RG9MTD1 (TRMT10C) (NM_017819) Human Tagged ORF Clone

CAT#: RC205903

TRMT10C (Myc-DDK-tagged)-Human RNA (guanine-9-) methyltransferase domain containing 1 (RG9MTD1), nuclear gene encoding mitochondrial protein

ORF Plasmid: DDK tGFP



  "NM_017819" in other vectors (7)

Need custom modification / cloning service?
Get a free quote

CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-RG9MTD1 Antibody
    • 100 ul

CNY 5,250.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms COXPD30; HNYA; MRPP1; RG9MTD1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC205903 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTGTTAGTGTCAATTTCTTCAGACCTTTCACCAGGTTTTTGGTGCCATTTACCCTTCATAGGAAGA
GAAATAACTTAACAATTTTGCAGAGATACATGTCTTCCAAAATACCAGCTGTTACTTATCCTAAAAATGA
GAGTACACCCCCTTCTGAAGAGCTAGAGTTGGATAAGTGGAAAACTACCATGAAATCTAGTGTGCAAGAA
GAATGTGTTTCAACAATCTCAAGCAGTAAGGATGAAGATCCTCTAGCTGCCACCAGAGAGTTCATTGAGA
TGTGGAGATTGCTTGGCAGAGAAGTACCAGAACACATCACTGAAGAAGAGCTCAAAACCCTTATGGAATG
TGTTTCTAACACAGCAAAAAAAAAATATTTAAAATATTTATATACGAAGGAAAAAGTGAAAAAAGCTAGG
CAAATAAAAAAGGAAATGAAAGCAGCAGCAAGGGAAGAAGCAAAAAATATCAAGCTGCTAGAAACCACTG
AGGAAGATAAACAGAAAAACTTTCTATTTTTACGACTTTGGGATAGGAATATGGACATAGCAATGGGCTG
GAAGGGTGCCCAGGCCATGCAGTTTGGACAACCTTTGGTTTTTGACATGGCTTACGAAAATTATATGAAA
CGAAAAGAATTGCAGAATACTGTTTCCCAGCTTTTAGAAAGTGAAGGATGGAACAGAAGAAATGTTGATC
CTTTCCATATTTATTTCTGCAATCTAAAAATAGATGGTGCTTTGCACAGAGAGTTAGTTAAACGGTATCA
AGAAAAATGGGACAAATTGCTTTTAACATCAACAGAAAAGTCTCATGTAGATTTATTTCCAAAGGACAGT
ATTATCTATTTAACTGCAGATTCTCCCAATGTTATGACTACTTTCAGGCATGACAAAGTTTATGTAATTG
GGTCTTTTGTTGATAAGAGTATGCAGCCAGGCACATCCCTAGCCAAGGCAAAACGGCTGAACCTGGCAAC
TGAATGCCTTCCATTAGATAAATATTTACAATGGGAAATTGGTAACAAAAATCTCACCTTAGATCAAATG
ATACGTATTTTGTTATGTCTGAAAAACAATGGTAATTGGCAAGAGGCTCTGCAATTCGTTCCCAAGAGAA
AACATACTGGTTTTCTGGAGATTTCTCAGCATTCTCAAGAGTTTATCAACAGACTAAAGAAGGCAAAGAC
T


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC205903 protein sequence
Red=Cloning site Green=Tags(s)

MSVSVNFFRPFTRFLVPFTLHRKRNNLTILQRYMSSKIPAVTYPKNESTPPSEELELDKWKTTMKSSVQE
ECVSTISSSKDEDPLAATREFIEMWRLLGREVPEHITEEELKTLMECVSNTAKKKYLKYLYTKEKVKKAR
QIKKEMKAAAREEAKNIKLLETTEEDKQKNFLFLRLWDRNMDIAMGWKGAQAMQFGQPLVFDMAYENYMK
RKELQNTVSQLLESEGWNRRNVDPFHIYFCNLKIDGALHRELVKRYQEKWDKLLLTSTEKSHVDLFPKDS
IIYLTADSPNVMTTFRHDKVYVIGSFVDKSMQPGTSLAKAKRLNLATECLPLDKYLQWEIGNKNLTLDQM
IRILLCLKNNGNWQEALQFVPKRKHTGFLEISQHSQEFINRLKKAKT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_017819
ORF Size 1191 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq Size 1861 bp
RefSeq ORF 1212 bp
Locus ID 54931
UniProt ID Q7L0Y3
Domains DUF425
MW 46.7 kDa
Gene Summary This gene encodes the precursor of a subunit of the mitochondrial ribonuclease P, which is involved in 5' processing of mitochondrial tRNAs. The encoded protein may confer RNA-binding capacity to mitochondrial ribonuclease P and may be essential for transcript processing, RNA modification, translation and mitochondrial respiration. [provided by RefSeq, Nov 2012]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...