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SIAH1 (NM_003031) Human Tagged ORF Clone
CAT＃: RC206576
SIAH1 (Myc-DDK-tagged)-Human seven in absentia homolog 1 (Drosophila) (SIAH1), transcript variant 1
ORF Plasmid: tGFP
"NM_003031" in other vectors (7)
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CNY 3,600.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BURHAS; SIAH1A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206576 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGGAAAGGCTACTCCACCTTCTCTGTACTCCTGGAGGGGAGTCTTGTTCACATGTTTACCAGCGG CCAGGACAAGGAAGAGAAAAGAAATGAGCCGTCAGACTGCTACAGCATTACCTACCGGTACCTCGAAGTG TCCACCATCCCAGAGGGTGCCTGCCCTGACTGGCACAACTGCATCCAACAATGACTTGGCGAGTCTTTTT GAGTGTCCAGTCTGCTTTGACTATGTGTTACCGCCCATTCTTCAATGTCAGAGTGGCCATCTTGTTTGTA GCAACTGTCGCCCAAAGCTCACATGTTGTCCAACTTGCCGGGGCCCTTTGGGATCCATTCGCAACTTGGC TATGGAGAAAGTGGCTAATTCAGTACTTTTCCCCTGTAAATATGCGTCTTCTGGATGTGAAATAACTCTG CCACACACAGAAAAAGCAGACCATGAAGAGCTCTGTGAGTTTAGGCCTTATTCCTGTCCGTGCCCTGGTG CTTCCTGTAAATGGCAAGGCTCTCTGGATGCTGTAATGCCCCATCTGATGCATCAGCATAAGTCCATTAC AACCCTACAGGGAGAGGATATAGTTTTTCTTGCTACAGACATTAATCTTCCTGGTGCTGTTGACTGGGTG ATGATGCAGTCCTGTTTTGGCTTTCACTTCATGTTAGTCTTAGAGAAACAGGAAAAATACGATGGTCACC AGCAGTTCTTCGCAATCGTACAGCTGATAGGAACACGCAAGCAAGCTGAAAATTTTGCTTACCGACTTGA GCTAAATGGTCATAGGCGACGATTGACTTGGGAAGCGACTCCTCGATCTATTCATGAAGGAATTGCAACA GCCATTATGAATAGCGACTGTCTAGTCTTTGACACCAGCATTGCACAGCTTTTTGCAGAAAATGGCAATT TAGGCATCAATGTAACTATTTCCATGTGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206576 protein sequence
Red=Cloning site Green=Tags(s) MTGKATPPSLYSWRGVLFTCLPAARTRKRKEMSRQTATALPTGTSKCPPSQRVPALTGTTASNNDLASLF ECPVCFDYVLPPILQCQSGHLVCSNCRPKLTCCPTCRGPLGSIRNLAMEKVANSVLFPCKYASSGCEITL PHTEKADHEELCEFRPYSCPCPGASCKWQGSLDAVMPHLMHQHKSITTLQGEDIVFLATDINLPGAVDWV MMQSCFGFHFMLVLEKQEKYDGHQQFFAIVQLIGTRKQAENFAYRLELNGHRRRLTWEATPRSIHEGIAT AIMNSDCLVFDTSIAQLFAENGNLGINVTISMC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003031 |
| ORF Size | 939 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq Size | 2003 bp |
| RefSeq ORF | 849 bp |
| Locus ID | 6477 |
| UniProt ID | Q8IUQ4 |
| Domains | Sina |
| Protein Families | Druggable Genome |
| Protein Pathways | p53 signaling pathway, Ubiquitin mediated proteolysis, Wnt signaling pathway |
| MW | 34.6 kDa |
| Gene Summary | This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Clinical and molecular spectrum of CHOPS syndrome
,Raible, SE;Mehta, D;Bettale, C;Fiordaliso, S;Kaur, M;Medne, L;Rio, M;Haan, E;White, SM;Cusmano-Ozog, K;Nishi, E;Guo, Y;Wu, H;Shi, X;Zhao, Q;Zhang, X;Lei, Q;Lu, A;He, X;Okamoto, N;Miyake, N;Piccione, J;Allen, J;Matsumoto, N;Pipan, M;Krantz, ID;Izumi, K;,
Am. J. Med. Genet. A
,PubMed ID 31058441
[SIAH1]
|
|
Germline gain-of-function mutations in AFF4 cause a developmental syndrome functionally linking the super elongation complex and cohesin
,Izumi, K;Nakato, R;Zhang, Z;Edmondson, AC;Noon, S;Dulik, MC;Rajagopalan, R;Venditti, CP;Gripp, K;Samanich, J;Zackai, EH;Deardorff, MA;Clark, D;Allen, JL;Dorsett, D;Misulovin, Z;Komata, M;Bando, M;Kaur, M;Katou, Y;Shirahige, K;Krantz, ID;,
Nat. Genet.
,PubMed ID 25730767
[SIAH1]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206576L1 | Lenti ORF clone of Human seven in absentia homolog 1 (Drosophila) (SIAH1), transcript variant 1, Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC206576L2 | Lenti ORF clone of Human seven in absentia homolog 1 (Drosophila) (SIAH1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC206576L3 | Lenti ORF clone of Human seven in absentia homolog 1 (Drosophila) (SIAH1), transcript variant 1, Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC206576L4 | Lenti ORF clone of Human seven in absentia homolog 1 (Drosophila) (SIAH1), transcript variant 1, mGFP tagged |
CNY 6,000.00 |
|
| RG206576 | SIAH1 (tGFP-tagged) - Human seven in absentia homolog 1 (Drosophila) (SIAH1), transcript variant 1 |
CNY 5,200.00 |
|
| SC118259 | SIAH1 (untagged)-Human seven in absentia homolog 1 (Drosophila) (SIAH1), transcript variant 1 |
CNY 2,400.00 |
|
| SC323995 | SIAH1 (untagged)-Human seven in absentia homolog 1 (Drosophila) (SIAH1), transcript variant 1 |
CNY 2,400.00 |
