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SOX9 (NM_000346) Human Tagged ORF Clone
CAT＃: RC208944
- TrueORF®
SOX9 (Myc-DDK-tagged)-Human SRY (sex determining region Y)-box 9 (SOX9)
ORF Plasmid: tGFP
"NM_000346" in other vectors (6)
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CNY 5,688.00
| Cited in 11 publications. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CMD1; CMPD1; SRA1; SRXX2; SRXY10 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208944 representing NM_000346
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATCTCCTGGACCCCTTCATGAAGATGACCGACGAGCAGGAGAAGGGCCTGTCCGGCGCCCCCAGCC CCACCATGTCCGAGGACTCCGCGGGCTCGCCCTGCCCGTCGGGCTCCGGCTCGGACACCGAGAACACGCG GCCCCAGGAGAACACGTTCCCCAAGGGCGAGCCCGATCTGAAGAAGGAGAGCGAGGAGGACAAGTTCCCC GTGTGCATCCGCGAGGCGGTCAGCCAGGTGCTCAAAGGCTACGACTGGACGCTGGTGCCCATGCCGGTGC GCGTCAACGGCTCCAGCAAGAACAAGCCGCACGTCAAGCGGCCCATGAACGCCTTCATGGTGTGGGCGCA GGCGGCGCGCAGGAAGCTCGCGGACCAGTACCCGCACTTGCACAACGCCGAGCTCAGCAAGACGCTGGGC AAGCTCTGGAGACTTCTGAACGAGAGCGAGAAGCGGCCCTTCGTGGAGGAGGCGGAGCGGCTGCGCGTGC AGCACAAGAAGGACCACCCGGATTACAAGTACCAGCCGCGGCGGAGGAAGTCGGTGAAGAACGGGCAGGC GGAGGCAGAGGAGGCCACGGAGCAGACGCACATCTCCCCCAACGCCATCTTCAAGGCGCTGCAGGCCGAC TCGCCACACTCCTCCTCCGGCATGAGCGAGGTGCACTCCCCCGGCGAGCACTCGGGGCAATCCCAGGGCC CACCGACCCCACCCACCACCCCCAAAACCGACGTGCAGCCGGGCAAGGCTGACCTGAAGCGAGAGGGGCG CCCCTTGCCAGAGGGGGGCAGACAGCCCCCTATCGACTTCCGCGACGTGGACATCGGCGAGCTGAGCAGC GACGTCATCTCCAACATCGAGACCTTCGATGTCAACGAGTTTGACCAGTACCTGCCGCCCAACGGCCACC CGGGGGTGCCGGCCACGCACGGCCAGGTCACCTACACGGGCAGCTACGGCATCAGCAGCACCGCGGCCAC CCCGGCGAGCGCGGGCCACGTGTGGATGTCCAAGCAGCAGGCGCCGCCGCCACCCCCGCAGCAGCCCCCA CAGGCCCCGCCGGCCCCGCAGGCGCCCCCGCAGCCGCAGGCGGCGCCCCCACAGCAGCCGGCGGCACCCC CGCAGCAGCCACAGGCGCACACGCTGACCACGCTGAGCAGCGAGCCGGGCCAGTCCCAGCGAACGCACAT CAAGACGGAGCAGCTGAGCCCCAGCCACTACAGCGAGCAGCAGCAGCACTCGCCCCAACAGATCGCCTAC AGCCCCTTCAACCTCCCACACTACAGCCCCTCCTACCCGCCCATCACCCGCTCACAGTACGACTACACCG ACCACCAGAACTCCAGCTCCTACTACAGCCACGCGGCAGGCCAGGGCACCGGCCTCTACTCCACCTTCAC CTACATGAACCCCGCTCAGCGCCCCATGTACACCCCCATCGCCGACACCTCTGGGGTCCCTTCCATCCCG CAGACCCACAGCCCCCAGCACTGGGAACAACCCGTCTACACACAGCTCACTCGACCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208944 representing NM_000346
Red=Cloning site Green=Tags(s) MNLLDPFMKMTDEQEKGLSGAPSPTMSEDSAGSPCPSGSGSDTENTRPQENTFPKGEPDLKKESEEDKFP VCIREAVSQVLKGYDWTLVPMPVRVNGSSKNKPHVKRPMNAFMVWAQAARRKLADQYPHLHNAELSKTLG KLWRLLNESEKRPFVEEAERLRVQHKKDHPDYKYQPRRRKSVKNGQAEAEEATEQTHISPNAIFKALQAD SPHSSSGMSEVHSPGEHSGQSQGPPTPPTTPKTDVQPGKADLKREGRPLPEGGRQPPIDFRDVDIGELSS DVISNIETFDVNEFDQYLPPNGHPGVPATHGQVTYTGSYGISSTAATPASAGHVWMSKQQAPPPPPQQPP QAPPAPQAPPQPQAAPPQQPAAPPQQPQAHTLTTLSSEPGQSQRTHIKTEQLSPSHYSEQQQHSPQQIAY SPFNLPHYSPSYPPITRSQYDYTDHQNSSSYYSHAAGQGTGLYSTFTYMNPAQRPMYTPIADTSGVPSIP QTHSPQHWEQPVYTQLTRP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000346 |
| ORF Size | 1527 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000346.4 |
| RefSeq Size | 3935 bp |
| RefSeq ORF | 1530 bp |
| Locus ID | 6662 |
| UniProt ID | P48436 |
| Domains | HMG |
| Protein Families | Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors |
| MW | 56 kDa |
| Gene Summary | The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal. [provided by RefSeq, Jul 2008] |
Citations (11)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Repression of Wnt/β-catenin signaling by SOX9 and Mastermind-like transcriptional coactivator 2
,Sinha, A;Fan, VB;Ramakrishnan, AB;Engelhardt, N;Kennell, J;Cadigan, KM;,
Science advances
,PubMed ID 33597243
[SOX9]
|
|
A retrotransposon gag-like-3 gene RTL3 and SOX-9 co-regulate the expression of COL2A1 in chondrocytes.
