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NT5M (NM_020201) Human Tagged ORF Clone
CAT＃: RC207737
NT5M (Myc-DDK-tagged)-Human 5',3'-nucleotidase, mitochondrial (NT5M), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_020201" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | dNT-2; dNT2; mdN |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207737 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCCGGCTGGGCGGCTGGTGTGCGCGGCGGCTCTGCAGCGCGGCGGTTCCCGCGGGGCGGCGCGGGG CGGCGGGCGGGCTGGGCCTGGCGGGAGGCCGCGCCCTACGGGTGCTGGTGGACATGGACGGCGTGCTGGC TGACTTCGAGGGCGGATTCCTCAGGAAGTTCCGCGCGCGCTTTCCCGACCAGCCCTTCATCGCGCTGGAG GACCGGCGCGGCTTCTGGGTGTCGGAGCAGTACGGCCGCCTGCGGCCAGGGCTGAGCGAGAAGGCCATCA GCATTTGGGAGTCAAAGAATTTCTTTTTTGAACTTGAGCCTCTGCCAGGGGCCGTGGAAGCTGTCAAGGA GATGGCCAGCCTACAAAACACTGACGTCTTCATCTGCACAAGCCCCATCAAGATGTTCAAGTACTGTCCC TATGAGAAGTATGCCTGGGTGGAGAAGTACTTTGGCCCTGACTTTCTGGAGCAGATTGTGCTGACCAGAG ACAAGACCGTGGTCTCTGCTGACCTTCTCATAGACGACCGGCCGGACATCACAGGGGCCGAGCCAACCCC CAGCTGGGAGCATGTCCTCTTCACTGCCTGCCACAACCAGCACCTGCAGCTGCAGCCCCCCCGCCGCAGG CTGCACTCGTGGGCGGACGACTGGAAGGCCATTCTGGACAGCAAGCGGCCCTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207737 protein sequence
Red=Cloning site Green=Tags(s) MIRLGGWCARRLCSAAVPAGRRGAAGGLGLAGGRALRVLVDMDGVLADFEGGFLRKFRARFPDQPFIALE DRRGFWVSEQYGRLRPGLSEKAISIWESKNFFFELEPLPGAVEAVKEMASLQNTDVFICTSPIKMFKYCP YEKYAWVEKYFGPDFLEQIVLTRDKTVVSADLLIDDRPDITGAEPTPSWEHVLFTACHNQHLQLQPPRRR LHSWADDWKAILDSKRPC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020201 |
| ORF Size | 684 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_020201.4 |
| RefSeq Size | 1633 bp |
| RefSeq ORF | 687 bp |
| Locus ID | 56953 |
| UniProt ID | Q9NPB1 |
| Protein Pathways | Metabolic pathways, Nicotinate and nicotinamide metabolism, Purine metabolism, Pyrimidine metabolism |
| MW | 25.9 kDa |
| Gene Summary | This gene encodes a 5' nucleotidase that localizes to the mitochondrial matrix. This enzyme dephosphorylates the 5'- and 2'(3')-phosphates of uracil and thymine deoxyribonucleotides. The gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Glycolytic flux control by drugging phosphoglycolate phosphatase
,null,
Nature Communications
,PubMed ID 36369173
[NT5M]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207737L3 | Lenti ORF clone of Human 5',3'-nucleotidase, mitochondrial (NT5M), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207737L4 | Lenti ORF clone of Human 5',3'-nucleotidase, mitochondrial (NT5M), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5,890.00 |
|
| RG207737 | NT5M (tGFP-tagged) - Human 5',3'-nucleotidase, mitochondrial (NT5M), nuclear gene encoding mitochondrial protein |
CNY 4,000.00 |
|
| SC122889 | NT5M (untagged)-Human 5',3'-nucleotidase, mitochondrial (NT5M), nuclear gene encoding mitochondrial protein |
CNY 2,400.00 |
