Myocilin (MYOC) (NM_000261) Human Tagged ORF Clone
CAT#: RC206556
MYOC (Myc-DDK-tagged)-Human myocilin, trabecular meshwork inducible glucocorticoid response (MYOC)
ORF Plasmid: tGFP
"NM_000261" in other vectors (6)
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CNY 5,632.00
Cited in 5 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | GLC1A; GPOA; JOAG; JOAG1; TIGR |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC206556 representing NM_000261
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGTTCTTCTGTGCACGTTGCTGCAGCTTTGGGCCTGAGATGCCAGCTGTCCAGCTGCTGCTTCTGG CCTGCCTGGTGTGGGATGTGGGGGCCAGGACAGCTCAGCTCAGGAAGGCCAATGACCAGAGTGGCCGATG CCAGTATACCTTCAGTGTGGCCAGTCCCAATGAATCCAGCTGCCCAGAGCAGAGCCAGGCCATGTCAGTC ATCCATAACTTACAGAGAGACAGCAGCACCCAACGCTTAGACCTGGAGGCCACCAAAGCTCGACTCAGCT CCCTGGAGAGCCTCCTCCACCAATTGACCTTGGACCAGGCTGCCAGGCCCCAGGAGACCCAGGAGGGGCT GCAGAGGGAGCTGGGCACCCTGAGGCGGGAGCGGGACCAGCTGGAAACCCAAACCAGAGAGTTGGAGACT GCCTACAGCAACCTCCTCCGAGACAAGTCAGTTCTGGAGGAAGAGAAGAAGCGACTAAGGCAAGAAAATG AGAATCTGGCCAGGAGGTTGGAAAGCAGCAGCCAGGAGGTAGCAAGGCTGAGAAGGGGCCAGTGTCCCCA GACCCGAGACACTGCTCGGGCTGTGCCACCAGGCTCCAGAGAAGTTTCTACGTGGAATTTGGACACTTTG GCCTTCCAGGAACTGAAGTCCGAGCTAACTGAAGTTCCTGCTTCCCGAATTTTGAAGGAGAGCCCATCTG GCTATCTCAGGAGTGGAGAGGGAGACACCGGATGTGGAGAACTAGTTTGGGTAGGAGAGCCTCTCACGCT GAGAACAGCAGAAACAATTACTGGCAAGTATGGTGTGTGGATGCGAGACCCCAAGCCCACCTACCCCTAC ACCCAGGAGACCACGTGGAGAATCGACACAGTTGGCACGGATGTCCGCCAGGTTTTTGAGTATGACCTCA TCAGCCAGTTTATGCAGGGCTACCCTTCTAAGGTTCACATACTGCCTAGGCCACTGGAAAGCACGGGTGC TGTGGTGTACTCGGGGAGCCTCTATTTCCAGGGCGCTGAGTCCAGAACTGTCATAAGATATGAGCTGAAT ACCGAGACAGTGAAGGCTGAGAAGGAAATCCCTGGAGCTGGCTACCACGGACAGTTCCCGTATTCTTGGG GTGGCTACACGGACATTGACTTGGCTGTGGATGAAGCAGGCCTCTGGGTCATTTACAGCACCGATGAGGC CAAAGGTGCCATTGTCCTCTCCAAACTGAACCCAGAGAATCTGGAACTCGAACAAACCTGGGAGACAAAC ATCCGTAAGCAGTCAGTCGCCAATGCCTTCATCATCTGTGGCACCTTGTACACCGTCAGCAGCTACACCT CAGCAGATGCTACCGTCAACTTTGCTTATGACACAGGCACAGGTATCAGCAAGACCCTGACCATCCCATT CAAGAACCGCTATAAGTACAGCAGCATGATTGACTACAACCCCCTGGAGAAGAAGCTCTTTGCCTGGGAC AACTTGAACATGGTCACTTATGACATCAAGCTCTCCAAGATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206556 representing NM_000261
Red=Cloning site Green=Tags(s) MRFFCARCCSFGPEMPAVQLLLLACLVWDVGARTAQLRKANDQSGRCQYTFSVASPNESSCPEQSQAMSV IHNLQRDSSTQRLDLEATKARLSSLESLLHQLTLDQAARPQETQEGLQRELGTLRRERDQLETQTRELET AYSNLLRDKSVLEEEKKRLRQENENLARRLESSSQEVARLRRGQCPQTRDTARAVPPGSREVSTWNLDTL AFQELKSELTEVPASRILKESPSGYLRSGEGDTGCGELVWVGEPLTLRTAETITGKYGVWMRDPKPTYPY TQETTWRIDTVGTDVRQVFEYDLISQFMQGYPSKVHILPRPLESTGAVVYSGSLYFQGAESRTVIRYELN TETVKAEKEIPGAGYHGQFPYSWGGYTDIDLAVDEAGLWVIYSTDEAKGAIVLSKLNPENLELEQTWETN IRKQSVANAFIICGTLYTVSSYTSADATVNFAYDTGTGISKTLTIPFKNRYKYSSMIDYNPLEKKLFAWD NLNMVTYDIKLSKM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_000261 |
ORF Size | 1512 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000261.2 |
RefSeq Size | 2061 bp |
RefSeq ORF | 1515 bp |
