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TLK1 (NM_012290) Human Tagged ORF Clone
CAT＃: RC207653
TLK1 (Myc-DDK-tagged)-Human tousled-like kinase 1 (TLK1), transcript variant 1
ORF Plasmid: tGFP
"NM_012290" in other vectors (6)
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CNY 5,608.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PKU-beta |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207653 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGTCCAAAGTAGCAGTGGAAGTTTGGAGGGGCCGCCATCTTGGTCCCAGCTCTCCACGTCTCCAA CCCCGGGCTCGGCGGCGGCGGCCAGGTCCCTGCTGAATCACACGCCGCCATCCGGGAGGCCCAGGGAAGG TGCAATGGATGAGCTTCATAGTCTGGATCCAAGAAGGCAAGAGTTATTGGAAGCTAGATTTACTGGAGTT GCAAGTGGGAGCACTGGAAGTACGGGCAGTTGCAGTGTTGGAGCTAAAGCCTCAACAAATAACGAAAGCT CTAATCACAGTTTTGGAAGCTTGGGATCTTTAAGTGACAAAGAATCAGAGACACCGGAGAAGAAACAATC GGAATCATCCAGGGGAAGAAAGAGAAAAGCAGAAAACCAGAATGAAAGTAGTCAGGGAAAAAGTATTGGG GGACGTGGCCACAAAATTAGCGACTATTTTGAATACCAGGGTGGAAATGGCTCAAGTCCAGTAAGAGGCA TACCTCCTGCAATCCGTTCTCCTCAAAATTCACATTCACATTCCACTCCTTCCTCATCTGTTCGACCGAA TAGCCCTTCTCCTACTGCATTAGCATTTGGGGACCACCCTATTGTACAACCAAAGCAATTATCCTTTAAA ATTATTCAGACTGATCTCACAATGCTGAAATTAGCAGCATTAGAAAGTAATAAAATCCAGGACCTGGAAA AGAAGGAGGGACGTATAGATGATTTGCTCAGGGCTAACTGTGATCTCAGACGGCAAATAGATGAACAACA AAAATTACTTGAAAAATACAAAGAACGATTAAATAAGTGCATATCAATGAGCAAGAAACTTCTTATTGAA AAGAGTACACAAGAAAAGCTGTCAAGCAGAGAGAAGAGTATGCAAGATCGATTACGCCTTGGGCACTTTA CAACAGTTAGACATGGCGCTTCATTTACTGAACAATGGACAGATGGTTTTGCATTTCAGAATCTTGTGAA GCAACAAGAATGGGTGAATCAGCAAAGGGAAGATATTGAAAGGCAAAGGAAACTTCTAGCCAAACGCAAA CCTCCCACAGCTAATAATTCTCAGGCACCCTCTACCAATTCTGAACCAAAACAAAGGAAAAACAAAGCAG TCAATGGAGCAGAGAATGATCCCTTTGTTAGACCAAATTTACCACAACTGTTGACTTTGGCAGAATATCA TGAACAGGAAGAAATTTTCAAACTTAGACTAGGACATCTCAAAAAGGAAGAGGCAGAAATCCAGGCAGAA CTTGAACGTTTGGAAAGAGTCAGAAATCTTCACATACGTGAGCTGAAAAGAATAAACAATGAAGATAATT CACAGTTCAAAGATCACCCAACATTAAATGAAAGATATTTATTACTTCATCTGCTTGGTAGAGGTGGCTT TAGTGAAGTGTATAAGGCTTTTGACCTTTATGAACAAAGATATGCTGCTGTGAAGATACATCAGCTTAAT AAAAGCTGGAGAGATGAGAAGAAAGAAAACTACCACAAACATGCCTGCAGAGAGTATAGAATACACAAAG AACTGGATCACCCCAGAATAGTTAAACTCTATGATTATTTCTCCTTGGATACAGATACGTTTTGTACAGT GTTAGAATACTGTGAAGGCAATGACTTGGATTTCTATCTGAAGCAACACAAGTTAATGTCAGAGAAAGAA GCTCGGTCTATTGTAATGCAGATTGTAAATGCACTAAGATATCTCAATGAGATCAAACCCCCTATTATAC ATTATGATCTTAAGCCAGGAAACATCCTACTGGTAGATGGAACAGCATGTGGTGAAATCAAAATCACTGA TTTTGGTCTGTCCAAGATTATGGATGATGATAGCTATGGTGTAGATGGAATGGATCTAACTTCCCAGGGG GCAGGCACTTACTGGTATTTACCTCCTGAGTGTTTTGTAGTTGGAAAAGAGCCACCAAAGATTTCCAACA AGGTTGATGTATGGTCGGTTGGAGTCATCTTCTTTCAGTGTCTTTATGGTAGAAAGCCATTTGGTCACAA TCAATCTCAACAAGACATTCTTCAAGAAAATACAATATTAAAAGCCACAGAAGTCCAGTTCCCTGTAAAA CCGGTTGTAAGCAGTGAAGCCAAGGCATTTATAAGACGCTGTTTGGCATATCGAAAAGAAGATCGATTTG ATGTGCACCAGCTGGCAAATGACCCATACCTTCTCCCACACATGAGAAGATCAAATTCTTCAGGAAACCT ACACATGGCTGGGCTGACAGCATCCCCTACACCCCCTTCTTCAAGCATAATTACTTAC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC207653 protein sequence
