United States
Germany
Japan
United Kingdom
China
NADPH oxidase 4 (NOX4) (NM_016931) Human Tagged ORF Clone
CAT＃: RC208007
- TrueORF®
NOX4 (Myc-DDK-tagged)-Human NADPH oxidase 4 (NOX4), transcript variant 1
ORF Plasmid: tGFP
"NM_016931" in other vectors (7)
Need custom modification / cloning service?
Get a free quote
CNY 4,304.00
CNY 4,560.00
| Cited in 9 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | KOX; KOX-1; RENOX |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208007 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGTGTCCTGGAGGAGCTGGCTCGCCAACGAAGGGGTTAAACACCTCTGCCTGTTCATCTGGCTCT CCATGAATGTCCTGCTTTTCTGGAAAACCTTCTTGCTGTATAACCAAGGGCCAGAGTATCACTACCTCCA CCAGATGTTGGGGCTAGGATTGTGTCTAAGCAGAGCCTCAGCATCTGTTCTTAACCTCAACTGCAGCCTT ATCCTTTTACCCATGTGCCGAACACTCTTGGCTTACCTCCGAGGATCACAGAAGGTTCCAAGCAGGAGAA CCAGGAGATTGTTGGATAAAAGCAGAACATTCCATATTACCTGTGGTGTTACTATCTGTATTTTCTCAGG CGTGCATGTGGCTGCCCATCTGGTGAATGCCCTCAACTTCTCAGTGAATTACAGTGAAGACTTTGTTGAA CTGAATGCAGCAAGATACCGAGATGAGGATCCTAGAAAACTTCTCTTCACAACTGTTCCTGGCCTGACAG GGGTCTGCATGGTGGTGGTGCTATTCCTCATGATCACAGCCTCTACATATGCAATAAGAGTTTCTAACTA TGATATCTTCTGGTATACTCATAACCTCTTCTTTGTCTTCTACATGCTGCTGACGTTGCATGTTTCAGGA GGGCTGCTGAAGTATCAAACTAATTTAGATACCCACCCTCCCGGCTGCATCAGTCTTAACCGAACCAGCT CTCAGAATATTTCCTTACCAGAGTATTTCTCAGAACATTTTCATGAACCTTTCCCTGAAGGATTTTCAAA ACCGGCAGAGTTTACCCAGCACAAATTTGTGAAGATTTGTATGGAAGAGCCCAGATTCCAAGCTAATTTT CCACAGACTTGGCTTTGGATTTCTGGACCTTTGTGCCTGTACTGTGCCGAAAGACTTTACAGGTATATCC GGAGCAATAAGCCAGTCACCATCATTTCGGTCATAAGTCATCCCTCAGATGTCATGGAAATCCGAATGGT CAAAGAAAATTTTAAAGCAAGACCTGGTCAGTATATTACTCTACATTGTCCCAGTGTATCTGCATTAGAA AATCATCCATTTACCCTCACAATGTGTCCAACTGAAACCAAAGCAACATTTGGGGTTCATCTTAAAATAG TAGGAGACTGGACAGAACGATTTCGAGATTTACTACTGCCTCCATCTAGTCAAGACTCCGAAATTCTGCC CTTCATTCAATCTAGAAATTATCCCAAGCTGTATATTGATGGTCCTTTTGGAAGTCCATTTGAGGAATCA CTGAACTATGAGGTCAGCCTCTGCGTGGCTGGAGGCATTGGAGTAACTCCATTTGCATCAATACTCAACA CCCTGTTGGATGACTGGAAACCATACAAGCTTAGAAGACTATACTTTATTTGGGTATGCAGAGATATCCA GTCCTTCCGTTGGTTTGCAGATTTACTCTGTATGTTGCATAACAAGTTTTGGCAAGAGAACAGACCTGAC TATGTCAACATCCAGCTGTACCTCAGTCAAACAGATGGGATACAGAAGATAATTGGAGAAAAATATCATG CACTGAATTCAAGACTGTTTATAGGACGTCCTCGGTGGAAACTTTTGTTTGATGAAATAGCAAAATATAA CAGAGGAAAAACAGTTGGTGTTTTCTGTTGTGGACCCAATTCACTATCCAAGACTCTTCATAAACTGAGT AACCAGAACAACTCATATGGGACAAGATTTGAATACAATAAAGAGTCTTTCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208007 protein sequence
Red=Cloning site Green=Tags(s) MAVSWRSWLANEGVKHLCLFIWLSMNVLLFWKTFLLYNQGPEYHYLHQMLGLGLCLSRASASVLNLNCSL ILLPMCRTLLAYLRGSQKVPSRRTRRLLDKSRTFHITCGVTICIFSGVHVAAHLVNALNFSVNYSEDFVE LNAARYRDEDPRKLLFTTVPGLTGVCMVVVLFLMITASTYAIRVSNYDIFWYTHNLFFVFYMLLTLHVSG GLLKYQTNLDTHPPGCISLNRTSSQNISLPEYFSEHFHEPFPEGFSKPAEFTQHKFVKICMEEPRFQANF PQTWLWISGPLCLYCAERLYRYIRSNKPVTIISVISHPSDVMEIRMVKENFKARPGQYITLHCPSVSALE NHPFTLTMCPTETKATFGVHLKIVGDWTERFRDLLLPPSSQDSEILPFIQSRNYPKLYIDGPFGSPFEES LNYEVSLCVAGGIGVTPFASILNTLLDDWKPYKLRRLYFIWVCRDIQSFRWFADLLCMLHNKFWQENRPD YVNIQLYLSQTDGIQKIIGEKYHALNSRLFIGRPRWKLLFDEIAKYNRGKTVGVFCCGPNSLSKTLHKLS NQNNSYGTRFEYNKESFS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016931 |
| ORF Size | 1734 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016931.4 |
| RefSeq Size | 4459 bp |
| RefSeq ORF | 1737 bp |
| Locus ID | 50507 |
| UniProt ID | Q9NPH5 |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 66.9 kDa |