Locus ID | 4653 |
UniProt ID | Q99972 |
Protein Families | Druggable Genome, Secreted Protein |
MW | 56.8 kDa |
Gene Summary | MYOC encodes the protein myocilin, which is believed to have a role in cytoskeletal function. MYOC is expressed in many occular tissues, including the trabecular meshwork, and was revealed to be the trabecular meshwork glucocorticoid-inducible response protein (TIGR). The trabecular meshwork is a specialized eye tissue essential in regulating intraocular pressure, and mutations in MYOC have been identified as the cause of hereditary juvenile-onset open-angle glaucoma. [provided by RefSeq, Jul 2008] |
Citations (5)
The use of this cDNA Clones has been cited in the following citations: |
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Cross-talk between MYOC p. Y437H mutation and TGF-β2 in the pathology of glaucoma
,Yang, Y;Abdulatef, A;Zhang, L;Jiang, H;Zeng, Z;Li, H;Xia, X;,
Int. J. Med. Sci.
,PubMed ID 32410836
[Myocilin]
|
Role of truncated oxidized phospholipids in acute endothelial barrier dysfunction caused by particulate matter
,Karki, P;Meliton, A;Shah, A;Tian, Y;Ohmura, T;Sarich, N;Birukova, AA;Birukov, KG;,
PLoS ONE
,PubMed ID 30419037
[Myocilin]
|
Mutant myocilin impacts sarcomere ultrastructure in mouse gastrocnemius muscle
,Lynch, JM;Dolman, AJ;Guo, C;Dolan, K;Xiang, C;Reda, S;Li, B;Prasanna, G;,
PLoS ONE
,PubMed ID 30395621
[Myocilin]
|
Full-length myocilin protein is purified from mammalian cells as a dimer
,Katoli, P;Godbole, A;Romanowski, MJ;Clark, K;Meredith, E;Saenz-Vash, V;Wang, YK;Lewicki, N;Nguyen, AA;Lynch, JM;,
Protein Expr. Purif.
,PubMed ID 29475084
[Myocilin]
|
Glaucomatous MYOC mutations activate the IL-1/NF-?B inflammatory stress response and the glaucoma marker SELE in trabecular meshwork cells
,Itakura, T;Peters, DM;Fini, ME;,
Mol. Vis.
,PubMed ID 26396484
[Myocilin]
|
Documents
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FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC206556L1 | Lenti ORF clone of Human myocilin, trabecular meshwork inducible glucocorticoid response (MYOC), Myc-DDK-tagged |
CNY 8,032.00 |
|
RC206556L2 | Lenti ORF clone of Human myocilin, trabecular meshwork inducible glucocorticoid response (MYOC), mGFP tagged |
CNY 6,460.00 |
|
RC206556L3 | Lenti ORF clone of Human myocilin, trabecular meshwork inducible glucocorticoid response (MYOC), Myc-DDK-tagged |
CNY 6,460.00 |
|
RC206556L4 | Lenti ORF clone of Human myocilin, trabecular meshwork inducible glucocorticoid response (MYOC), mGFP tagged |
CNY 6,460.00 |
|
RG206556 | MYOC (tGFP-tagged) - Human myocilin, trabecular meshwork inducible glucocorticoid response (MYOC) |
CNY 7,232.00 |
|
SC122555 | MYOC (untagged)-Human myocilin, trabecular meshwork inducible glucocorticoid response (MYOC) |
CNY 5,640.00 |
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