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CENPB (NM_001810) Human Tagged ORF Clone
CAT＃: RC208842
- TrueORF®
CENPB (Myc-DDK-tagged)-Human centromere protein B, 80kDa (CENPB)
ORF Plasmid: tGFP
"NM_001810" in other vectors (4)
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CNY 6,688.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208842 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCCCAAGAGGCGACAGCTGACGTTCCGGGAGAAGTCACGGATCATCCAGGAGGTGGAGGAGAATC CGGACCTGCGCAAGGGCGAGATCGCGCGGCGCTTCAACATCCCGCCGTCCACGCTGAGCACGATCCTGAA GAACAAGCGCGCCATCCTGGCGTCGGAGCGCAAGTACGGGGTGGCCTCCACCTGCCGCAAGACCAACAAG CTGTCTCCCTACGACAAGCTCGAGGGCTTGCTCATCGCCTGGTTCCAGCAGATCCGCGCCGCCGGCCTGC CGGTCAAGGGCATCATCCTCAAGGAGAAGGCGCTGCGCATAGCCGAGGAGCTGGGCATGGACGACTTCAC CGCCTCCAACGGCTGGCTGGACCGCTTCCGCCGGCGCCACGGCGTGGTGTCCTGCAGCGGCGTGGCCCGC GCCCGCGCGCGAAACGCTGCCCCCCGCACCCCGGCGGCGCCTGCCAGTCCGGCCGCGGTGCCCTCGGAGG GCAGTGGCGGGAGCACTACTGGTTGGCGCGCTCGGGAGGAGCAGCCGCCGTCGGTGGCCGAGGGCTACGC CTCGCAGGACGTGTTCAGCGCCACCGAGACCAGTCTATGGTACGACTTCCTGCCCGACCAGGCCGCGGGG CTGTGCGGAGGCGACGGACGGCCGCGTCAAGCCACCCAGCGCCTGAGCGTCCTGCTATGCGCCAATGCCG ACGGCAGCGAGAAGCTGCCCCCGCTGGTGGCCGGCAAGTCGGCCAAGCCCCGCGCAGGCCAAGCCGGCCT GCCCTGCGACTACACCGCCAACTCCAAGGGTGGTGTCACCACCCAGGCCCTGGCCAAGTACTTGAAGGCC TTGGACACCCGAATGGCTGCAGAGTCTCGCCGGGTCCTGCTGTTGGCCGGCCGCTTGGCTGCCCAGTCCT TGGACACCTCGGGCCTGCGGCATGTGCAGCTGGCCTTCTTCCCTCCCGGCACCGTGCATCCGCTGGAGAG GGGAGTGGTCCAGCAGGTGAAGGGCCACTACCGCCAGGCCATGCTGCTCAAGGCCATGGCCGCGCTAGAG GGCCAGGATCCCTCAGGCCTGCAGCTGGGTCTCACGGAGGCCCTGCACTTTGTGGCTGCCGCCTGGCAGG CAGTGGAGCCTTCGGACATAGCCGCCTGCTTTCGTGAGGCTGGCTTTGGGGGTGGCCCTAATGCCACCAT CACCACTTCCCTCAAGAGTGAGGGAGAGGAAGAGGAGGAGGAGGAGGAAGAAGAGGAGGAGGAAGAGGGT GAAGGAGAGGAAGAGGAGGAGGAAGGGGAGGAGGAGGAGGAGGAAGGGGGGGAAGGAGAGGAATTGGGGG AGGAAGAGGAGGTGGAGGAGGAGGGTGATGTTGATAGTGATGAAGAAGAGGAGGAAGATGAGGAGAGCTC CTCGGAGGGCTTGGAGGCTGAGGACTGGGCCCAGGGAGTAGTGGAGGCCGGTGGCAGCTTCGGGGCTTAT GGTGCCCAGGAGGAAGCCCAGTGCCCTACTCTGCATTTCCTGGAAGGTGGGGAGGACTCTGATTCAGACA GTGAGGAAGAGGACGATGAGGAAGAGGATGATGAAGATGAAGACGACGATGATGATGAGGAGGATGGTGA TGAGGTGCCTGTACCCAGCTTTGGGGAGGCCATGGCTTACTTTGCCATGGTCAAGAGGTACCTGACCTCC TTCCCCATTGATGACCGCGTGCAGAGCCACATCCTCCACTTGGAACACGATCTGGTTCATGTGACCAGGA AGAACCACGCCAGGCAGGCGGGAGTTCGAGGTCTTGGACATCAAAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208842 protein sequence
Red=Cloning site Green=Tags(s) MGPKRRQLTFREKSRIIQEVEENPDLRKGEIARRFNIPPSTLSTILKNKRAILASERKYGVASTCRKTNK LSPYDKLEGLLIAWFQQIRAAGLPVKGIILKEKALRIAEELGMDDFTASNGWLDRFRRRHGVVSCSGVAR ARARNAAPRTPAAPASPAAVPSEGSGGSTTGWRAREEQPPSVAEGYASQDVFSATETSLWYDFLPDQAAG LCGGDGRPRQATQRLSVLLCANADGSEKLPPLVAGKSAKPRAGQAGLPCDYTANSKGGVTTQALAKYLKA LDTRMAAESRRVLLLAGRLAAQSLDTSGLRHVQLAFFPPGTVHPLERGVVQQVKGHYRQAMLLKAMAALE GQDPSGLQLGLTEALHFVAAAWQAVEPSDIAACFREAGFGGGPNATITTSLKSEGEEEEEEEEEEEEEEG EGEEEEEEGEEEEEEGGEGEELGEEEEVEEEGDVDSDEEEEEDEESSSEGLEAEDWAQGVVEAGGSFGAY GAQEEAQCPTLHFLEGGEDSDSDSEEEDDEEEDDEDEDDDDDEEDGDEVPVPSFGEAMAYFAMVKRYLTS FPIDDRVQSHILHLEHDLVHVTRKNHARQAGVRGLGHQS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001810 |
| ORF Size | 1797 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001810.6 |
| RefSeq Size | 2856 bp |
| RefSeq ORF | 1800 bp |
| Locus ID | 1059 |
| UniProt ID | P07199 |
| Protein Families | Druggable Genome |
| MW | 65.2 kDa |
| Gene Summary | This gene product is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208842L3 | Lenti-ORF clone of CENPB (Myc-DDK-tagged)-Human centromere protein B, 80kDa (CENPB) |
CNY 5,890.00 |
|
| RC208842L4 | Lenti-ORF clone of CENPB (mGFP-tagged)-Human centromere protein B, 80kDa (CENPB) |
CNY 9,088.00 |
|
| RG208842 | CENPB (tGFP-tagged) - Human centromere protein B, 80kDa (CENPB) |
CNY 4,370.00 |
|
| SC303073 | CENPB (untagged)-Human centromere protein B, 80kDa (CENPB) |
CNY 7,220.00 |
