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CD99L2 (NM_031462) Human Tagged ORF Clone
CAT＃: RC207079
CD99L2 (Myc-DDK-tagged)-Human CD99 molecule-like 2 (CD99L2), transcript variant 1
ORF Plasmid: tGFP
"NM_031462" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CD99B; MIC2L1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207079 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGCCTGGCGCTCGGCGTTCCTTGTCTGCCTCGCTTTCTCCTTGGCCACCCTGGTCCAGCGAGGAT CTGGGGACTTTGATGATTTTAACCTGGAGGATGCAGTGAAAGAAACTTCCTCAGTAAAGCAGCCATGGGA CCACACCACCACCACCACAACCAATAGGCCAGGAACCACCAGAGCTCCGGCAAAACCTCCAGGTAGTGGA TTGGACTTGGCTGATGCTTTGGATGATCAAGATGATGGCCGCAGGAAACCGGGTATAGGAGGAAGAGAGA GATGGAACCATGTAACCACCACGACCAAGAGGCCAGTAACCACCAGAGCTCCAGCAAATACTTTAGGAAA TGATTTTGACTTGGCTGATGCCCTGGATGATCGAAATGATCGAGATGATGGCCGCAGGAAACCAATTGCT GGAGGAGGAGGTTTTTCAGACAAGGATCTTGAAGACATAGTAGGGGGTGGAGAATACAAACCTGACAAGG GTAAAGGTGATGGCCGGTACGGCAGCAATGACGACCCTGGATCTGGCATGGTGGCAGAGCCTGGCACCAT TGCCGGGGTGGCCAGCGCCCTGGCCATGGCCCTCATCGGTGCCGTCTCCAGCTACATCTCCTACCAGCAG AAGAAGTTCTGCTTCAGCATTCAGCAGGGTCTCAACGCAGACTACGTGAAGGGAGAGAACCTGGAAGCCG TGGTATGTGAGGAACCCCAAGTGAAATACTCCACGTTGCACACGCAGTCTGCAGAGCCGCCGCCGCCGCC CGAACCAGCCCGGATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207079 protein sequence
Red=Cloning site Green=Tags(s) MVAWRSAFLVCLAFSLATLVQRGSGDFDDFNLEDAVKETSSVKQPWDHTTTTTTNRPGTTRAPAKPPGSG LDLADALDDQDDGRRKPGIGGRERWNHVTTTTKRPVTTRAPANTLGNDFDLADALDDRNDRDDGRRKPIA GGGGFSDKDLEDIVGGGEYKPDKGKGDGRYGSNDDPGSGMVAEPGTIAGVASALAMALIGAVSSYISYQQ KKFCFSIQQGLNADYVKGENLEAVVCEEPQVKYSTLHTQSAEPPPPPEPARI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_031462 |
| ORF Size | 786 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_031462.4 |
| RefSeq Size | 3736 bp |
| RefSeq ORF | 789 bp |
| Locus ID | 83692 |
| UniProt ID | Q8TCZ2 |
| Protein Families | Transmembrane |
| MW | 28 kDa |
| Gene Summary | This gene encodes a cell-surface protein that is similar to CD99. A similar protein in mouse functions as an adhesion molecule during leukocyte extravasation. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Ectodomain shedding of CD99 within highly conserved regions is mediated by the metalloprotease meprin ß and promotes transendothelial cell migration
,Bedau, T;Peters, F;Prox, J;Arnold, P;Schmidt, F;Finkernagel, M;Köllmann, S;Wichert, R;Otte, A;Ohler, A;Stirnberg, M;Lucius, R;Koudelka, T;Tholey, A;Biasin, V;Pietrzik, CU;Kwapiszewska, G;Becker-Pauly, C;,
FASEB J
,PubMed ID 28003343
[CD99L2]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207079L3 | Lenti ORF clone of Human CD99 molecule-like 2 (CD99L2), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207079L4 | Lenti ORF clone of Human CD99 molecule-like 2 (CD99L2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG207079 | CD99L2 (tGFP-tagged) - Human CD99 molecule-like 2 (CD99L2), transcript variant 1 |
CNY 4,000.00 |
|
| SC111457 | CD99L2 (untagged)-Human CD99 molecule-like 2 (CD99L2), transcript variant 1 |
CNY 2,400.00 |