| Gene Summary | This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2009] |
Citations (9)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Hypoxia promotes the metastasis of pancreatic cancer through regulating NOX4/KDM5A-mediated histone methylation modification changes in a HIF1A-independent manner
,Li, H;Peng, C;Zhu, C;Nie, S;Qian, X;Shi, Z;Shi, M;Liang, Y;Ding, X;Zhang, S;Zhang, B;Li, X;Xu, G;Lv, Y;Wang, L;Friess, H;Kong, B;Zou, X;Shen, S;,
Clinical epigenetics
,PubMed ID 33499904
[NADPH oxidase 4]
|
|
NADPH Oxidase 4 Promotes Hypoxia-induced Epithelial-to-mesenchymal Transition via Histone Modification in Pancreatic cancer
,Li, H;Peng, C;Zhu, C;Nie, S;Qian, X;Shi, Z;Shi, M;Liang, Y;Ding, X;Zhang, S;Zhang, B;Li, X;Xu, G;Lv, Y;Wang, L;Friess, H;Kong, B;Zou, X;Shen, S;,
Research Square
[NADPH oxidase 4]
|
|
NADPH oxidase 1 is highly expressed in human large and small bowel cancers
,Lu, J;Jiang, G;Wu, Y;Antony, S;Meitzler, JL;Juhasz, A;Liu, H;Roy, K;Makhlouf, H;Chuaqui, R;Butcher, D;Konaté, MM;Doroshow, JH;,
PLoS ONE
,PubMed ID 32428030
[NADPH oxidase 4]
|
|
Anagliptin prevents apoptosis of human umbilical vein endothelial cells by modulating NOX-4 signaling pathways
,Li, Q;Li, J;Liu, Y;Zhang, M;Chen, C;,
Biomedicine & Pharmacotherapy
,PubMed ID 29864951
[NADPH oxidase 4]
|
|
HAX-1 regulates SERCA2a oxidation and degradation
,Bidwell, PA;Liu, GS;Nagarajan, N;Lam, CK;Haghighi, K;Gardner, G;Cai, WF;Zhao, W;Mugge, L;Vafiadaki, E;Sanoudou, D;Rubinstein, J;Lebeche, D;Hajjar, R;Sadoshima, J;Kranias, EG;,
J. Mol. Cell. Cardiol.2017
,PubMed ID 29169992
[NADPH oxidase 4]
|
|
Decoding NADPH Oxidase 4 Expression in Human Tumors
,Meitzler, JL;Makhlouf, HR;Antony, S;Wu, Y;Butcher, D;Jianga, G;Juhasz, A;Lu, J;Dahan, I;Jansen-Dürr, P;Pircher, H;Shah, AM;,
Redox Biology
,PubMed ID 28578276
[NADPH oxidase 4]
|
|
NOX isoforms in the development of abdominal aortic aneurysm
,Siu, KL;Li, Q;Zhang, Y;Guo, J;Youn, JY;Du, J;Cai, H;,
Redox Biol
,PubMed ID 27912196
[NADPH oxidase 4]
|
|
mTORC2 Coordinates Pulmonary Artery Smooth Muscle Cell Metabolism, Proliferation and Survival in Pulmonary Arterial Hypertension
,Goncharov, DA;Kudryashova, TV;Ziai, H;Ihida-Stansbury, K;Delisser, H;Krymskaya, VP;Tuder, RM;Kawut, SM;Goncharova, EA;,
Circulation
,PubMed ID 24273214
[NADPH oxidase 4]
|
|
Characterization of NADPH oxidase 5 expression in human tumors and tumor cell lines with a novel mouse monoclonal antibody
,null,
Free radical biology & medicine
,PubMed ID 23851018
[NADPH oxidase 4]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208007L1 | Lenti ORF clone of Human NADPH oxidase 4 (NOX4), transcript variant 1, Myc-DDK-tagged |
CNY 6,704.00 |
|
| RC208007L2 | Lenti ORF clone of Human NADPH oxidase 4 (NOX4), transcript variant 1, mGFP tagged |
CNY 6,704.00 |
|
| RC208007L3 | Lenti ORF clone of Human NADPH oxidase 4 (NOX4), transcript variant 1, Myc-DDK-tagged |
CNY 6,704.00 |
|
| RC208007L4 | Lenti ORF clone of Human NADPH oxidase 4 (NOX4), transcript variant 1, mGFP tagged |
CNY 6,704.00 |
|
| RG208007 | NOX4 (tGFP-tagged) - Human NADPH oxidase 4 (NOX4), transcript variant 1 |
CNY 5,904.00 |
|
| SC310253 | NOX4 (untagged)-Human NADPH oxidase 4 (NOX4), transcript variant 1 |
CNY 6,464.00 |
|
| SC322623 | NOX4 (untagged)-Human NADPH oxidase 4 (NOX4), transcript variant 1 |
CNY 6,464.00 |
其它NADPH oxidase 4产品
