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HA1 (ARHGAP45) (NM_012292) Human Tagged ORF Clone
CAT＃: RC209595
HMHA1 (Myc-DDK-tagged)-Human histocompatibility (minor) HA-1 (HMHA1)
ORF Plasmid: tGFP
"NM_012292" in other vectors (6)
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CNY 7,912.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HA-1; HLA-HA1; HMHA1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209595 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCTCCAGGAAGAAACGAGAGCTCATGAAAACCCCTTCCATCTCGAAAAAGAACCGCGCGGGAAGCC CCAGCCCGCAGCCCTCGGGGGAGCTGCCCAGGAAGGATGGGGCTGACGCGGTGTTCCCCGGACCAAGCCT GGAGCCGCCCGCTGGGTCCTCCGGCGTCAAGGCCACAGGGACCCTCAAGCGGCCCACCAGCCTGAGCCGC CACGCCAGCGCGGCTGGCTTCCCCCTGTCGGGTGCTGCCTCCTGGACACTGGGCCGGAGCCACCGGAGCC CACTGACAGCCGCCAGCCCGGGCGAGCTGCCCACCGAGGGTGCCGGCCCGGACGTCGTCGAGGACATCTC CCATCTGCTGGCGGACGTGGCCCGCTTCGCTGAGGGCCTTGAGAAACTTAAGGAGTGTGTGCTGCATGAC GACCTCCTTGAGGCCCGCCGCCCGCGGGCCCACGAGTGCCTGGGTGAGGCTCTGCGTGTCATGCATCAGA TCATCTCCAAGTACCCGCTGCTGAACACCGTGGAGACGCTCACCGCAGCCGGCACCCTCATTGCCAAGGT CAAAGCCTTCCATTATGAGAGCAACAATGATCTGGAGAAACAGGAGTTCGAGAAGGCCCTGGAGACGATT GCTGTGGCCTTCAGTAGCACAGTGTCCGAGTTCCTCATGGGTGAAGTGGACAGCAGCACCCTCCTAGCAG TGCCTCCTGGGGACTCGAGCCAGTCCATGGAAAGCCTGTATGGACCGGGCAGTGAGGGCACGCCTCCCAG CCTGGATGACTGTGACGCCGGCTGCCTGCCCGCCGAGGAGGTGGACGTGCTGCTACAGCGCTGTGAGGGG GGCGTGGATGCCGCACTGCTGTATGCCAAGAACATGGCCAAGTACATGAAGGACCTCATCAGCTACCTGG AGAAGCGGACGACGCTGGAGATGGAGTTTGCCAAGGGCCTGCAGAAGATCGCTCACAACTGCAGACAGAG CGTCATGCAGGAGCCCCACATGCCGCTCCTGTCCATCTACTCGCTGGCCCTGGAGCAGGACCTGGAGTTC GGCCACAGCATGGTGCAGGCGGTGGGCACCTTGCAGACCCAGACCTTCATGCAGCCCCTGACCCTGCGGC GGCTTGAACACGAGAAGCGCAGGAAGGAGATCAAGGAGGCCTGGCACCGTGCCCAGAGGAAGCTGCAAGA GGCGGAGTCCAACCTGCGCAAGGCCAAGCAGGGTTACGTGCAGCGCTGCGAGGACCACGACAAGGCTCGC TTCCTCGTGGCCAAGGCGGAGGAGGAGCAGGCTGGCAGCGCGCCGGGAGCAGGCGGCACGGCCACCAAGA CCCTGGACAAGCGGCGGCGGCTGGAGGAGGAGGCCAAGAACAAGGCGGAGGAAGCTATGGCCACCTACCG CACCTGCGTGGCCGACGCGAAGACGCAGAAGCAGGAGCTGGAGGATACCAAGGTGACGGCGCTGCGGCAG ATCCAGGAGGTCATCCGGCAGAGCGACCAAACCATCAAGTCGGCCACGATCTCCTACTACCAGATGATGC ATATGCAGACGGCGCCGCTGCCCGTGCACTTCCAGATGCTGTGTGAGAGCAGCAAGCTGTATGACCCAGG CCAGCAGTACGCCTCCCACGTGCGCCAGCTGCAGCGGGACCAGGAGCCCGATGTGCACTACGACTTTGAG