Dynamin 1 (DNM1) (NM_001005336) Human Tagged ORF Clone
CAT#: RC206284
DNM1 (Myc-DDK-tagged)-Human dynamin 1 (DNM1), transcript variant 2
ORF Plasmid: tGFP
"NM_001005336" in other vectors (6)
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CNY 9,344.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | DEE31; DNM; EIEE31 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC206284 representing NM_001005336
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAACCGCGGCATGGAAGATCTCATCCCGCTGGTCAACCGGCTGCAAGACGCCTTCTCTGCCATCG GCCAGAACGCGGACCTCGACCTGCCGCAGATCGCTGTGGTGGGCGGCCAGAGCGCCGGCAAGAGCTCGGT GCTCGAGAATTTCGTAGGCAGGGACTTCTTGCCTCGAGGATCTGGCATTGTCACCCGACGTCCCCTGGTC TTGCAGCTGGTCAATGCAACCACAGAATATGCCGAGTTCCTGCACTGCAAGGGAAAGAAATTCACCGACT TCGAGGAGGTGCGCCTTGAGATCGAGGCCGAGACCGACAGGGTCACCGGCACCAACAAGGGCATCTCGCC GGTGCCTATCAACCTCCGCGTCTACTCGCCGCACGTGCTGAACCTGACCCTGGTGGACCTGCCCGGAATG ACCAAGGTCCCGGTGGGGGACCAACCTCCCGACATCGAGTTCCAGATCCGAGACATGCTTATGCAGTTTG TCACCAAGGAGAACTGCCTCATCCTGGCCGTGTCCCCCGCCAACTCTGACCTGGCCAATTCTGACGCCCT CGAGGTCGCCAAGGAGGTGGACCCCCAGGGCCAGCGCACCATCGGGGTCATCACCAAGCTGGACCTGATG GACGAGGGCACAGATGCCCGTGATGTGCTGGAGAACAAGCTGCTCCCCCTGCGCAGAGGCTACATTGGAG TGGTGAACCGGAGCCAGAAGGACATTGATGGCAAGAAGGACATTACCGCCGCCTTGGCTGCTGAACGAAA GTTCTTCCTCTCCCATCCATCTTATCGCCACTTGGCTGACCGTATGGGCACGCCCTACCTGCAGAAGGTC CTCAATCAGCAACTGACGGACCACATCCGGGACACACTGCCGGGGCTGCGGAACAAGCTGCAGAGCCAGC TACTGTCCATTGAGAAGGAGGTGGAGGAATACAAGAACTTCCGCCCTGATGACCCAGCTCGCAAGACCAA GGCCCTGCTGCAGATGGTCCAGCAGTTCGCCGTAGACTTTGAGAAGCGCATTGAGGGCTCAGGAGATCAG ATCGACACCTACGAACTGTCAGGGGGAGCCCGCATTAACCGAATCTTCCACGAGCGCTTCCCTTTCGAGC TGGTCAAGATGGAGTTTGATGAGAAGGAACTCCGAAGGGAGATCAGCTATGCTATCAAGAATATCCATGG CATTAGAACGGGGCTGTTTACCCCAGACATGGCCTTTGAGACCATTGTGAAAAAGCAGGTGAAGAAGATC CGAGAACCGTGTCTCAAGTGTGTGGACATGGTTATCTCGGAGCTAATCAGCACCGTTAGACAGTGCACCA AGAAGCTCCAGCAGTACCCGCGGCTACGGGAGGAGATGGAGCGCATCGTGACCACCCACATCCGGGAGCG CGAGGGCCGCACTAAGGAGCAGGTCATGCTTCTCATCGATATCGAGCTGGCTTACATGAACACCAACCAT GAGGACTTCATAGGCTTTGCCAATGCTCAGCAGAGGAGCAACCAGATGAACAAGAAGAAGACTTCAGGGA ACCAGGATGAGATTCTGGTCATCCGCAAGGGCTGGCTGACTATCAATAATATTGGCATCATGAAAGGGGG CTCCAAGGAGTACTGGTTTGTGCTGACTGCTGAGAATCTGTCCTGGTACAAGGATGATGAGGAGAAAGAG AAGAAATACATGCTGTCTGTGGACAACCTCAAGCTGCGGGACGTGGAGAAGGGCTTTATGTCGAGCAAGC ATATCTTTGCCCTCTTTAACACGGAGCAGAGGAATGTCTACAAGGATTATCGGCAGCTGGAGCTAGCCTG TGAGACACAGGAGGAGGTGGACAGCTGGAAGGCCTCCTTCCTGAGGGCTGGCGTGTACCCTGAGCGTGTT GGGGACAAAGAGAAAGCCAGCGAGACCGAGGAGAATGGCTCCGACAGCTTCATGCATTCCATGGACCCAC AGCTGGAACGGCAAGTGGAGACCATCCGGAATCTTGTGGACTCATACATGGCCATTGTCAACAAGACCGT GAGGGACCTCATGCCCAAGACCATCATGCACCTCATGATTAACAATACCAAGGAGTTCATCTTCTCGGAG