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RFX1 (NM_002918) Human Tagged ORF Clone
CAT＃: RC207872
RFX1 (Myc-DDK-tagged)-Human regulatory factor X, 1 (influences HLA class II expression) (RFX1)
ORF Plasmid: tGFP
"NM_002918" in other vectors (6)
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CNY 7,130.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EFC; RFX |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207872 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAACACAGGCGTATACTGAGCTACAGGCAGCCCCGCCACCATCCCAGCCGCCACAGGCCCCGCCAC AAGCCCAGCCCCAGCCGCCACCGCCACCACCCCCAGCGGCACCCCAGCCCCCGCAGCCACCCACCGCTGC TGCCACCCCTCAGCCCCAATATGTCACCGAGCTGCAGAGCCCCCAGCCCCAGGCACAGCCACCGGGTGGC CAGAAGCAGTACGTGACGGAGCTCCCGGCTGTACCCGCACCCTCGCAGCCAACCGGTGCACCCACCCCTT CGCCTGCACCCCAGCAGTACATCGTGGTCACTGTCTCTGAAGGTGCCATGCGGGCCAGCGAGACAGTGTC GGAGGCCAGCCCCGGCTCCACCGCCAGCCAGACCGGCGTTCCTACTCAGGTGGTTCAGCAGGTGCAGGGC ACCCAGCAGCGGCTGCTGGTCCAGACGAGCGTGCAGGCCAAGCCAGGCCACGTGTCGCCCCTCCAGCTGA CCAACATCCAAGTGCCCCAGCAGGCTCTTCCCACGCAGCGTCTGGTGGTGCAGAGCGCAGCCCCAGGCAG CAAAGGTGGCCAGGTCTCCCTGACGGTCCATGGTACCCAGCAGGTGCACTCGCCCCCAGAGCAGTCGCCG GTGCAGGCCAACAGCTCTTCCAGCAAGACAGCCGGGGCCCCCACGGGCACAGTGCCACAGCAGCTGCAGG TCCACGGCGTCCAGCAGAGTGTCCCCGTCACCCAAGAGAGATCTGTGGTCCAGGCCACTCCACAAGCGCC CAAACCCGGCCCGGTGCAGCCGCTGACCGTGCAGGGCCTCCAGCCAGTCCACGTGGCTCAAGAGGTGCAG CAGCTCCAGCAGGTGCCCGTCCCACACGTGTACTCCAGCCAGGTGCAGTATGTGGAGGGCGGCGATGCCA GCTACACGGCCAGTGCCATCCGTTCCAGCACCTACTCCTATCCCGAGACGCCGCTGTACACGCAGACGGC AAGCACCAGCTACTACGAGGCCGCAGGCACGGCCACCCAGGTCAGCACCCCCGCCACCTCCCAGGCGGTG GCCAGCAGTGGCTCCATGCCCATGTACGTGTCCGGCAGCCAGGTCGTCGCCAGCTCCGCCAGCACTGGGG CTGGGGCCAGCAACAGCAGCGGAGGTGGTGGCAGTGGTGGTGGCGGCGGCGGCGGGGGAGGCGGTGGCGG GGGTGGCAGTGGCAGCACCGGAGGCGGCGGCAGCGGAGCAGGCACCTACGTGATCCAAGGCGGCTACATG CTGGGCAGTGCCAGCCAGTCTTACTCTCACACCACCCGTGCCTCGCCAGCCACGGTCCAGTGGCTCCTGG ACAACTATGAGACGGCTGAGGGCGTGAGTCTGCCACGGAGCACCCTCTACTGCCACTACTTACTGCACTG CCAGGAGCAGAAGCTGGAGCCCGTCAACGCCGCCTCCTTCGGCAAGCTCATCCGCTCCGTCTTCATGGGC CTGCGAACCCGCCGTCTGGGCACCAGGGGCAACTCCAAGTACCACTACTATGGCCTGCGCATCAAGGCCA GCTCACCCCTGCTGCGGCTGATGGAGGACCAGCAGCACATGGCCATGCGGGGCCAGCCCTTCTCGCAGAA GCAGAGGCTCAAGCCCATCCAGAAGATGGAAGGCATGACCAACGGCGTGGCGGTGGGGCAGCAGCCGAGC ACGGGGCTGTCGGACATCAGCGCCCAGGTGCAGCAGTACCAGCAATTTTTGGATGCCTCTCGGAGCCTCC CTGACTTCACAGAGCTCGACCTCCAGGGCAAGGTGCTGCCTGAGGGCGTCGGGCCCGGGGACATCAAAGC CTTCCAGGTCCTGTACCGGGAACACTGTGAGGCCATTGTCGACGTCATGGTGAACCTGCAGTTCACCCTG GTGGAGACGCTGTGGAAGACCTTCTGGAGGTACAACCTCAGCCAGCCCAGTGAGGCGCCACCGCTGGCTG TACATGACGAGGCCGAGAAGCGACTGCCCAAAGCCATCCTGGTGCTCCTCTCCAAGTTCGAGCCCGTGCT CCAATGGACCAAGCACTGTGACAACGTGCTGTACCAGGGCCTGGTGGAAATCCTCATTCCCGACGTGCTG CGGCCCATCCCCAGTGCCTTGACCCAAGCGATCCGGAACTTTGCCAAGAGCCTGGAGAGCTGGCTCACCC ACGCCATGGTCAACATCCCCGAGGAGATGCTGCGGGTGAAGGTGGCCGCGGCTGGCGCCTTCGCGCAGAC ACTGCGGCGCTACACGTCGCTCAACCACCTGGCGCAGGCGGCGCGCGCTGTGCTGCAGAACACCGCACAG ATCAACCAGATGCTGAGCGACCTCAACCGCGTGGACTTCGCCAACGTGCAGGAGCAGGCCTCGTGGGTGT GCCGCTGCGAGGACCGCGTGGTGCAGCGGCTGGAGCAGGACTTCAAGGTGACGCTGCAGCAGCAGAACTC GCTGGAGCAGTGGGCGGCCTGGCTGGACGGCGTGGTGAGCCAGGTGCTCAAGCCCTACCAGGGCAGCGCC GGCTTCCCCAAGGCCGCCAAGCTCTTCCTCCTCAAGTGGTCCTTCTACAGCTCCATGGTGATCCGGGACC TGACCCTGCGCAGCGCCGCCAGCTTCGGTTCCTTCCACCTCATCCGGCTGCTCTACGACGAGTACATGTA CTACCTGATCGAGCACCGCGTAGCCCAGGCCAAGGGCGAGACCCCCATCGCCGTCATGGGCGAGTTCGCC AATCTGGCCACCTCCCTGAACCCCCTGGACCCCGACAAAGACGAGGAGGAAGAAGAGGAGGAGGAGAGCG AGGACGAGCTGCCGCAGGACATCTCACTGGCGGCTGGCGGCGAGTCACCCGCGCTGGGCCCGGAGACCCT GGAGCCGCCGGCCAAGCTGGCGCGGACTGACGCGCGCGGCCTCTTCGTGCAGGCGCTGCCCTCCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207872 protein sequence