,null,
Connective tissue research
,PubMed ID 33043724
[SOX9]
|
|
SOX9 promotes stress-responsive transcription of VGF nerve growth factor inducible gene in renal tubular epithelial cells
,Kim, JY;Bai, Y;Jayne, LA;Abdulkader, F;Gandhi, M;Perreau, T;Parikh, SV;Gardner, DS;Davidson, AJ;Sander, V;Song, MA;Bajwa, A;Pabla, NS;,
J. Biol. Chem.
,PubMed ID 32887795
[SOX9]
|
|
SOX9 promotes stress-responsive transcription of VGF nerve growth factor inducible gene in kidney epithelial cells
,Kim, J;Bai, Y;Jayne, L;Abdulkader, F;Gandhi, M;Parikh, S;Song, M;Bajwa, A;Pabla, N;,
bioRxiv
[SOX9]
|
|
Changes in Fluorescence Recovery After Photobleaching (FRAP) as an indicator of SOX9 transcription factor activity
,Govindaraj, K;Hendriks, J;Lidke, DS;Karperien, M;Post, JN;,
Biochim Biophys Acta Gene Regul Mech
,PubMed ID 30465885
[SOX9]
|
|
Odd-skipped related 1 inhibits lung cancer proliferation and invasion by reducing Wnt signaling through the suppression of SOX9 and β-catenin
,Wang, Y;Lei, L;Zheng, YW;Zhang, L;Li, ZH;Shen, HY;Jiang, GY;Zhang, XP;Wang, EH;Xu, HT;,
Cancer Sci.
,PubMed ID 29660200
[SOX9]
|
|
Comprehensive characterisation of compartment-specific long non-coding RNAs associated with pancreatic ductal adenocarcinoma
,Arnes, L;Liu, Z;Wang, J;Carlo Maurer, H;Sagalovskiy, I;Sanchez-Martin, M;Bommakanti, N;Garofalo, DC;Balderes, DA;Sussel, L;Olive, KP;Rabadan, R;,
Gut
,PubMed ID 29440233
[SOX9]
|
|
Sox9 confers stemness properties in hepatocellular carcinoma through Frizzled-7 mediated Wnt/β-catenin signaling
,null,
Oncotarget
,PubMed ID 27105493
[SOX9]
|
|
Testicular dysgenesis/regression without campomelic dysplasia in patients carrying missense mutations and upstream deletion of SOX9
,Katoh-Fukui, Y;Igarashi, M;Nagasaki, K;,
Molecular Genetics & Genomic Medicine
[SOX9]
|
|
Testicular dysgenesis/regression without campomelic dysplasia in patients carrying missense mutations and upstream deletion of SOX9
,null,
Molecular Genetics & Genomic Medicine
,PubMed ID 26740947
[SOX9]
|
|
Regulation of the Bone-restricted IFITM-like ( Bril ) Gene Transcription by Sp and Gli Family Members and CpG Methylation
,null,
The Journal of Biological Chemistry
,PubMed ID 23530031
[SOX9]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208944L1 | Lenti-ORF clone of SOX9 (Myc-DDK-tagged)-Human SRY (sex determining region Y)-box 9 (SOX9) |
CNY 8,088.00 |
|
| RC208944L2 | Lenti-ORF clone of SOX9 (mGFP-tagged)-Human SRY (sex determining region Y)-box 9 (SOX9) |
CNY 6,460.00 |
|
| RC208944L3 | Lenti-ORF clone of SOX9 (Myc-DDK-tagged)-Human SRY (sex determining region Y)-box 9 (SOX9) |
CNY 8,088.00 |
|
| RC208944L4 | Lenti-ORF clone of SOX9 (mGFP-tagged)-Human SRY (sex determining region Y)-box 9 (SOX9) |
CNY 8,088.00 |
|
| RG208944 | SOX9 (tGFP-tagged) - Human SRY (sex determining region Y)-box 9 (SOX9) |
CNY 7,288.00 |
|
| SC321884 | SOX9 (untagged)-Human SRY (sex determining region Y)-box 9 (SOX9) |
CNY 3,800.00 |
