NAB2 (NM_005967) Human Tagged ORF Clone

CAT#: RC209554

NAB2 (Myc-DDK-tagged)-Human NGFI-A binding protein 2 (EGR1 binding protein 2) (NAB2)

ORF Plasmid: DDK tGFP



  "NM_005967" in other vectors (7)

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CNY 5,864.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


NAB2 mouse monoclonal antibody, clone OTI5D7 (formerly 5D7)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms MADER
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC209554 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCACAGAGCGCCTTCCCCCACAGCCGAGCAGCCGCCGGGCGGAGGGGACAGCGCCCGCCGGACCCTGC
AGCCCAGACTCAAGCCCAGTGCCCGAGCCATGGCACTGCCTCGGACGCTGGGGGAGCTGCAGCTGTACCG
GGTCCTGCAGCGCGCCAACCTCCTTTCCTACTATGAGACCTTCATCCAGCAGGGAGGGGACGACGTGCAG
CAGCTGTGTGAGGCGGGTGAGGAGGAGTTTCTGGAGATCATGGCACTTGTGGGCATGGCCACCAAGCCCC
TCCATGTCCGGCGCCTGCAGAAGGCACTGAGAGAGTGGGCCACCAATCCAGGGCTCTTCAGTCAACCAGT
GCCTGCTGTTCCCGTCTCCAGCATCCCGCTCTTCAAGATCTCTGAGACTGCGGGTACCCGGAAAGGGAGC
ATGAGCAATGGGCATGGCAGCCCAGGGGAAAAGGCAGGCAGTGCCCGCAGTTTTAGCCCCAAGAGCCCCC
TTGAACTTGGAGAGAAGCTATCACCACTGCCTGGGGGACCTGGGGCAGGGGACCCCCGGATCTGGCCAGG
CCGGAGCACTCCAGAGTCGGACGTTGGGGCAGGAGGAGAAGAGGAGGCTGGCTCGCCCCCCTTCTCCCCC
CCTGCAGGGGGAGGAGTCCCTGAGGGGACTGGGGCTGGGGGGCTGGCAGCAGGTGGGACTGGGGGTGGTC
CAGACCGACTGGAGCCAGAGATGGTACGCATGGTGGTGGAAAGTGTGGAGAGGATCTTCCGGAGCTTCCC
AAGGGGGGATGCTGGGGAGGTCACATCCCTGCTAAAGCTGAATAAGAAGCTGGCACGGAGCGTTGGGCAC
ATCTTTGAGATGGATGATAATGACAGCCAGAAGGAAGAGGAGATCCGCAAATACAGCATCATCTATGGCC
GTTTCGACTCTAAGCGGCGGGAGGGCAAGCAGCTCAGCCTGCACGAGCTCACCATCAACGAGGCTGCTGC
CCAGTTCTGCATGAGGGACAACACGCTCTTATTACGGAGAGTGGAGCTCTTCTCTTTGTCCCGCCAAGTA
GCCCGAGAGAGCACCTACTTGTCCTCCTTGAAGGGCTCCAGGCTTCACCCTGAAGAACTGGGAGGCCCTC
CACTGAAGAAGCTGAAACAAGAGGTTGGAGAACAGAGTCACCCTGAAATCCAGCAGCCTCCCCCAGGCCC
TGAGTCCTATGTACCCCCATACCGCCCCAGCCTGGAGGAGGACAGCGCCAGCCTGTCTGGGGAGAGTCTG
GATGGACATTTGCAGGCTGTGGGGTCATGTCCAAGGCTGACGCCGCCCCCTGCTGACCTGCCTCTGGCAT
TGCCAGCCCATGGGCTATGGAGCCGACACATCCTGCAGCAGACACTGATGGACGAGGGGCTGCGGCTCGC
CCGCCTCGTCTCCCACGACCGCGTGGGCCGCCTCAGCCCCTGTGTGCCTGCGAAGCCACCTCTCGCAGAG
TTCGAGGAAGGGCTGCTGGACAGATGTCCTGCCCCAGGACCCCATCCCGCGCTGGTGGAGGGTCGCAGGA
GCAGCGTGAAAGTGGAGGCTGAGGCCAGCCGGCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC209554 protein sequence
Red=Cloning site Green=Tags(s)

MHRAPSPTAEQPPGGGDSARRTLQPRLKPSARAMALPRTLGELQLYRVLQRANLLSYYETFIQQGGDDVQ
QLCEAGEEEFLEIMALVGMATKPLHVRRLQKALREWATNPGLFSQPVPAVPVSSIPLFKISETAGTRKGS
MSNGHGSPGEKAGSARSFSPKSPLELGEKLSPLPGGPGAGDPRIWPGRSTPESDVGAGGEEEAGSPPFSP
PAGGGVPEGTGAGGLAAGGTGGGPDRLEPEMVRMVVESVERIFRSFPRGDAGEVTSLLKLNKKLARSVGH
IFEMDDNDSQKEEEIRKYSIIYGRFDSKRREGKQLSLHELTINEAAAQFCMRDNTLLLRRVELFSLSRQV
ARESTYLSSLKGSRLHPEELGGPPLKKLKQEVGEQSHPEIQQPPPGPESYVPPYRPSLEEDSASLSGESL
DGHLQAVGSCPRLTPPPADLPLALPAHGLWSRHILQQTLMDEGLRLARLVSHDRVGRLSPCVPAKPPLAE
FEEGLLDRCPAPGPHPALVEGRRSSVKVEAEASRQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_005967
ORF Size 1575 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005967.4
RefSeq Size 2725 bp
RefSeq ORF 1578 bp
Locus ID 4665
UniProt ID Q15742
Domains NCD1, NCD2
Protein Families Transcription Factors
MW 56.6 kDa
Gene Summary This gene encodes a member of the family of NGFI-A binding (NAB) proteins, which function in the nucleus to repress transcription induced by some members of the EGR (early growth response) family of transactivators. NAB proteins can homo- or hetero-multimerize with other EGR or NAB proteins through a conserved N-terminal domain, and repress transcription through two partially redundant C-terminal domains. Transcriptional repression by the encoded protein is mediated in part by interactions with the nucleosome remodeling and deactylase (NuRD) complex. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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