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SP1 (NM_138473) Human Tagged ORF Clone
CAT＃: RC209154
SP1 (Myc-DDK-tagged)-Human Sp1 transcription factor (SP1), transcript variant 1
ORF Plasmid: tGFP
"NM_138473" in other vectors (6)
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CNY 8,624.00
| Cited in 9 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209154 representing NM_138473.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGCGACCAAGATCACTCCATGGATGAAATGACAGCTGTGGTGAAAATTGAAAAAGGAGTTGGTGGC AATAATGGGGGCAATGGTAATGGTGGTGGTGCCTTTTCACAGGCTCGAAGTAGCAGCACAGGCAGTAGC AGCAGCACTGGAGGAGGAGGGCAGGAGTCCCAGCCATCCCCTTTGGCTCTGCTGGCAGCAACTTGCAGC AGAATTGAGTCACCCAATGAGAACAGCAACAACTCCCAGGGCCCGAGTCAGTCAGGGGGAACAGGTGAG CTTGACCTCACAGCCACACAACTTTCACAGGGTGCCAATGGCTGGCAGATCATCTCTTCCTCCTCTGGG GCTACCCCTACCTCAAAGGAACAGAGTGGCAGCAGTACCAATGGCAGCAATGGCAGTGAGTCTTCCAAG AATCGCACAGTCTCTGGTGGGCAGTATGTTGTGGCTGCCGCTCCCAACTTACAGAACCAGCAAGTTCTG ACAGGACTACCTGGAGTGATGCCTAATATTCAGTATCAAGTAATCCCACAGTTCCAGACCGTTGATGGG CAACAGCTGCAGTTTGCTGCCACTGGGGCCCAAGTGCAGCAGGATGGTTCTGGTCAAATACAGATCATA CCAGGTGCAAACCAACAGATTATCACAAATCGAGGAAGTGGAGGCAACATCATTGCTGCTATGCCAAAC CTACTCCAGCAGGCTGTCCCCCTCCAAGGCCTGGCTAATAATGTACTCTCAGGACAGACTCAGTATGTG ACCAATGTACCAGTGGCCCTGAATGGGAACATCACCTTGCTACCTGTCAACAGCGTTTCTGCAGCTACC TTGACTCCCAGCTCTCAGGCAGTCACGATCAGCAGCTCTGGGTCCCAGGAGAGTGGCTCACAGCCTGTC ACCTCAGGGACTACCATCAGTTCTGCCAGCTTGGTATCATCACAAGCCAGTTCCAGCTCCTTTTTCACC AATGCCAATAGCTACTCAACTACTACTACCACCAGCAACATGGGAATTATGAACTTTACTACCAGTGGA TCATCAGGGACCAACTCTCAAGGCCAGACACCCCAGAGGGTCAGTGGGCTACAGGGGTCTGATGCTCTG AACATCCAGCAAAACCAGACATCTGGAGGCTCATTGCAAGCAGGCCAGCAAAAAGAAGGAGAGCAAAAC CAGCAGACACAGCAGCAACAAATTCTTATCCAGCCTCAGCTAGTTCAAGGGGGACAGGCCCTCCAGGCC CTCCAAGCAGCACCATTGTCAGGGCAGACCTTTACAACTCAAGCCATCTCCCAGGAAACCCTCCAGAAC CTCCAGCTTCAGGCTGTTCCAAACTCTGGTCCCATCATCATCCGGACACCAACAGTGGGGCCCAATGGA CAGGTCAGTTGGCAGACTCTACAGCTGCAGAACCTCCAAGTTCAGAACCCACAAGCCCAAACAATCACC TTAGCCCCAATGCAGGGTGTTTCCTTGGGGCAGACCAGCAGCAGCAACACCACTCTCACACCCATTGCC TCAGCTGCTTCCATTCCTGCTGGCACAGTCACTGTGAATGCTGCTCAACTCTCCTCCATGCCAGGCCTC CAGACCATTAACCTCAGTGCATTGGGTACTTCAGGAATCCAGGTGCACCCAATTCAAGGCCTGCCGTTG GCTATAGCAAATGCCCCAGGTGATCATGGAGCTCAGCTTGGTCTCCATGGGGCTGGTGGTGATGGAATA CATGATGACACAGCAGGTGGAGAGGAAGGAGAAAACAGCCCAGATGCCCAACCCCAAGCCGGTCGGAGG ACCCGGCGGGAAGCATGCACCTGCCCCTACTGTAAAGACAGTGAAGGAAGGGGCTCGGGGGATCCTGGC