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TTC30B (NM_152517) Human Tagged ORF Clone
CAT＃: RC207665
TTC30B (Myc-DDK-tagged)-Human tetratricopeptide repeat domain 30B (TTC30B)
ORF Plasmid: tGFP
"NM_152517" in other vectors (5)
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CNY 4,952.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | fleer; IFT70; IFT70B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207665 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGGCCTGAGCGGCGCGCAGATCCCCGACGGGGAGTTCACCGCGGTCGTGTACCGCCTCATCCGCA ATGCACGCTACGCCGAGGCGGTGCAGCTGCTGGGCGGAGAACTGCAGCGGAGCCCTAGGAGCCGCGCCGG CCTGTCGCTGCTAGGCTACTGCTACTACCGCCTGCAGGAGTTCGCGCTGGCGGCCGAGTGCTATGAGCAG CTGGGCCAGCTGCACCCGGAACTGGAGCAGTACCGCCTGTACCAGGCCCAGGCCCTATACAAGGCCTGCC TTTATGCGGAGGCCACCCGGGTCGCCTTCCTTCTCCTGGATAACCCCGCCTACCACAGCCGGGTCCTCCA CCTGCAAGCTGCTATCAAGTACAGCGAGGGCGATCTGCCAGGGTCCAGGAGCCTGGTAGAGCAGCTGCCG AGTAGGGAAGGGGGAGAGGAAAGTGGGGGCGAGAATGAGACCGATGGCCAGATCAACCTGGGTTGTTTGC TCTACAAGGAGGGACAGTATGAAGCTGCATGCTCCAAGTTTTTTGCCGCCCTGCAGGCCTCGGGCTACCA GCCTGACCTTTCCTACAACCTGGCTTTGGCCTATTACAGCAGCCGACAGTATGCTTCAGCACTGAAGCAT ATCGCTGAGATTATTGAGCGTGGCATCCGCCAGCACCCTGAGCTAGGTGTGGGCATGACCACTGAGGGCA TTGATGTTCGCAGTGTTGGCAACACCTTAGTCCTCCATCAGACTGCTCTGGTGGAAGCCTTCAACCTTAA GGCAGCTATAGAATACCAACTGAGAAACTATGAGGCAGCTCAAGAAGCCCTCACTGACATGCCACCCAGG GCAGAGGAAGAGTTGGACCCTGTGACCCTACACAACCAGGCACTAATGAACATGGATGCCAGGCCTACAG AAGGGTTTGAAAAGCTACAGTTTTTGCTCCAACAGAATCCCTTTCCTCCAGAGACTTTTGGCAACCTGTT GCTGCTCTACTGTAAATATGAGTATTTTGACCTGGCAGCAGATGTCCTGGCAGAAAATGCCCATTTGATT TATAAGTTCCTCACACCCTATCTCTATGACTTCTTGGACGCTGTGATCACTTGCCAGACAGCTCCTGAAG AGGCTTTCATTAAGCTTGATGGGCTAGCAGGGATGCTGACTGAGGTCCTCCGGAAACTTACCATACAAGT ACAGGAAGCAAGACACAATAGAGATGATGAAGCTATCAAAAAGGCAGTGAATGAATATGATGAAACCATG GAGAAATACATTCCTGTGTTGATGGCTCAGGCAAAAATCTACTGGAATCTTGAAAATTATCCAATGGTGG AAAAGATCTTCCGCAAATCTGTGGAATTCTGTAACGACCATGATGTGTGGAAGTTGAATGTGGCTCATGT TCTGTTCATGCAGGAAAACAAATACAAAGAAGCCATTGGTTTCTATGAACCCATAGTCAAGAAGCATTAT GATAACATCCTGAATGTCAGTGCTATTGTACTGGCTAATCTCTGTGTTTCCTATATTATGACAAGTCAAA ATGAAGAAGCAGAGGAGTTGATGAGGAAGATTGAAAAGGAGGAAGAGCAGCTCTCTTATGATGACCCAGA TAAGAAAATGTACCATCTCTGCATTGTGAATTTGGTGATAGGAACTCTTTATTGTGCCAAAGGAAATTAT GACTTTGGTATTTCTCGAGTTATCAAAAGCTTGGAACCTTACAACAAAAAGCTGGGAACAGACACCTGGT ATTATGCCAAAAGATGCTTCCTGTCCTTGTTAGAAAACATGTCAAAACACACAATCATGCTTCGTGATAG TGTTATTCAAGAATGTGTCCAGTTTCTAGAACACTGTGAACTTCATGGCAGAAACATACCTGCTGTTATT GAACAACCCCTGGAAGAAGAAAGAATGCATGTTGGAAAGAATACAGTCACATATGAGTCTAGGCAGTTAA AAGCTTTGATTTATGAGATTATAGGATGGAATATA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207665 protein sequence
Red=Cloning site Green=Tags(s) MAGLSGAQIPDGEFTAVVYRLIRNARYAEAVQLLGGELQRSPRSRAGLSLLGYCYYRLQEFALAAECYEQ LGQLHPELEQYRLYQAQALYKACLYAEATRVAFLLLDNPAYHSRVLHLQAAIKYSEGDLPGSRSLVEQLP SREGGEESGGENETDGQINLGCLLYKEGQYEAACSKFFAALQASGYQPDLSYNLALAYYSSRQYASALKH IAEIIERGIRQHPELGVGMTTEGIDVRSVGNTLVLHQTALVEAFNLKAAIEYQLRNYEAAQEALTDMPPR AEEELDPVTLHNQALMNMDARPTEGFEKLQFLLQQNPFPPETFGNLLLLYCKYEYFDLAADVLAENAHLI YKFLTPYLYDFLDAVITCQTAPEEAFIKLDGLAGMLTEVLRKLTIQVQEARHNRDDEAIKKAVNEYDETM EKYIPVLMAQAKIYWNLENYPMVEKIFRKSVEFCNDHDVWKLNVAHVLFMQENKYKEAIGFYEPIVKKHY DNILNVSAIVLANLCVSYIMTSQNEEAEELMRKIEKEEEQLSYDDPDKKMYHLCIVNLVIGTLYCAKGNY DFGISRVIKSLEPYNKKLGTDTWYYAKRCFLSLLENMSKHTIMLRDSVIQECVQFLEHCELHGRNIPAVI EQPLEEERMHVGKNTVTYESRQLKALIYEIIGWNI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_152517 |
| ORF Size | 1995 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_152517.2, NP_689730.2 |
| RefSeq Size | 2652 bp |
| RefSeq ORF | 1998 bp |
| Locus ID | 150737 |
| UniProt ID | Q8N4P2 |
| MW | 76.1 kDa |
| Gene Summary | Required for polyglutamylation of axonemal tubulin. Plays a role in anterograde intraflagellar transport (IFT), the process by which cilia precursors are transported from the base of the cilium to the site of their incorporation at the tip.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207665L3 | Lenti ORF clone of Human tetratricopeptide repeat domain 30B (TTC30B), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC207665L4 | Lenti ORF clone of Human tetratricopeptide repeat domain 30B (TTC30B), mGFP tagged |
CNY 6,460.00 |
|
| RG207665 | TTC30B (tGFP-tagged) - Human tetratricopeptide repeat domain 30B (TTC30B) |
CNY 6,552.00 |
|
| SC306428 | TTC30B (untagged)-Human tetratricopeptide repeat domain 30B (TTC30B) |
CNY 7,220.00 |
|
| SC321757 | TTC30B (untagged)-Human tetratricopeptide repeat domain 30B (TTC30B) |
CNY 4,960.00 |
