Epsin 1 (EPN1) (NM_013333) Human Tagged ORF Clone
CAT#: RC207099
EPN1 (Myc-DDK-tagged)-Human epsin 1 (EPN1), transcript variant 3
ORF Plasmid: tGFP
"NM_013333" in other vectors (6)
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CNY 4,096.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC207099 representing NM_013333
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGACCTCGTCCTTGAGGCGCCAGATGAAGAACATCGTCCACAACTACTCAGAGGCGGAGATCAAGG TTCGAGAGGCCACGAGCAATGACCCCTGGGGCCCATCCAGCTCCCTCATGTCAGAGATTGCCGACCTCAC CTACAACGTTGTCGCCTTCTCGGAGATCATGAGCATGATCTGGAAGCGGCTCAATGACCATGGCAAGAAC TGGCGTCACGTTTACAAGGCCATGACGCTGATGGAGTACCTCATCAAGACCGGCTCGGAGCGCGTGTCGC AGCAGTGCAAGGAGAACATGTACGCCGTGCAGACGCTGAAGGACTTCCAGTACGTGGACCGCGACGGCAA GGACCAGGGCGTGAACGTGCGTGAGAAAGCTAAGCAGCTGGTGGCCCTGCTGCGCGACGAGGACCGGCTG CGGGAAGAGCGGGCGCACGCGCTCAAGACCAAGGAAAAGCTGGCACAGACCGCCACGGCCTCATCAGCAG CTGTGGGCTCAGGCCCCCCTCCCGAGGCGGAGCAGGCGTGGCCGCAGAGCAGCGGGGAGGAGGAGCTGCA GCTCCAGCTGGCCCTGGCCATGAGCAAGGAGGAGGCCGACCAGGAGGAGCGGATCCGTCGCGGGGATGAC CTGCGGCTGCAGATGGCAATCGAGGAGAGCAAGAGGGAGACTGGGGGCAAGGAGGAGTCGTCCCTCATGG ACCTTGCTGACGTCTTCACGGCCCCAGCTCCTGCCCCGACCACAGACCCCTGGGGGGGCCCAGCACCCAT GGCTGCTGCCGTCCCCACGGCTGCCCCCACCTCGGACCCCTGGGGCGGCCCCCCTGTCCCTCCAGCTGCT GATCCCTGGGGAGGTCCAGCCCCCACGCCGGCCTCTGGGGACCCCTGGAGGCCTGCTGCCCCTGCAGGAC CCTCAGTTGACCCTTGGGGTGGGACCCCAGCCCCTGCAGCTGGGGAGGGGCCCACGCCTGATCCATGGGG AAGTTCCGATGGTGGGGTCCCGGTCAGTGGGCCCTCAGCCTCCGATCCCTGGACACCGGCCCCGGCCTTC TCAGATCCCTGGGGAGGGTCACCTGCCAAGCCCAGCACCAATGGCACAACAGCCGGGGGATTCGACACGG AGCCCGACGAGTTCTCTGACTTTGACCGACTCCGCACGGCACTGCCGACCTCCGGGAGCAGCGCAGGAGA GCTGGAGCTGCTGGCAGGAGAGGTGCCGGCCCGAAGCCCTGGGGCGTTTGACATGAGTGGGGTCAGGGGA TCTCTGGCTGAGGCTGTGGGCAGCCCCCCACCTGCAGCCACACCAACTCCCACGCCCCCCACCCGGAAGA CGCCGGAGTCATTCCTGGGGCCCAATGCAGCCCTCGTCGACCTGGACTCGCTGGTGAGCCGGCCGGGCCC CACGCCGCCTGGAGCCAAGGCCTCCAACCCCTTCCTGCCAGGCGGAGGCCCAGCCACTGGCCCTTCCGTC ACCAACCCCTTCCAGCCCGCGCCTCCCGCGACGCTCACCCTGAACCAGCTCCGTCTCAGTCCTGTGCCTC CCGTCCCTGGAGCGCCACCCACGTACATCTCTCCCCTTGGCGGGGGCCCTGGCCTGCCCCCCATGATGCC CCCGGGCCCCCCGGCCCCCAACACTAATCCCTTCCTCCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207099 representing NM_013333
Red=Cloning site Green=Tags(s) MSTSSLRRQMKNIVHNYSEAEIKVREATSNDPWGPSSSLMSEIADLTYNVVAFSEIMSMIWKRLNDHGKN WRHVYKAMTLMEYLIKTGSERVSQQCKENMYAVQTLKDFQYVDRDGKDQGVNVREKAKQLVALLRDEDRL REERAHALKTKEKLAQTATASSAAVGSGPPPEAEQAWPQSSGEEELQLQLALAMSKEEADQEERIRRGDD LRLQMAIEESKRETGGKEESSLMDLADVFTAPAPAPTTDPWGGPAPMAAAVPTAAPTSDPWGGPPVPPAA DPWGGPAPTPASGDPWRPAAPAGPSVDPWGGTPAPAAGEGPTPDPWGSSDGGVPVSGPSASDPWTPAPAF SDPWGGSPAKPSTNGTTAGGFDTEPDEFSDFDRLRTALPTSGSSAGELELLAGEVPARSPGAFDMSGVRG SLAEAVGSPPPAATPTPTPPTRKTPESFLGPNAALVDLDSLVSRPGPTPPGAKASNPFLPGGGPATGPSV TNPFQPAPPATLTLNQLRLSPVPPVPGAPPTYISPLGGGPGLPPMMPPGPPAPNTNPFLL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_013333 |
ORF Size | 1650 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_013333.3 |
RefSeq Size | 2430 bp |
RefSeq ORF | 1653 bp |
Locus ID | 29924 |
UniProt ID | Q9Y6I3 |
Domains | ENTH, UIM |
Protein Pathways | Endocytosis |
MW | 57.3 kDa |
Gene Summary | This gene encodes a member of the epsin protein family. The encoded protein binds clathrin and is involved in the endocytosis of clathrin-coated vesicles. Loss of function of this gene is associated with reduced tumor growth and progression in certain cancer types. [provided by RefSeq, Mar 2016] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
---|
Spatiotemporal control of endocytosis by phosphatidylinositol-3,4-bisphosphate
,York Posor, Marielle Eichhorn-Gruenig, Dmytro Puchkov, Johannes Schöneberg, Alexander Ullrich, + et al.,
Nature 499, 233-237 doi:10.1038/nature12360
,PubMed ID 23823722
[Epsin 1]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC207099L1 | Lenti ORF clone of Human epsin 1 (EPN1), transcript variant 3, Myc-DDK-tagged |
CNY 6,496.00 |
|
RC207099L2 | Lenti ORF clone of Human epsin 1 (EPN1), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
RC207099L3 | Lenti ORF clone of Human epsin 1 (EPN1), transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC207099L4 | Lenti ORF clone of Human epsin 1 (EPN1), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
RG207099 | EPN1 (tGFP-tagged) - Human epsin 1 (EPN1), transcript variant 3 |
CNY 5,696.00 |
|
SC321861 | EPN1 (untagged)-Human epsin 1 (EPN1), transcript variant 3 |
CNY 4,104.00 |
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