Red=Cloning site Green=Tags(s) MATQAYTELQAAPPPSQPPQAPPQAQPQPPPPPPPAAPQPPQPPTAAATPQPQYVTELQSPQPQAQPPGG QKQYVTELPAVPAPSQPTGAPTPSPAPQQYIVVTVSEGAMRASETVSEASPGSTASQTGVPTQVVQQVQG TQQRLLVQTSVQAKPGHVSPLQLTNIQVPQQALPTQRLVVQSAAPGSKGGQVSLTVHGTQQVHSPPEQSP VQANSSSSKTAGAPTGTVPQQLQVHGVQQSVPVTQERSVVQATPQAPKPGPVQPLTVQGLQPVHVAQEVQ QLQQVPVPHVYSSQVQYVEGGDASYTASAIRSSTYSYPETPLYTQTASTSYYEAAGTATQVSTPATSQAV ASSGSMPMYVSGSQVVASSASTGAGASNSSGGGGSGGGGGGGGGGGGGGSGSTGGGGSGAGTYVIQGGYM LGSASQSYSHTTRASPATVQWLLDNYETAEGVSLPRSTLYCHYLLHCQEQKLEPVNAASFGKLIRSVFMG LRTRRLGTRGNSKYHYYGLRIKASSPLLRLMEDQQHMAMRGQPFSQKQRLKPIQKMEGMTNGVAVGQQPS TGLSDISAQVQQYQQFLDASRSLPDFTELDLQGKVLPEGVGPGDIKAFQVLYREHCEAIVDVMVNLQFTL VETLWKTFWRYNLSQPSEAPPLAVHDEAEKRLPKAILVLLSKFEPVLQWTKHCDNVLYQGLVEILIPDVL RPIPSALTQAIRNFAKSLESWLTHAMVNIPEEMLRVKVAAAGAFAQTLRRYTSLNHLAQAARAVLQNTAQ INQMLSDLNRVDFANVQEQASWVCRCEDRVVQRLEQDFKVTLQQQNSLEQWAAWLDGVVSQVLKPYQGSA GFPKAAKLFLLKWSFYSSMVIRDLTLRSAASFGSFHLIRLLYDEYMYYLIEHRVAQAKGETPIAVMGEFA NLATSLNPLDPDKDEEEEEEEESEDELPQDISLAAGGESPALGPETLEPPAKLARTDARGLFVQALPSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002918 |
| ORF Size | 2937 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002918.3, NP_002909.3 |
| RefSeq Size | 4392 bp |
| RefSeq ORF | 2940 bp |
| Locus ID | 5989 |
| UniProt ID | P22670 |
| Protein Families | Druggable Genome |
| MW | 104.7 kDa |
| Gene Summary | This gene encodes a member of the regulatory factor X (RFX) family of transcription factors, which are characterized by a winged-helix DNA-binding domain. The encoded transcription factor contains an N-terminal activation domain and a C-terminal repression domain, and may activate or repress target gene expression depending on cellular context. This transcription factor has been shown to regulate a wide variety of genes involved in immunity and cancer, including the MHC class II genes and genes that may be involved in cancer progression. This gene exhibits altered expression in glioblastoma and the autoimmune disease systemic lupus erythematosis (SLE). [provided by RefSeq, Jul 2016] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The G1613A Mutation in the HBV Genome Affects HBeAg Expression and Viral Replication through Altered Core Promoter Activity
,null,
PLoS ONE
,PubMed ID 21814558
[RFX1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207872L1 | Lenti ORF clone of Human regulatory factor X, 1 (influences HLA class II expression) (RFX1), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC207872L2 | Lenti ORF clone of Human regulatory factor X, 1 (influences HLA class II expression) (RFX1), mGFP tagged |
CNY 9,030.00 |
|
| RC207872L3 | Lenti ORF clone of Human regulatory factor X, 1 (influences HLA class II expression) (RFX1), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC207872L4 | Lenti ORF clone of Human regulatory factor X, 1 (influences HLA class II expression) (RFX1), mGFP tagged |
CNY 9,030.00 |
|
| RG207872 | RFX1 (tGFP-tagged) - Human regulatory factor X, 1 (influences HLA class II expression) (RFX1) |
CNY 7,890.00 |
|
| SC324081 | RFX1 (untagged)-Human regulatory factor X, 1 (influences HLA class II expression) (RFX1) |
CNY 7,176.00 |
