United States
Germany
Japan
United Kingdom
China
DNA Polymerase lambda (POLL) (NM_013274) Human Tagged ORF Clone
CAT＃: RC209816
POLL (Myc-DDK-tagged)-Human polymerase (DNA directed), lambda (POLL), transcript variant 2
ORF Plasmid: tGFP
"NM_013274" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 4,280.00
CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BETAN; POLKAPPA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209816 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCCCAGGGGTATCTTGAAGGCATTTCCCAAGCGGCAGAAAATTCATGCTGATGCATCATCAAAAG TACTTGCAAAGATTCCTAGGAGGGAAGAGGGAGAAGAAGCAGAAGAGTGGCTGAGCTCCCTTCGGGCCCA TGTTGTGCGCACTGGCATTGGACGAGCCCGGGCAGAACTCTTTGAGAAGCAGATTGTTCAGCATGGCGGC CAGCTATGCCCTGCCCAGGGCCCAGGTGTCACTCACATTGTGGTGGATGAAGGCATGGACTATGAGCGAG CCCTCCGCCTTCTCAGACTACCCCAGCTGCCCCCGGGTGCTCAGCTGGTGAAGTCAGCCTGGCTGAGCTT GTGCCTTCAGGAGAGGAGGCTGGTGGATGTAGCTGGATTCAGCATCTTCATCCCCAGTAGGTACTTGGAC CATCCACAGCCCAGCAAGGCAGAGCAGGATGCTTCTATTCCTCCTGGCACCCATGAGGCCCTGCTTCAGA CAGCCCTTTCTCCTCCTCCTCCTCCCACCAGGCCTGTGTCTCCTCCCCAAAAGGCAAAAGAGGCACCAAA CACCCAAGCCCAGCCCATCTCTGATGATGAAGCCAGTGATGGGGAAGAAACCCAGGTTAGTGCAGCTGAT CTGGAAGCCCTCATCAGTGGCCACTACCCCACCTCCCTTGAGGGAGATTGTGAGCCTAGCCCAGCCCCTG CTGTCCTGGATAAGTGGGTCTGTGCACAGCCCTCAAGCCAGAAGGCGACCAATCACAACCTCCATATCAC AGAGAAGCTGGAAGTTCTGGCCAAAGCCTACAGTGTTCAGGGAGACAAGTGGAGGGCCCTGGGCTATGCC AAGGCCATCAATGCCCTCAAGAGCTTCCATAAGCCTGTCACCTCGTACCAGGGGGCCTGCAGTATCCCTG GGATTGGGAAGCGGATGGCTGAGAAAATCATAGAGATCCTGGAGAGCGGGCATTTGCGGAAGCTGGACCA TATCAGTGAGAGCGTGCCTGTCTTGGAGCTCTTCTCCAACATCTGGGGAGCTGGGACCAAGACTGCCCAG ATGTGGTACCAACAGGGCTTCCGAAGTCTGGAAGACATCCGCAGCCAGGCCTCCCTGACAACCCAGCAGG CCATCGGCCTGAAGCATTACAGTGACTTCCTGGAACGTATGCCCAGGGAGGAGGCTACAGAGATTGAGCA GACAGTCCAGAAAGCAGCCCAGGCCTTTAACTCTGGGCTGCTGTGTGTGGCATGTGGTTCATACCGACGG GGAAAGGCGACCTGTGGTGATGTCGACGTGCTCATCACTCACCCAGATGGCCGGTCCCACCGGGGTATCT TCAGCCGCCTCCTTGACAGTCTTCGGCAGGAAGGGTTCCTCACAGATGACTTGGTGAGCCAAGAGGAGAA TGGTCAGCAACAGAAGTACTTGGGGGTGTGCCGGCTCCCAGGGCCAGGGCGGCGGCACCGGCGCCTGGAC ATCATCGTGGTGCCCTATAGCGAGTTTGCCTGTGCCCTGCTCTACTTCACCGGCTCTGCACACTTCAACC GCTCCATGCGAGCCCTGGCCAAAACCAAGGGCATGAGTCTGTCAGAACATGCCCTCAGCACTGCTGTGGT CCGGAACACCCATGGCTGCAAGGTGGGGCCTGGCCGAGTGCTGCCCACTCCCACTGAGAAGGATGTCTTC AGGCTCTTAGGCCTCCCCTACCGAGAACCTGCTGAGCGGGACTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209816 protein sequence
Red=Cloning site Green=Tags(s) MDPRGILKAFPKRQKIHADASSKVLAKIPRREEGEEAEEWLSSLRAHVVRTGIGRARAELFEKQIVQHGG QLCPAQGPGVTHIVVDEGMDYERALRLLRLPQLPPGAQLVKSAWLSLCLQERRLVDVAGFSIFIPSRYLD HPQPSKAEQDASIPPGTHEALLQTALSPPPPPTRPVSPPQKAKEAPNTQAQPISDDEASDGEETQVSAAD LEALISGHYPTSLEGDCEPSPAPAVLDKWVCAQPSSQKATNHNLHITEKLEVLAKAYSVQGDKWRALGYA KAINALKSFHKPVTSYQGACSIPGIGKRMAEKIIEILESGHLRKLDHISESVPVLELFSNIWGAGTKTAQ MWYQQGFRSLEDIRSQASLTTQQAIGLKHYSDFLERMPREEATEIEQTVQKAAQAFNSGLLCVACGSYRR GKATCGDVDVLITHPDGRSHRGIFSRLLDSLRQEGFLTDDLVSQEENGQQQKYLGVCRLPGPGRRHRRLD IIVVPYSEFACALLYFTGSAHFNRSMRALAKTKGMSLSEHALSTAVVRNTHGCKVGPGRVLPTPTEKDVF RLLGLPYREPAERDW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013274 |
| ORF Size | 1725 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_013274.3 |
| RefSeq Size | 2741 bp |
| RefSeq ORF | 1728 bp |
| Locus ID | 27343 |
| UniProt ID | Q9UGP5 |
| Domains | POLXc |
| Protein Families | Druggable Genome |
| Protein Pathways | Base excision repair, Non-homologous end-joining |
| MW | 63.4 kDa |
| Gene Summary | This gene encodes a DNA polymerase. DNA polymerases catalyze DNA-template-directed extension of the 3'-end of a DNA strand. This particular polymerase, which is a member of the X family of DNA polymerases, likely plays a role in non-homologous end joining and other DNA repair processes. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209816L3 | Lenti ORF clone of Human polymerase (DNA directed), lambda (POLL), transcript variant 2, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC209816L4 | Lenti ORF clone of Human polymerase (DNA directed), lambda (POLL), transcript variant 2, mGFP tagged |
CNY 6,460.00 |
|
| RG209816 | POLL (tGFP-tagged) - Human polymerase (DNA directed), lambda (POLL), transcript variant 2 |
CNY 5,040.00 |
|
| SC115324 | POLL (untagged)-Human polymerase (DNA directed), lambda (POLL), transcript variant 2 |
CNY 4,288.00 |
其它DNA Polymerase lambda产品
