PSCDBP (CYTIP) (NM_004288) Human Tagged ORF Clone

CAT#: RC206002

CYTIP (Myc-DDK-tagged)-Human cytohesin 1 interacting protein (CYTIP)

ORF Plasmid: DDK tGFP



  "NM_004288" in other vectors (4)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


CYTIP rabbit polyclonal antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms B3-1; CASP; CYBR; CYTHIP; HE; PSCDBP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC206002 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTTTACAAAGGCTCCTGCAACACAGCAGCAATGGCAATTTGGCGGACTTCTGCGCTGGGCCAGCGT
ATAGCTCTTACTCCACACTCACCGGCAGCCTTACGATGGACGATAATAGAAGGATTCAAATGCTAGCAGA
CACGGTGGCTACTCTGCCTCGGGGACGAAAGCAGCTTGCTTTGACCAGATCAAGTTCTTTAAGTGACTTT
TCCTGGTCTCAAAGAAAGCTTGTTACTGTGGAGAAGCAGGATAATGAAACATTTGGATTTGAAATTCAGT
CTTACAGGCCCCAGAATCAGAATGCCTGCTCCTCGGAAATGTTCACTTTGATATGCAAAATACAGGAGGA
CAGCCCAGCTCACTGTGCTGGCCTGCAAGCTGGTGATGTCCTTGCAAATATCAATGGTGTGAGCACAGAA
GGTTTTACCTACAAACAAGTCGTTGACCTGATCAGATCGTCCGGAAACCTGCTAACGATAGAGACTCTTA
ATGGAACAATGATTCTGAAAAGAACGGAGCTTGAAGCAAAGCTGCAGGTTTTAAAGCAAACTTTGAAACA
AAAATGGGTGGAGTACAGATCTCTGCAGTTACAGGAACATCGTCTGCTTCATGGTGATGCAGCTAATTGC
CCCAGTTTGGAAAACATGGACTTGGATGAATTGTCTTTGTTTGGACCCCTGCCTGGGCCAGGCCCAGCCC
TTGTGGACCGGAATCGATTATCCAGTGAGAGCAGCTGTAAGAGCTGGCTGAGCTCCATGACGATGGACAG
TGAAGATGGCTACCAGACGTGTGTGTCTGAGGACTCCAGCAGGGGTGCCTTCAGTCGGCAGACGAGTACA
GATGATGAGTGCTTTATCCCCAAGGAGGGGGATGATTTTCTGAGGAGGTCATCTTCAAGGAGGAACCGGA
GCATCAGTAACACCAGCAGCGGATCCATGTCTCCCTTGTGGGAGGGCAACTTATCAAGCATGTTTGGGAC
CCTGCCCCGGAAGAGCAGAAAGGGAAGTGTCCGAAAGCAACTCTTGAAATTTATCCCTGGCCTTCATCGT
GCTGTGGAAGAGGAAGAAAGTCGCTTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC206002 protein sequence
Red=Cloning site Green=Tags(s)

MSLQRLLQHSSNGNLADFCAGPAYSSYSTLTGSLTMDDNRRIQMLADTVATLPRGRKQLALTRSSSLSDF
SWSQRKLVTVEKQDNETFGFEIQSYRPQNQNACSSEMFTLICKIQEDSPAHCAGLQAGDVLANINGVSTE
GFTYKQVVDLIRSSGNLLTIETLNGTMILKRTELEAKLQVLKQTLKQKWVEYRSLQLQEHRLLHGDAANC
PSLENMDLDELSLFGPLPGPGPALVDRNRLSSESSCKSWLSSMTMDSEDGYQTCVSEDSSRGAFSRQTST
DDECFIPKEGDDFLRRSSSRRNRSISNTSSGSMSPLWEGNLSSMFGTLPRKSRKGSVRKQLLKFIPGLHR
AVEEEESRF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_004288
ORF Size 1077 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_004288.5
RefSeq Size 2282 bp
RefSeq ORF 1080 bp
Locus ID 9595
UniProt ID O60759
Domains PDZ
MW 40 kDa
Gene Summary The protein encoded by this gene contains 2 leucine zipper domains and a putative C-terminal nuclear targeting signal, but does not have any hydrophobic regions. This protein is expressed weakly in resting NK and T cells. The encoded protein modulates the activation of ARF genes by CYTH1. This protein interacts with CYTH1 and SNX27 proteins and may act to sequester CYTH1 protein in the cytoplasm.[provided by RefSeq, Aug 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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