CTGCTGGCCAACCTGTACTCGTGTGGGGACCAGAACACGCTGATGGAGGAGTCGGCGGAGCAGGCACAGC GGCGCGACGAGATGCTGCGCATGTACCACGCACTGAAGGAGGCGCTCAGCATCATCGGCGACATCAACAC GACCACCGTCAGCACGCCCATGCCCCCGCCCGTGGACGACTCCTGGCTGCAGGTGCAGAGCGTACCGGCC GGACGCAGGTCGCCCACGTCCAGCCCCACGCCGCAGCGCCGAGCCCCCGCCGTGCCCCCAGCCCGGCCCG GGTCGCGGGGCCCTGCTCCTGGGCCTCCGCCTGCTGGGTCCGCCATGGGGGGGGCGCCCCCCGTGCCCTC CAGGCCGGGGGCTTCCCCTGACCCTTTCGGCCCTCCCCCTCAGGTGCCCTCGCGCCCCAACCGCGCCCCG CCCGGGGTCCCCAGAATCACTATCAGTGACCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206284 representing NM_001005336
Red=Cloning site Green=Tags(s) MGNRGMEDLIPLVNRLQDAFSAIGQNADLDLPQIAVVGGQSAGKSSVLENFVGRDFLPRGSGIVTRRPLV LQLVNATTEYAEFLHCKGKKFTDFEEVRLEIEAETDRVTGTNKGISPVPINLRVYSPHVLNLTLVDLPGM TKVPVGDQPPDIEFQIRDMLMQFVTKENCLILAVSPANSDLANSDALEVAKEVDPQGQRTIGVITKLDLM DEGTDARDVLENKLLPLRRGYIGVVNRSQKDIDGKKDITAALAAERKFFLSHPSYRHLADRMGTPYLQKV LNQQLTDHIRDTLPGLRNKLQSQLLSIEKEVEEYKNFRPDDPARKTKALLQMVQQFAVDFEKRIEGSGDQ IDTYELSGGARINRIFHERFPFELVKMEFDEKELRREISYAIKNIHGIRTGLFTPDMAFETIVKKQVKKI REPCLKCVDMVISELISTVRQCTKKLQQYPRLREEMERIVTTHIREREGRTKEQVMLLIDIELAYMNTNH EDFIGFANAQQRSNQMNKKKTSGNQDEILVIRKGWLTINNIGIMKGGSKEYWFVLTAENLSWYKDDEEKE KKYMLSVDNLKLRDVEKGFMSSKHIFALFNTEQRNVYKDYRQLELACETQEEVDSWKASFLRAGVYPERV GDKEKASETEENGSDSFMHSMDPQLERQVETIRNLVDSYMAIVNKTVRDLMPKTIMHLMINNTKEFIFSE LLANLYSCGDQNTLMEESAEQAQRRDEMLRMYHALKEALSIIGDINTTTVSTPMPPPVDDSWLQVQSVPA GRRSPTSSPTPQRRAPAVPPARPGSRGPAPGPPPAGSAMGGAPPVPSRPGASPDPFGPPPQVPSRPNRAP PGVPRITISDP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001005336 |
ORF Size | 2553 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001005336.3 |
RefSeq Size | 3253 bp |
RefSeq ORF | 2556 bp |
Locus ID | 1759 |
UniProt ID | Q05193 |
Protein Families | Druggable Genome |
Protein Pathways | Endocytosis, Fc gamma R-mediated phagocytosis |
MW | 95.9 kDa |
Gene Summary | This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the encoded protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC206284L1 | Lenti ORF clone of Human dynamin 1 (DNM1), transcript variant 2, Myc-DDK-tagged |
CNY 11,744.00 |
|
RC206284L2 | Lenti ORF clone of Human dynamin 1 (DNM1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RC206284L3 | Lenti ORF clone of Human dynamin 1 (DNM1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC206284L4 | Lenti ORF clone of Human dynamin 1 (DNM1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RG206284 | DNM1 (tGFP-tagged) - Human dynamin 1 (DNM1), transcript variant 2 |
CNY 4,370.00 |
|
SC300909 | DNM1 (untagged)-Human dynamin 1 (DNM1), transcript variant 2 |
CNY 9,360.00 |
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