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PRMT6 (NM_018137) Human Tagged ORF Clone
CAT＃: RC209527
PRMT6 (Myc-DDK-tagged)-Human protein arginine methyltransferase 6 (PRMT6)
ORF Plasmid: tGFP
"NM_018137" in other vectors (11)
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CNY 2,400.00
CNY 4,370.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HRMT1L6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209527 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCGCGGACCGCGTCCGCACCGATGCCTACCGCCTGGGTATCCTTCGGAACTGGGCAGCACTGCGAG GCAAGACGGTACTGGACGTGGGCGCGGGCACCGGCATTCTGAGCATCTTCTGTGCCCAGGCCGGGGCCCG GCGCGTGTACGCGGTAGAGGCCAGCGCCATCTGGCAACAGGCCCGGGAGGTGGTGCGGTTCAACGGGCTG GAGGACCGGGTGCACGTCCTGCCGGGACCAGTGGAGACTGTAGAGTTGCCGGAACAGGTGGATGCCATCG TGAGCGAGTGGATGGGCTACGGACTCCTGCACGAGTCCATGCTGAGCTCCGTCCTCCACGCGCGAACCAA GTGGCTGAAGGAGGGCGGTCTTCTCCTGCCGGCCTCCGCCGAGCTCTTCATAGCCCCCATCAGCGACCAG ATGCTGGAATGGCGCCTGGGCTTCTGGAGCCAGGTGAAGCAGCACTATGGTGTGGACATGAGCTGCCTGG AGGGCTTCGCCACGCGCTGTCTCATGGGCCACTCGGAGATCGTTGTGCAGGGATTGTCCGGCGAGGACGT GCTGGCCCGGCCGCAGCGCTTTGCTCAGCTAGAGCTCTCCCGCGCCGGCTTGGAGCAGGAGCTGGAGGCC GGAGTGGGCGGGCGCTTCCGCTGCAGCTGCTATGGCTCGGCGCCCATGCATGGCTTTGCCATCTGGTTCC AGGTGACCTTCCCTGGAGGGGAGTCGGAGAAACCCCTGGTGCTGTCCACCTCGCCTTTTCACCCGGCCAC TCACTGGAAACAGGCGCTCCTCTACCTGAACGAGCCGGTGCAAGTGGAGCAAGACACGGACGTTTCAGGA GAGATCACGCTGCTGCCCTCCCGGGACAACCCCCGTCGCCTGCGCGTGCTGCTGCGCTACAAAGTGGGAG ACCAGGAGGAGAAGACCAAAGACTTTGCCATGGAGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209527 protein sequence
Red=Cloning site Green=Tags(s) MIADRVRTDAYRLGILRNWAALRGKTVLDVGAGTGILSIFCAQAGARRVYAVEASAIWQQAREVVRFNGL EDRVHVLPGPVETVELPEQVDAIVSEWMGYGLLHESMLSSVLHARTKWLKEGGLLLPASAELFIAPISDQ MLEWRLGFWSQVKQHYGVDMSCLEGFATRCLMGHSEIVVQGLSGEDVLARPQRFAQLELSRAGLEQELEA GVGGRFRCSCYGSAPMHGFAIWFQVTFPGGESEKPLVLSTSPFHPATHWKQALLYLNEPVQVEQDTDVSG EITLLPSRDNPRRLRVLLRYKVGDQEEKTKDFAMED TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018137 |
| ORF Size | 948 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_018137.1, NP_060607.1 |
| RefSeq Size | 2665 bp |
| RefSeq ORF | 1128 bp |
| Locus ID | 55170 |
| UniProt ID | Q96LA8 |
| Protein Families | Druggable Genome |
| MW | 35.2 kDa |
| Gene Summary | The protein encoded by this gene belongs to the arginine N-methyltransferase family, which catalyze the sequential transfer of methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins, to form methylated arginine derivatives and S-adenosyl-L-homocysteine. This protein can catalyze both, the formation of omega-N monomethylarginine and asymmetrical dimethylarginine, with a strong preference for the latter. It specifically mediates the asymmetric dimethylation of Arg2 of histone H3, and the methylated form represents a specific tag for epigenetic transcriptional repression. This protein also forms a complex with, and methylates DNA polymerase beta, resulting in stimulation of polymerase activity by enhancing DNA binding and processivity. [provided by RefSeq, Sep 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209527L3 | Lenti ORF clone of Human protein arginine methyltransferase 6 (PRMT6), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC209527L4 | Lenti ORF clone of Human protein arginine methyltransferase 6 (PRMT6), mGFP tagged |
CNY 4,800.00 |
|
| RC229177 | PRMT6 (Myc-DDK-tagged)-Human protein arginine methyltransferase 6 (PRMT6) |
CNY 3,656.00 |
|
| RC229177L1 | Lenti ORF clone of Human protein arginine methyltransferase 6 (PRMT6), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC229177L2 | Lenti ORF clone of Human protein arginine methyltransferase 6 (PRMT6), mGFP tagged |
CNY 5,890.00 |
|
| RC229177L3 | Lenti ORF clone of Human protein arginine methyltransferase 6 (PRMT6), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC229177L4 | Lenti ORF clone of Human protein arginine methyltransferase 6 (PRMT6), mGFP tagged |
CNY 6,056.00 |
|
| RG209527 | PRMT6 (tGFP-tagged) - Human protein arginine methyltransferase 6 (PRMT6) |
CNY 4,000.00 |
|
| RG229177 | PRMT6 (tGFP-tagged) - Human protein arginine methyltransferase 6 (PRMT6) |
CNY 4,370.00 |
|
| SC111320 | PRMT6 (untagged)-Human protein arginine methyltransferase 6 (PRMT6) |
CNY 6,080.00 |
|
| SC327812 | PRMT6 (untagged)-Human protein arginine methyltransferase 6 (PRMT6) |
CNY 6,080.00 |