Red=Cloning site Green=Tags(s) MSVQSSSGSLEGPPSWSQLSTSPTPGSAAAARSLLNHTPPSGRPREGAMDELHSLDPRRQELLEARFTGV ASGSTGSTGSCSVGAKASTNNESSNHSFGSLGSLSDKESETPEKKQSESSRGRKRKAENQNESSQGKSIG GRGHKISDYFEYQGGNGSSPVRGIPPAIRSPQNSHSHSTPSSSVRPNSPSPTALAFGDHPIVQPKQLSFK IIQTDLTMLKLAALESNKIQDLEKKEGRIDDLLRANCDLRRQIDEQQKLLEKYKERLNKCISMSKKLLIE KSTQEKLSSREKSMQDRLRLGHFTTVRHGASFTEQWTDGFAFQNLVKQQEWVNQQREDIERQRKLLAKRK PPTANNSQAPSTNSEPKQRKNKAVNGAENDPFVRPNLPQLLTLAEYHEQEEIFKLRLGHLKKEEAEIQAE LERLERVRNLHIRELKRINNEDNSQFKDHPTLNERYLLLHLLGRGGFSEVYKAFDLYEQRYAAVKIHQLN KSWRDEKKENYHKHACREYRIHKELDHPRIVKLYDYFSLDTDTFCTVLEYCEGNDLDFYLKQHKLMSEKE ARSIVMQIVNALRYLNEIKPPIIHYDLKPGNILLVDGTACGEIKITDFGLSKIMDDDSYGVDGMDLTSQG AGTYWYLPPECFVVGKEPPKISNKVDVWSVGVIFFQCLYGRKPFGHNQSQQDILQENTILKATEVQFPVK PVVSSEAKAFIRRCLAYRKEDRFDVHQLANDPYLLPHMRRSNSSGNLHMAGLTASPTPPSSSIITY SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012290 |
| ORF Size | 2298 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_012290.3, NP_036422.3 |
| RefSeq Size | 5751 bp |
| RefSeq ORF | 2301 bp |
| Locus ID | 9874 |
| UniProt ID | Q9UKI8 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 86.7 kDa |
| Gene Summary | The protein encoded by this gene is a serine/threonine kinase that may be involved in the regulation of chromatin assembly. The encoded protein is only active when it is phosphorylated, and this phosphorylation is cell cycle-dependent, with the maximal activity of this protein coming during S phase. The catalytic activity of this protein is diminished by DNA damage and by blockage of DNA replication. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207653L1 | Lenti ORF clone of Human tousled-like kinase 1 (TLK1), transcript variant 1, Myc-DDK-tagged |
CNY 8,008.00 |
|
| RC207653L2 | Lenti ORF clone of Human tousled-like kinase 1 (TLK1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC207653L3 | Lenti ORF clone of Human tousled-like kinase 1 (TLK1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207653L4 | Lenti ORF clone of Human tousled-like kinase 1 (TLK1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG207653 | TLK1 (tGFP-tagged) - Human tousled-like kinase 1 (TLK1), transcript variant 1 |
CNY 7,208.00 |
|
| SC108453 | TLK1 (untagged)-Human tousled-like kinase 1 (TLK1), transcript variant 1 |
CNY 5,616.00 |
