CD5 (NM_014207) Human Tagged ORF Clone

CAT#: RC206494

CD5 (Myc-DDK-tagged)-Human CD5 molecule (CD5)

ORF Plasmid: DDK tGFP



  "NM_014207" in other vectors (6)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


CD5 mouse monoclonal antibody, clone OTI10F4 (formerly 10F4)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms LEU1; T1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC206494 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCATGGGGTCTCTGCAACCGCTGGCCACCTTGTACCTGCTGGGGATGCTGGTCGCTTCCTGCCTCG
GACGGCTCAGCTGGTATGACCCAGATTTCCAGGCAAGGCTCACCCGTTCCAACTCGAAGTGCCAGGGCCA
GCTGGAGGTCTACCTCAAGGACGGATGGCACATGGTTTGCAGCCAGAGCTGGGGCCGGAGCTCCAAGCAG
TGGGAGGACCCCAGTCAAGCGTCAAAAGTCTGCCAGCGGCTGAACTGTGGGGTGCCCTTAAGCCTTGGCC
CCTTCCTTGTCACCTACACACCTCAGAGCTCAATCATCTGCTACGGACAACTGGGCTCCTTCTCCAACTG
CAGCCACAGCAGAAATGACATGTGTCACTCTCTGGGCCTGACCTGCTTAGAACCCCAGAAGACAACACCT
CCAACGACAAGGCCCCCGCCCACCACAACTCCAGAGCCCACAGCTCCTCCCAGGCTGCAGCTGGTGGCAC
AGTCTGGCGGCCAGCACTGTGCCGGCGTGGTGGAGTTCTACAGCGGCAGCCTGGGGGGTACCATCAGCTA
TGAGGCCCAGGACAAGACCCAGGACCTGGAGAACTTCCTCTGCAACAACCTCCAGTGTGGCTCCTTCTTG
AAGCATCTGCCAGAGACTGAGGCAGGCAGAGCCCAAGACCCAGGGGAGCCACGGGAACACCAGCCCTTGC
CAATCCAATGGAAGATCCAGAACTCAAGCTGTACCTCCCTGGAGCATTGCTTCAGGAAAATCAAGCCCCA
GAAAAGTGGCCGAGTTCTTGCCCTCCTTTGCTCAGGTTTCCAGCCCAAGGTGCAGAGCCGTCTGGTGGGG
GGCAGCAGCATCTGTGAAGGCACCGTGGAGGTGCGCCAGGGGGCTCAGTGGGCAGCCCTGTGTGACAGCT
CTTCAGCCAGGAGCTCGCTGCGGTGGGAGGAGGTGTGCCGGGAGCAGCAGTGTGGCAGCGTCAACTCCTA
TCGAGTGCTGGACGCTGGTGACCCAACATCCCGGGGGCTCTTCTGTCCCCATCAGAAGCTGTCCCAGTGC
CACGAACTTTGGGAGAGAAATTCCTACTGCAAGAAGGTGTTTGTCACATGCCAGGATCCAAACCCCGCAG
GCCTGGCCGCAGGCACGGTGGCAAGCATCATCCTGGCCCTGGTGCTCCTGGTGGTGCTGCTGGTCGTGTG
CGGCCCCCTTGCCTACAAGAAGCTAGTGAAGAAATTCCGCCAGAAGAAGCAGCGCCAGTGGATTGGCCCA
ACGGGAATGAACCAAAACATGTCTTTCCATCGCAACCACACGGCAACCGTCCGATCCCATGCTGAGAACC
CCACAGCCTCCCACGTGGATAACGAATACAGCCAACCTCCCAGGAACTCCCGCCTGTCAGCTTATCCAGC
TCTGGAAGGGGTTCTGCATCGCTCCTCCATGCAGCCTGACAACTCCTCCGACAGTGACTATGATCTGCAT
GGGGCTCAGAGGCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC206494 protein sequence
Red=Cloning site Green=Tags(s)

MPMGSLQPLATLYLLGMLVASCLGRLSWYDPDFQARLTRSNSKCQGQLEVYLKDGWHMVCSQSWGRSSKQ
WEDPSQASKVCQRLNCGVPLSLGPFLVTYTPQSSIICYGQLGSFSNCSHSRNDMCHSLGLTCLEPQKTTP
PTTRPPPTTTPEPTAPPRLQLVAQSGGQHCAGVVEFYSGSLGGTISYEAQDKTQDLENFLCNNLQCGSFL
KHLPETEAGRAQDPGEPREHQPLPIQWKIQNSSCTSLEHCFRKIKPQKSGRVLALLCSGFQPKVQSRLVG
GSSICEGTVEVRQGAQWAALCDSSSARSSLRWEEVCREQQCGSVNSYRVLDAGDPTSRGLFCPHQKLSQC
HELWERNSYCKKVFVTCQDPNPAGLAAGTVASIILALVLLVVLLVVCGPLAYKKLVKKFRQKKQRQWIGP
TGMNQNMSFHRNHTATVRSHAENPTASHVDNEYSQPPRNSRLSAYPALEGVLHRSSMQPDNSSDSDYDLH
GAQRL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_014207
ORF Size 1485 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_014207.2, NP_055022.1
RefSeq Size 3180 bp
RefSeq ORF 1488 bp
Locus ID 921
UniProt ID P06127
Protein Families Druggable Genome, Transmembrane
Protein Pathways Hematopoietic cell lineage
MW 54.6 kDa
Gene Summary This gene encodes a member of the scavenger receptor cysteine-rich (SRCR) superfamily. Members of this family are secreted or membrane-anchored proteins mainly found in cells associated with the immune system. This protein is a type-I transmembrane glycoprotein found on the surface of thymocytes, T lymphocytes and a subset of B lymphocytes. The encoded protein contains three SRCR domains and may act as a receptor to regulate T-cell proliferation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2016]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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