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DNA Polymerase iota (POLI) (NM_007195) Human Tagged ORF Clone
CAT＃: RC207550
POLI (Myc-DDK-tagged)-Human polymerase (DNA directed) iota (POLI)
ORF Plasmid: tGFP
"NM_007195" in other vectors (7)
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CNY 5,240.00
CNY 5,800.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | eta2; RAD3OB; RAD30B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207550 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACTGGCGGACGTGGGGGCGGCAGCCAGCTCGCAGGGAGTTCATGATCAAGTGTTGCCCACACCAA ATGCTTCATCCAGAGTCATAGTACATGTGGATCTGGATTGCTTTTATGCACAAGTAGAAATGATCTCAAA TCCAGAGCTAAAAGACAAACCTTTAGGGGTTCAACAGAAATATTTGGTGGTTACCTGCAACTATGAAGCT AGGAAACTTGGAGTTAAGAAACTTATGAATGTCAGAGATGCAAAAGAAAAGTGTCCACAGTTGGTATTAG TTAATGGAGAAGACCTGACCCGCTACAGAGAAATGTCTTATAAGGTTACAGAATTACTGGAAGAATTTAG TCCAGTTGTTGAGAGACTTGGATTTGATGAAAATTTTGTGGATCTAACAGAAATGGTTGAGAAGAGACTA CAGCAGCTGCAAAGTGATGAACTTTCTGCGGTGACTGTGTCGGGTCATGTATACAATAATCAGTCTATAA ACCTGCTTGACGTCTTGCACATCAGACTACTTGTTGGATCTCAGATTGCAGCAGAGATGCGGGAAGCCAT GTATAATCAGTTGGGGCTCACTGGCTGTGCTGGAGTGGCTTCTAATAAACTGTTGGCAAAATTAGTTTCT GGTGTCTTTAAACCAAATCAACAAACAGTCTTATTACCTGAAAGTTGTCAACATCTTATTCATAGTTTGA ATCACATAAAGGAAATACCTGGTATTGGCTATAAAACTGCCAAATGTCTTGAAGCACTGGGTATCAATAG TGTGCGTGATCTCCAAACCTTTTCACCCAAAATTTTAGAAAAAGAATTAGGAATTTCAGTTGCTCAGCGT ATCCAAAAGCTCAGTTTTGGAGAGGATAACTCCCCTGTGATACTCTCAGGACCACCTCAGTCCTTTAGTG AAGAAGATTCATTTAAAAAATGTTCATCTGAAGTTGAAGCTAAAAATAAGATTGAAGAACTACTTGCTAG TCTTTTAAACAGAGTATGCCAAGATGGAAGGAAGCCTCATACAGTGAGATTAATAATCCGTCGGTATTCC TCTGAGAAGCACTATGGTCGTGAGAGTCGTCAGTGCCCTATTCCTTCACATGTAATTCAGAAATTAGGGA CAGGAAATTATGATGTGATGACCCCAATGGTTGATATACTTATGAAACTTTTTCGAAATATGGTGAATGT GAAGATGCCATTTCACCTTACCCTTCTAAGTGTGTGCTTCTGCAACCTTAAAGCACTAAATACTGCTAAG AAAGGGCTTATTGATTATTATTTAATGCCATCATTATCAACTACTTCACGCTCTGGCAAGCACAGTTTTA AAATGAAAGACACTCATATGGAAGATTTTCCCAAAGACAAAGAAACAAACCGGGATTTCCTACCAAGTGG AAGAATTGAAAGTACAAGAACTAGGGAGTCTCCACTAGATACCACAAATTTTTCTAAAGAAAAAGACATT AATGAATTCCCACTCTGTTCACTTCCTGAAGGTGTTGACCAAGAAGTCTTCAAGCAGCTTCCAGTAGATA TTCAAGAAGAAATCCTTTCTGGAAAATCTAGGGAAAAATTTCAAGGGAAAGGAAGTGTGAGTTGTCCATT ACATGCCTCTAGAGGAGTATTATCTTTCTTTTCTAAAAAACAAATGCAAGATATTCCCATAAATCCTAGA GATCATTTATCCAGTAGCAAACAGGTATCCTCTGTATCTCCTTGTGAACCGGGAACATCAGGCTTTAATA GCAGTAGTTCTTCTTACATGTCTAGCCAAAAGGATTATTCATATTATTTAGATAATAGATTAAAAGATGA ACGAATAAGTCAAGGACCTAAAGAACCTCAAGGATTCCACTTTACAAATTCAAACCCTGCTGTGTCTGCT TTTCATTCATTTCCAAACTTGCAGAGTGAGCAACTTTTCTCCAGAAACCACACTACAGATAGCCATAAGC AAACAGTAGCAACAGACTCTCATGAAGGACTTACAGAAAATAGAGAGCCAGATTCTGTTGATGAGAAAAT TACTTTCCCTTCTGACATTGATCCTCAAGTTTTCTATGAACTACCAGAAGCAGTACAAAAGGAACTGCTG GCAGAGTGGAAGAGAGCAGGATCAGATTTCCACATTGGACATAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207550 protein sequence
