COQ9 (NM_020312) Human Tagged ORF Clone

CAT#: RC209578

COQ9 (Myc-DDK-tagged)-Human coenzyme Q9 homolog (S. cerevisiae) (COQ9), nuclear gene encoding mitochondrial protein

ORF Plasmid: DDK tGFP



  "NM_020312" in other vectors (4)

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CNY 2,400.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


COQ9 Antibody - N-terminal region
    • 100 ul

CNY 5,250.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms C16orf49; COQ10D5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC209578 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGGCGGCGGTATCTGGTGCGCTTGGCCGGGCGGGCTGGAGGCTCCTGCAGCTGCGATGCCTGC
CCGTGGCCCGTTGCCGACAAGCCCTGGTGCCGCGTGCCTTCCATGCTTCAGCTGTGGGGCTAAGGTCTTC
AGATGAGCAGAAGCAGCAGCCTCCCAACTCATTTTCTCAGCAGCATTCTGAGACACAGGGGGCAGAAAAA
CCTGATCCAGAGTCTTCTCATTCACCCCCCAGGTATACAGACCAGGGCGGCGAGGAGGAGGAGGACTATG
AAAGTGAGGAGCAGTTGCAGCACCGCATCCTGACGGCAGCCCTTGAGTTTGTGCCCGCCCACGGGTGGAC
AGCAGAGGCGATTGCAGAAGGAGCCCAGTCTCTGGGTCTCTCCAGTGCAGCAGCCAGCATGTTCGGGAAG
GATGGCAGTGAGCTAATACTGCATTTTGTGACCCAGTGCAATACCCGGCTCACACGTGTGCTAGAAGAGG
AGCAGAAGCTGGTACAGTTGGGCCAGGCGGAGAAGAGGAAGACAGACCAGTTCCTGAGGGATGCAGTGGA
AACCAGACTGAGAATGCTGATCCCATACATTGAGCACTGGCCCCGGGCCCTCAGCATCCTCATGCTCCCT
CACAACATCCCGTCCAGCCTGAGCCTGCTCACCAGCATGGTGGATGACATGTGGCATTACGCTGGGGACC
AGTCCACTGATTTTAACTGGTACACCCGCCGAGCCATGCTGGCTGCCATCTACAACACAACAGAGCTGGT
GATGATGCAGGACTCCTCTCCAGACTTTGAGGACACTTGGCGCTTCCTGGAAAACCGGGTTAATGATGCA
ATGAACATGGGCCACACTGCCAAGCAGGTAAAGTCCACAGGAGAGGCACTGGTGCAAGGACTCATGGGTG
CAGCAGTGACGCTCAAGAACTTGACAGGTCTAAACCAGCGTCGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC209578 protein sequence
Red=Cloning site Green=Tags(s)

MAAAAVSGALGRAGWRLLQLRCLPVARCRQALVPRAFHASAVGLRSSDEQKQQPPNSFSQQHSETQGAEK
PDPESSHSPPRYTDQGGEEEEDYESEEQLQHRILTAALEFVPAHGWTAEAIAEGAQSLGLSSAAASMFGK
DGSELILHFVTQCNTRLTRVLEEEQKLVQLGQAEKRKTDQFLRDAVETRLRMLIPYIEHWPRALSILMLP
HNIPSSLSLLTSMVDDMWHYAGDQSTDFNWYTRRAMLAAIYNTTELVMMQDSSPDFEDTWRFLENRVNDA
MNMGHTAKQVKSTGEALVQGLMGAAVTLKNLTGLNQRR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_020312
ORF Size 954 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_020312.4
RefSeq Size 1699 bp
RefSeq ORF 957 bp
Locus ID 57017
UniProt ID O75208
Protein Families Druggable Genome
MW 35.5 kDa
Gene Summary This locus represents a mitochondrial ubiquinone biosynthesis gene. The encoded protein is likely necessary for biosynthesis of coenzyme Q10, as mutations at this locus have been associated with autosomal-recessive neonatal-onset primary coenzyme Q10 deficiency.[provided by RefSeq, Sep 2010]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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