProt ID Q6IQ21
MW 80 kDa
Gene Summary May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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