AAAAAGAAACAGCATATTTGCCACATCCAAGGCTGTGGGAAAGTGTATGGCAAGACCTCTCACCTGCGG GCACACTTGCGCTGGCATACAGGCGAGAGGCCATTTATGTGTACCTGGTCATACTGTGGGAAACGCTTC ACACGTTCGGATGAGCTACAGAGGCACAAACGTACACACACAGGTGAGAAGAAATTTGCCTGCCCTGAG TGTCCTAAGCGCTTCATGAGGAGTGACCACCTGTCAAAACATATCAAGACCCACCAGAATAAGAAGGGA GGCCCAGGTGTAGCTCTGAGTGTGGGCACTTTGCCCCTGGACAGTGGGGCAGGTTCAGAAGGCAGTGGC ACTGCCACTCCTTCAGCCCTTATTACCACCAATATGGTAGCCATGGAGGCCATCTGTCCAGAGGGCATT GCCCGTCTTGCCAACAGTGGCATCAACGTCATGCAGGTGGCAGATCTGCAGTCCATTAATATCAGTGGC AATGGCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC >Peptide sequence encoded by RC209154
Blue=ORF Red=Cloning site Green=Tag(s) MSDQDHSMDEMTAVVKIEKGVGGNNGGNGNGGGAFSQARSSSTGSSSSTGGGGQESQPSPLALLAATCS RIESPNENSNNSQGPSQSGGTGELDLTATQLSQGANGWQIISSSSGATPTSKEQSGSSTNGSNGSESSK NRTVSGGQYVVAAAPNLQNQQVLTGLPGVMPNIQYQVIPQFQTVDGQQLQFAATGAQVQQDGSGQIQII PGANQQIITNRGSGGNIIAAMPNLLQQAVPLQGLANNVLSGQTQYVTNVPVALNGNITLLPVNSVSAAT LTPSSQAVTISSSGSQESGSQPVTSGTTISSASLVSSQASSSSFFTNANSYSTTTTTSNMGIMNFTTSG SSGTNSQGQTPQRVSGLQGSDALNIQQNQTSGGSLQAGQQKEGEQNQQTQQQQILIQPQLVQGGQALQA LQAAPLSGQTFTTQAISQETLQNLQLQAVPNSGPIIIRTPTVGPNGQVSWQTLQLQNLQVQNPQAQTIT LAPMQGVSLGQTSSSNTTLTPIASAASIPAGTVTVNAAQLSSMPGLQTINLSALGTSGIQVHPIQGLPL AIANAPGDHGAQLGLHGAGGDGIHDDTAGGEEGENSPDAQPQAGRRTRREACTCPYCKDSEGRGSGDPG KKKQHICHIQGCGKVYGKTSHLRAHLRWHTGERPFMCTWSYCGKRFTRSDELQRHKRTHTGEKKFACPE CPKRFMRSDHLSKHIKTHQNKKGGPGVALSVGTLPLDSGAGSEGSGTATPSALITTNMVAMEAICPEGI ARLANSGINVMQVADLQSINISGNGF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_138473 |
| ORF Size | 2355 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_138473.3 |
| RefSeq Size | 7667 bp |
| RefSeq ORF | 2358 bp |
| Locus ID | 6667 |
| UniProt ID | P08047 |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Stem cell - Pluripotency, Stem cell relevant signaling - JAK/STAT signaling pathway, Transcription Factors |
| Protein Pathways | Huntington's disease, TGF-beta signaling pathway |
| MW | 80.7 kDa |
| Gene Summary | The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014] |
Citations (9)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Increased expression of BCL11B and its recruited chromatin remodeling factors during highly active antiretroviral therapy synergistically represses the transcription of human immunodeficiency virus type 1 and is associated with residual immune activation
,Wang, J;Yang, Z;Wu, NP;Yang, J;,
Arch. Virol.