CCCCACGTCTCCGCCAACGCCTGGTCCCCCGTCATGCGTGCCCGGAAGAGCAGCTTCAACGTGAGTGATG TGGCGCGGCCCGAGGCTGCCGGGAGCCCCCCAGAAGAAGGCGGGTGCACTGAGGGCACACCTGCCAAGGA CCACAGGGCCGGGCGAGGACACCAGGTTCACAAGTCATGGCCGCTCTCGATCTCAGACTCGGACAGTGGG CTGGACCCCGGCCCTGGCGCAGGGGACTTTAAGAAGTTCGAGCGGACGTCATCCAGTGGTACCATGTCGT CCACGGAGGAGCTGGTGGACCCAGACGGTGGAGCCGGGGCTTCAGCCTTTGAGCAGGCTGACCTCAACGG CATGACCCCCGAGCTGCCGGTGGCCGTGCCCAGTGGACCGTTCCGCCACGAGGGGCTGTCCAAGGCGGCC CGTACTCACCGGCTCCGGAAGCTCCGCACGCCCGCCAAGTGCCGCGAGTGCAACAGCTACGTCTACTTCC AGGGTGCTGAGTGTGAAGAGTGCTGCCTGGCCTGCCACAAGAAATGTCTGGAGACGCTGGCCATACAGTG CGGGCACAAGAAGCTGCAGGGCCGCCTGCAGCTGTTCGGCCAGGACTTCAGCCACGCGGCCCGCAGCGCC CCCGACGGCGTGCCCTTCATCGTCAAGAAGTGCGTCTGCGAGATCGAGCGGCGGGCGCTGCGCACCAAGG GCATCTACCGGGTCAATGGGGTAAAGACACGCGTGGAGAAGCTGTGCCAGGCCTTCGAGAACGGCAAGGA GCTGGTCGAGCTGTCGCAGGCCTCGCCCCACGACATCAGCAACGTCCTCAAGCTCTACCTGCGTCAGCTT CCCGAGCCGCTCATCTCCTTCCGCCTCTACCACGAGCTCGTAGGGCTGGCCAAGGACAGCCTGAAGGCAG AGGCCGAGGCCAAGGCGGCGTCCCGGGGCCGGCAGGACGGCTCGGAGAGCGAGGCAGTGGCGGTGGCCCT GGCAGGTCGGCTGCGGGAGCTCCTGCGGGACCTGCCGCCTGAGAACCGGGCCTCGCTGCAGTACCTGCTG CGTCACCTACGCAGGATCGTGGAGGTGGAGCAGGACAACAAGATGACCCCCGGGAACCTGGGCATCGTGT TCGGGCCCACGCTGCTTCGGCCACGGCCCACCGAGGCCACCGTGTCCCTCTCCTCCCTGGTGGATTATCC CCATCAGGCCCGCGTCATCGAGACTCTCATCGTCCACTACGGCCTGGTCTTCGAGGAGGAGCCGGAGGAG ACCCCCGGGGGCCAGGACGAGTCATCCAACCAGCGAGCTGAGGTAGTCGTCCAGGTGCCGTACCTGGAGG CGGGCGAGGCGGTGGTCTACCCGCTGCAGGAGGCGGCGGCGGACGGGTGCAGAGAATCCCGAGTTGTGTC CAACGATTCGGACTCGGACCTAGAGGAGGCCTCCGAGCTGCTGTCCTCATCGGAGGCCAGTGCCCTGGGC CACCTCAGCTTCCTGGAGCAGCAGCAGAGCGAGGCCAGCCTAGAGGTGGCTTCTGGCAGCCACAGCGGCA GTGAGGAGCAGCTGGAGGCCACAGCCCGGGAGGACGGGGACGGGGACGAGGACGGCCCGGCCCAGCAGCT CTCAGGATTCAACACCAACCAGTCCAACAACGTGCTGCAGGCCCCACTGCCCCCCATGAGGCTCCGTGGC GGGCGGATGACACTGGGCTCCTGCAGGGAAAGGCAGCCGGAATTCGTG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC209595 protein sequence