Red=Cloning site Green=Tags(s) MELADVGAAASSQGVHDQVLPTPNASSRVIVHVDLDCFYAQVEMISNPELKDKPLGVQQKYLVVTCNYEA RKLGVKKLMNVRDAKEKCPQLVLVNGEDLTRYREMSYKVTELLEEFSPVVERLGFDENFVDLTEMVEKRL QQLQSDELSAVTVSGHVYNNQSINLLDVLHIRLLVGSQIAAEMREAMYNQLGLTGCAGVASNKLLAKLVS GVFKPNQQTVLLPESCQHLIHSLNHIKEIPGIGYKTAKCLEALGINSVRDLQTFSPKILEKELGISVAQR IQKLSFGEDNSPVILSGPPQSFSEEDSFKKCSSEVEAKNKIEELLASLLNRVCQDGRKPHTVRLIIRRYS SEKHYGRESRQCPIPSHVIQKLGTGNYDVMTPMVDILMKLFRNMVNVKMPFHLTLLSVCFCNLKALNTAK KGLIDYYLMPSLSTTSRSGKHSFKMKDTHMEDFPKDKETNRDFLPSGRIESTRTRESPLDTTNFSKEKDI NEFPLCSLPEGVDQEVFKQLPVDIQEEILSGKSREKFQGKGSVSCPLHASRGVLSFFSKKQMQDIPINPR DHLSSSKQVSSVSPCEPGTSGFNSSSSSYMSSQKDYSYYLDNRLKDERISQGPKEPQGFHFTNSNPAVSA FHSFPNLQSEQLFSRNHTTDSHKQTVATDSHEGLTENREPDSVDEKITFPSDIDPQVFYELPEAVQKELL AEWKRAGSDFHIGHK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007195 |
| ORF Size | 2145 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007195.1, NP_009126.1 |
| RefSeq Size | 6074 bp |
| RefSeq ORF | 2223 bp |
| Locus ID | 11201 |
| UniProt ID | Q9UNA4 |
| Domains | IMS |
| Protein Families | Druggable Genome |
| MW | 80.3 kDa |
| Gene Summary | The protein encoded by this gene is an error-prone DNA polymerase involved in DNA repair. The encoded protein promotes DNA synthesis across lesions in the template DNA, which other polymerases cannot do. The encoded polymerase inserts deoxynucleotides across lesions and then relies on DNA polymerase zeta to extend the nascent DNA strand to bypass the lesion. [provided by RefSeq, May 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207550L1 | Lenti ORF clone of Human polymerase (DNA directed) iota (POLI), Myc-DDK-tagged |
CNY 7,640.00 |
|
| RC207550L2 | Lenti ORF clone of Human polymerase (DNA directed) iota (POLI), mGFP tagged |
CNY 7,700.00 |
|
| RC207550L3 | Lenti ORF clone of Human polymerase (DNA directed) iota (POLI), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC207550L4 | Lenti ORF clone of Human polymerase (DNA directed) iota (POLI), mGFP tagged |
CNY 7,700.00 |
|
| RG207550 | POLI (tGFP-tagged) - Human polymerase (DNA directed) iota (POLI) |
CNY 6,840.00 |
|
| SC318221 | POLI (untagged)-Human polymerase (DNA directed) iota (POLI) |
CNY 14,160.00 |
|
| SC318222 | POLI (untagged)-Human polymerase (DNA directed) iota (POLI) |
CNY 14,160.00 |
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