,PubMed ID 31828511
[SP1]
|
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SP1 regulates KLF4 via SP1 binding motif governed by DNA methylation during odontoblastic differentiation of human dental pulp cells
,Sun, Z;Yu, S;Chen, S;Liu, H;Chen, Z;,
J. Cell. Biochem.
,PubMed ID 31009133
[SP1]
|
|
Mutant KRAS promotes liver metastasis of colorectal cancer, in part, by upregulating the MEK-Sp1-DNMT1-miR-137-YB-1-IGF-IR signaling pathway
,Chu, PC;Lin, PC;Wu, HY;Lin, KT;Wu, C;Bekaii-Saab, T;Lin, YJ;Lee, CT;Lee, JC;Chen, CS;,
Oncogene
,PubMed ID 29559746
[SP1]
|
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A type 2 diabetes-associated SNP in KCNQ1 (rs163184) modulates the binding activity of the locus for Sp3 and Lsd1/Kdm1a, potentially affecting CDKN1C expression
,null,
International Journal of Molecular Medicine
,PubMed ID 29207083
[SP1]
|
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Activation of ERK and Mutual Regulation of Stat3 and SP1 Contribute to Inhibition of PDK1 Expression by Atractylenolide-1 in Human Lung Cancer Cells
,Xiao, Q;Zheng, F;Wu, J;Tang, Q;Wang, W;Hann, SS;,
Cell. Physiol. Biochem.
,PubMed ID 29073620
[SP1]
|
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MicroRNA-141 enhances anoikis resistance in metastatic progression of ovarian cancer through targeting KLF12/Sp1/survivin axis
,Mak, CS;Yung, MM;Hui, LM;Leung, LL;Liang, R;Chen, K;Liu, SS;Qin, Y;Leung, TH;Lee, KF;Chan, KK;Ngan, HY;Chan, DW;,
Mol. Cancer
,PubMed ID 28095864
[Sp1]
|
|
A functional variant in the UBE2B gene promoter is associated with idiopathic azoospermia
,Mou, L;Zhang, Q;Diao, R;Cai, Z;Gui, Y;,
Reprod. Biol. Endocrinol.
,PubMed ID 26223869
[SP1]
|
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Differential effects of Sp cellular transcription factors on viral promoter activation by varicella-zoster virus (VZV) IE62 protein
,Khalil, MI;Ruyechan, WT;Hay, J;Arvin, A;,
Virology
,PubMed ID 26207799
[SP1]
|
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The Cyclin-Dependent Kinase Inhibitor p16INK4a Physically Interacts with the Transcription Factor Sp1 and the Cyclin-Dependent Kinase 4 to Transactivate microRNA-141 and microRNA-146b-5p Spontaneously and in Response to UV-induced DNA Damage
,Al-Khalaf, HH;Mohideen, P;Nallar, SC;Kalvakolanu, DV;Aboussekhra, A;,
J Biol Chem. 2013 Oct 27
,PubMed ID 24163379
[SP1]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209154L1 | Lenti ORF clone of Human Sp1 transcription factor (SP1), transcript variant 1, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC209154L2 | Lenti ORF clone of Human Sp1 transcription factor (SP1), transcript variant 1, mGFP tagged |
CNY 11,024.00 |
|
| RC209154L3 | Lenti ORF clone of Human Sp1 transcription factor (SP1), transcript variant 1, Myc-DDK-tagged |
CNY 11,024.00 |
|
| RC209154L4 | Lenti ORF clone of Human Sp1 transcription factor (SP1), transcript variant 1, mGFP tagged |
CNY 11,024.00 |
|
| RG209154 | SP1 (tGFP-tagged) - Human Sp1 transcription factor (SP1), transcript variant 1 |
CNY 10,224.00 |
|
| SC101137 | SP1 (untagged)-Human Sp1 transcription factor (SP1), transcript variant 1 |
CNY 8,632.00 |