Red=Cloning site Green=Tags(s) MFSRKKRELMKTPSISKKNRAGSPSPQPSGELPRKDGADAVFPGPSLEPPAGSSGVKATGTLKRPTSLSR HASAAGFPLSGAASWTLGRSHRSPLTAASPGELPTEGAGPDVVEDISHLLADVARFAEGLEKLKECVLHD DLLEARRPRAHECLGEALRVMHQIISKYPLLNTVETLTAAGTLIAKVKAFHYESNNDLEKQEFEKALETI AVAFSSTVSEFLMGEVDSSTLLAVPPGDSSQSMESLYGPGSEGTPPSLDDCDAGCLPAEEVDVLLQRCEG GVDAALLYAKNMAKYMKDLISYLEKRTTLEMEFAKGLQKIAHNCRQSVMQEPHMPLLSIYSLALEQDLEF GHSMVQAVGTLQTQTFMQPLTLRRLEHEKRRKEIKEAWHRAQRKLQEAESNLRKAKQGYVQRCEDHDKAR FLVAKAEEEQAGSAPGAGGTATKTLDKRRRLEEEAKNKAEEAMATYRTCVADAKTQKQELEDTKVTALRQ IQEVIRQSDQTIKSATISYYQMMHMQTAPLPVHFQMLCESSKLYDPGQQYASHVRQLQRDQEPDVHYDFE PHVSANAWSPVMRARKSSFNVSDVARPEAAGSPPEEGGCTEGTPAKDHRAGRGHQVHKSWPLSISDSDSG LDPGPGAGDFKKFERTSSSGTMSSTEELVDPDGGAGASAFEQADLNGMTPELPVAVPSGPFRHEGLSKAA RTHRLRKLRTPAKCRECNSYVYFQGAECEECCLACHKKCLETLAIQCGHKKLQGRLQLFGQDFSHAARSA PDGVPFIVKKCVCEIERRALRTKGIYRVNGVKTRVEKLCQAFENGKELVELSQASPHDISNVLKLYLRQL PEPLISFRLYHELVGLAKDSLKAEAEAKAASRGRQDGSESEAVAVALAGRLRELLRDLPPENRASLQYLL RHLRRIVEVEQDNKMTPGNLGIVFGPTLLRPRPTEATVSLSSLVDYPHQARVIETLIVHYGLVFEEEPEE TPGGQDESSNQRAEVVVQVPYLEAGEAVVYPLQEAAADGCRESRVVSNDSDSDLEEASELLSSSEASALG HLSFLEQQQSEASLEVASGSHSGSEEQLEATAREDGDGDEDGPAQQLSGFNTNQSNNVLQAPLPPMRLRG GRMTLGSCRERQPEFV SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012292 |
| ORF Size | 3408 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012292.1 |
| RefSeq Size | 5488 bp |
| RefSeq ORF | 3411 bp |
| Locus ID | 23526 |
| UniProt ID | Q92619 |
| MW | 124.6 kDa |
| Gene Summary | Contains a GTPase activator for the Rho-type GTPases (RhoGAP) domain that would be able to negatively regulate the actin cytoskeleton as well as cell spreading. However, also contains N-terminally a BAR-domin which is able to play an autoinhibitory effect on this RhoGAP activity.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209595L1 | Lenti ORF clone of Human histocompatibility (minor) HA-1 (HMHA1), Myc-DDK-tagged |
CNY 10,312.00 |
|
| RC209595L2 | Lenti ORF clone of Human histocompatibility (minor) HA-1 (HMHA1), mGFP tagged |
CNY 13,210.00 |
|
| RC209595L3 | Lenti ORF clone of Human histocompatibility (minor) HA-1 (HMHA1), Myc-DDK-tagged |
CNY 13,210.00 |
|
| RC209595L4 | Lenti ORF clone of Human histocompatibility (minor) HA-1 (HMHA1), mGFP tagged |
CNY 13,210.00 |
|
| RG209595 | HMHA1 (tGFP-tagged) - Human histocompatibility (minor) HA-1 (HMHA1) |
CNY 9,512.00 |
|
| SC303952 | HMHA1 (untagged)-Human histocompatibility (minor) HA-1 (HMHA1) |
CNY 10,450.00 |
