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SCAP2 (SKAP2) (NM_003930) Human Tagged ORF Clone
CAT＃: RC206119
SKAP2 (Myc-DDK-tagged)-Human src kinase associated phosphoprotein 2 (SKAP2)
ORF Plasmid: tGFP
"NM_003930" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PRAP; RA70; SAPS; SCAP2; SKAP-HOM; SKAP55R |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206119 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCAACCCCAGCAGCACCTCCTCTCCCTACCCCCTCCCTGAGGAAATTAGGAACCTGTTGGCAGATG TTGAAACATTTGTAGCAGATATACTGAAAGGAGAAAATTTATCCAAGAAAGCAAAGGAAAAGAGAGAATC CCTTATTAAGAAGATAAAAGATGTAAAGTCTATCTATCTTCAGGAATTTCAAGACAAAGGTGATGCAGAA GATGGGGAAGAATATGATGACCCTTTTGCTGGGCCTCCAGACACTATTTCATTAGCCTCAGAACGATATG ATAAAGACGATGAAGCCCCCTCTGATGGAGCCCAGTTTCCTCCAATTGCAGCACAAGACCTTCCTTTTGT TCTAAAGGCTGGCTACCTTGAAAAACGCAGAAAAGATCACAGCTTTCTGGGATTTGAATGGCAGAAACGG TGGTGTGCTCTCAGTAAAACGGTATTCTATTATTATGGAAGTGATAAAGACAAACAACAGAAAGGTGAAT TTGCAATAGATGGCTACAGTGTCAGAATGAATAACACTCTAAGAAAGGATGGAAAGAAAGATTGCTGTTT TGAAATCTCTGCTCCTGATAAACGTATATATCAGTTTACAGCAGCTTCTCCCAAAGATGCTGAAGAATGG GTACAGCAGCTGAAATTTGTATTGCAAGATATGGAATCTGATATTATTCCTGAGGATTATGATGAGAGAG GAGAATTATATGATGATGTTGATCATCCTCTACCAATAAGCAATCCACTAACAAGCAGTCAACCAATAGA TGATGAAATTTATGAAGAACTTCCAGAAGAAGAAGAGGACAGTGCTCCAGTGAAAGTGGAAGAACAAAGG AAGATGAGTCAGGATAGTGTCCATCACACCTCAGGGGATAAGAGCACTGATTATGCTAATTTTTACCAGG GATTGTGGGATTGTACTGGAGCTTTTTCTGATGAGTTGTCATTTAAGCGTGGTGATGTGATTTACATTCT TAGCAAGGAATACAATAGATATGGCTGGTGGGTAGGAGAAATGAAGGGAGCCATTGGCTTGGTGCCTAAA GCCTACATAATGGAGATGTATGATATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206119 protein sequence
Red=Cloning site Green=Tags(s) MPNPSSTSSPYPLPEEIRNLLADVETFVADILKGENLSKKAKEKRESLIKKIKDVKSIYLQEFQDKGDAE DGEEYDDPFAGPPDTISLASERYDKDDEAPSDGAQFPPIAAQDLPFVLKAGYLEKRRKDHSFLGFEWQKR WCALSKTVFYYYGSDKDKQQKGEFAIDGYSVRMNNTLRKDGKKDCCFEISAPDKRIYQFTAASPKDAEEW VQQLKFVLQDMESDIIPEDYDERGELYDDVDHPLPISNPLTSSQPIDDEIYEELPEEEEDSAPVKVEEQR KMSQDSVHHTSGDKSTDYANFYQGLWDCTGAFSDELSFKRGDVIYILSKEYNRYGWWVGEMKGAIGLVPK AYIMEMYDI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003930 |
| ORF Size | 1077 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003930.5 |
| RefSeq Size | 3984 bp |
| RefSeq ORF | 1080 bp |
| Locus ID | 8935 |
| UniProt ID | O75563 |
| Domains | SH3, PH |
| MW | 41.2 kDa |
| Gene Summary | The protein encoded by this gene shares homology with Src kinase-associated phosphoprotein 1, and is a substrate of Src family kinases. It is an adaptor protein that is thought to play an essential role in the Src signaling pathway, and in regulating proper activation of the immune system. This protein contains an amino terminal coiled-coil domain for self-dimerization, a plecskstrin homology (PH) domain required for interactions with lipids at the membrane, and a Src homology (SH3) domain at the carboxy terminus. Some reports indicate that this protein inhibits actin polymerization through interactions with actin assembly factors, and might negatively regulate the invasiveness of tumors by modulating actin assembly. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206119L1 | Lenti ORF clone of Human src kinase associated phosphoprotein 2 (SKAP2), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC206119L2 | Lenti ORF clone of Human src kinase associated phosphoprotein 2 (SKAP2), mGFP tagged |
CNY 5,890.00 |
|
| RC206119L3 | Lenti ORF clone of Human src kinase associated phosphoprotein 2 (SKAP2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206119L4 | Lenti ORF clone of Human src kinase associated phosphoprotein 2 (SKAP2), mGFP tagged |
CNY 5,890.00 |
|
| RG206119 | SKAP2 (tGFP-tagged) - Human src kinase associated phosphoprotein 2 (SKAP2) |
CNY 5,256.00 |
|
| SC117697 | SKAP2 (untagged)-Human src kinase associated phosphoprotein 2 (SKAP2) |
CNY 3,656.00 |
