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E74 like factor 1 (ELF1) (NM_172373) Human Tagged ORF Clone
CAT＃: RC207140
- TrueORF®
ELF1 (Myc-DDK-tagged)-Human E74-like factor 1 (ets domain transcription factor) (ELF1), transcript variant 1
ORF Plasmid: tGFP
"NM_172373" in other vectors (5)
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CNY 4,560.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EFTUD1; RIA1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207140 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCTGTTGTCCAACAGAACGACCTAGTATTTGAATTTGCTAGTAACGTCATGGAGGATGAACGAC AGCTTGGTGATCCAGCTATTTTTCCTGCCGTAATTGTGGAACATGTTCCTGGTGCTGATATTCTCAATAG TTATGCCGGTCTAGCCTGTGTGGAAGAGCCCAATGACATGATTACTGAGAGTTCACTGGATGTTGCTGAA GAAGAAATCATAGACGATGATGATGATGACATCACCCTTACAGTTGAAGCTTCTTGTCATGACGGGGATG AAACAATTGAAACTATTGAGGCTGCTGAGGCACTCCTCAATATGGATTCCCCTGGCCCTATGCTGGATGA AAAACGAATAAATAATAATATATTTAGTTCACCTGAAGATGACATGGTTGTTGCCCCAGTCACCCATGTG TCCGTCACATTAGATGGGATTCCTGAAGTGATGGAAACACAGCAGGTGCAAGAAAAATATGCAGACTCAC CGGGAGCCTCATCACCAGAACAGCCTAAGAGGAAAAAAGGAAGAAAAACTAAACCACCACGACCAGATTC CCCAGCCACTACGCCAAATATATCTGTGAAGAAGAAAAACAAAGATGGAAAGGGAAACACAATTTATCTT TGGGAGTTTTTACTGGCACTGCTCCAGGACAAGGCTACTTGTCCTAAATACATCAAGTGGACCCAGCGAG AGAAAGGCATTTTTAAATTGGTGGATTCTAAAGCAGTGTCCAGGTTGTGGGGGAAGCACAAAAACAAACC TGATATGAATTATGAGACCATGGGAAGAGCACTCAGGTACTATTACCAAAGGGGTATTCTGGCAAAAGTG GAAGGTCAGCGCTTGGTGTATCAGTTTAAAGAAATGCCAAAAGATCTTATATATATAAATGATGAGGATC CAAGTTCCAGCATAGAGTCTTCAGATCCATCACTATCTTCATCAGCCACTTCAAATAGGAATCAAACCAG CCGGTCGAGAGTATCTTCAAGTCCAGGGGTAAAAGGAGGAGCCACTACAGTTCTAAAACCAGGGAATTCT AAAGCTGCAAAACCCAAAGATCCTGTGGAAGTTGCACAACCATCAGAAGTTTTGAGGACAGTGCAGCCCA CGCAGTCTCCATATCCTACCCAGCTCTTCCGGACTGTTCATGTAGTACAGCCAGTACAGGCTGTCCCAGA GGGAGAAGCAGCTAGAACCAGTACCATGCAGGATGAAACATTAAATTCTTCCGTTCAGAGTATTAGGACT ATACAGGCTCCAACCCAAGTTCCAGTGGTTGTGTCTCCTAGGAATCAGCAGTTGCATACAGTAACACTCC AAACAGTGCCACTCACAACAGTTATAGCCAGCACAGATCCATCAGCAGGTACTGGATCTCAGAAGTTTAT TTTACAAGCCATTCCATCATCACAGCCCATGACAGTACTGAAAGAAAATGTCATGCTGCAGTCACAAAAG GCGGGCTCTCCTCCTTCAATTGTCTTGGGCCCTGCCCAGGTTCAGCAGGTCCTTACTAGCAATGTTCAGA CCATTTGCAATGGAACCGTCAGTGTGGCTTCCTCTCCATCCTTCAGTGCTACTGCACCTGTGGTGACCTT TTCTCCTCGCAGTTCACAGCTGGTTGCTCACCCACCTGGCACTGTAATCACTTCAGTTATCAAAACTCAA GAAACAAAAACTCTTACACAGGAAGTAGAGAAAAAGGAATCTGAAGATCATTTGAAAGAGAACACTGAGA AAACGGAGCAGCAGCCACAGCCTTATGTGATGGTAGTGTCCAGTTCCAATGGATTTACTTCTCAGGTAGC TATGAAACAAAACGAACTGCTGGAACCCAACTCTTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207140 protein sequence
Red=Cloning site Green=Tags(s) MAAVVQQNDLVFEFASNVMEDERQLGDPAIFPAVIVEHVPGADILNSYAGLACVEEPNDMITESSLDVAE EEIIDDDDDDITLTVEASCHDGDETIETIEAAEALLNMDSPGPMLDEKRINNNIFSSPEDDMVVAPVTHV SVTLDGIPEVMETQQVQEKYADSPGASSPEQPKRKKGRKTKPPRPDSPATTPNISVKKKNKDGKGNTIYL WEFLLALLQDKATCPKYIKWTQREKGIFKLVDSKAVSRLWGKHKNKPDMNYETMGRALRYYYQRGILAKV EGQRLVYQFKEMPKDLIYINDEDPSSSIESSDPSLSSSATSNRNQTSRSRVSSSPGVKGGATTVLKPGNS KAAKPKDPVEVAQPSEVLRTVQPTQSPYPTQLFRTVHVVQPVQAVPEGEAARTSTMQDETLNSSVQSIRT IQAPTQVPVVVSPRNQQLHTVTLQTVPLTTVIASTDPSAGTGSQKFILQAIPSSQPMTVLKENVMLQSQK AGSPPSIVLGPAQVQQVLTSNVQTICNGTVSVASSPSFSATAPVVTFSPRSSQLVAHPPGTVITSVIKTQ ETKTLTQEVEKKESEDHLKENTEKTEQQPQPYVMVVSSSNGFTSQVAMKQNELLEPNSF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_172373 |
| ORF Size | 1857 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_172373.2, NP_758961.1 |
| RefSeq Size | 3760 bp |
| RefSeq ORF | 1860 bp |
| Locus ID | 1997 |
| UniProt ID | P32519 |
| Protein Families | Transcription Factors |
| MW | 67.5 kDa |
| Gene Summary | This gene encodes an E26 transformation-specific related transcription factor. The encoded protein is primarily expressed in lymphoid cells and acts as both an enhancer and a repressor to regulate transcription of various genes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207140L3 | Lenti ORF clone of Human E74-like factor 1 (ets domain transcription factor) (ELF1), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC207140L4 | Lenti ORF clone of Human E74-like factor 1 (ets domain transcription factor) (ELF1), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RG207140 | ELF1 (tGFP-tagged) - Human E74-like factor 1 (ets domain transcription factor) (ELF1), transcript variant 1 |
CNY 5,040.00 |
|
| SC128084 | ELF1 (untagged)-Human E74-like factor 1 (ets domain transcription factor) (ELF1), transcript variant 1 |
CNY 2,950.00 |
|
| SC324684 | ELF1 (untagged)-Human E74-like factor 1 (ets domain transcription factor) (ELF1), transcript variant 1 |
CNY 2,950.00 |
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