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FBXW2 (NM_012164) Human Tagged ORF Clone
CAT＃: RC205840
FBXW2 (Myc-DDK-tagged)-Human F-box and WD repeat domain containing 2 (FBXW2)
ORF Plasmid: tGFP
"NM_012164" in other vectors (7)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | FBW2; Fwd2; Md6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205840 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAGAAAGGACTTTGAGACATGGCTTGATAACATTTCTGTTACATTTCTTTCTCTGACGGACTTGC AGAAAAATGAAACTCTGGATCACCTGATTAGTCTGAGTGGGGCAGTCCAGCTCAGGCATCTCTCCAATAA CCTAGAGACTCTCCTCAAGCGGGACTTCCTCAAACTCCTTCCCCTGGAGCTCAGTTTTTATTTGTTAAAA TGGCTCGATCCTCAGACTTTACTCACATGCTGCCTCGTCTCTAAACAGTGGAATAAGGTGATAAGTGCCT GTACAGAGGTGTGGCAGACTGCATGTAAAAATTTGGGCTGGCAGATAGATGATTCTGTTCAGGACGCTTT GCACTGGAAGAAGGTTTATTTGAAGGCTATTTTGAGAATGAAGCAACTGGAGGACCATGAAGCCTTTGAA ACCTCGTCATTAATTGGACACAGTGCCAGAGTGTATGCACTTTACTACAAAGATGGACTTCTCTGTACAG GGTCAGATGACTTGTCTGCAAAGCTGTGGGATGTGAGCACAGGGCAGTGCGTTTATGGCATCCAGACCCA CACTTGTGCAGCGGTGAAGTTTGATGAACAGAAGCTTGTGACAGGCTCCTTTGACAACACTGTGGCTTGC TGGGAATGGAGTTCCGGAGCCAGGACCCAGCACTTTCGGGGGCACACGGGGGCGGTATTTAGCGTGGACT ACAATGATGAACTGGATATCTTGGTGAGCGGCTCTGCAGACTTCACTGTGAAAGTATGGGCTTTATCTGC TGGGACATGCCTGAACACACTCACCGGGCACACGGAATGGGTCACCAAGGTAGTTTTGCAGAAGTGCAAA GTCAAGTCTCTCTTGCACAGTCCTGGAGACTACATCCTCTTAAGTGCAGACAAATATGAGATTAAGATTT GGCCAATTGGGAGAGAAATCAACTGTAAGTGCTTAAAGACATTGTCTGTCTCTGAGGATAGAAGTATCTG CCTGCAGCCAAGACTTCATTTTGATGGCAAATACATTGTCTGTAGTTCAGCACTTGGTCTCTACCAGTGG GACTTTGCCAGTTATGATATTCTCAGGGTCATCAAGACTCCTGAGATAGCAAACTTGGCCTTGCTTGGCT TTGGAGATATCTTTGCCCTGCTGTTTGACAACCGCTACCTGTACATCATGGACTTGCGGACAGAGAGCCT GATTAGTCGCTGGCCTCTGCCAGAGTACAGGAAATCAAAGAGAGGCTCAAGCTTCCTGGCAGGCGAAGCA TCCTGGCTGAATGGACTGGATGGGCACAATGACACGGGCTTGGTCTTTGCCACCAGCATGCCTGACCACA GTATTCACCTGGTGTTGTGGAAGGAGCACGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205840 protein sequence
Red=Cloning site Green=Tags(s) MERKDFETWLDNISVTFLSLTDLQKNETLDHLISLSGAVQLRHLSNNLETLLKRDFLKLLPLELSFYLLK WLDPQTLLTCCLVSKQWNKVISACTEVWQTACKNLGWQIDDSVQDALHWKKVYLKAILRMKQLEDHEAFE TSSLIGHSARVYALYYKDGLLCTGSDDLSAKLWDVSTGQCVYGIQTHTCAAVKFDEQKLVTGSFDNTVAC WEWSSGARTQHFRGHTGAVFSVDYNDELDILVSGSADFTVKVWALSAGTCLNTLTGHTEWVTKVVLQKCK VKSLLHSPGDYILLSADKYEIKIWPIGREINCKCLKTLSVSEDRSICLQPRLHFDGKYIVCSSALGLYQW DFASYDILRVIKTPEIANLALLGFGDIFALLFDNRYLYIMDLRTESLISRWPLPEYRKSKRGSSFLAGEA SWLNGLDGHNDTGLVFATSMPDHSIHLVLWKEHG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012164 |
| ORF Size | 1362 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_012164.4 |
| RefSeq Size | 9186 bp |
| RefSeq ORF | 1365 bp |
| Locus ID | 26190 |
| UniProt ID | Q9UKT8 |
| Domains | WD40, F-box |
| Protein Families | Druggable Genome |
| MW | 51.5 kDa |
| Gene Summary | F-box proteins are an expanding family of eukaryotic proteins characterized by an approximately 40 amino acid motif, the F box. Some F-box proteins have been shown to be critical for the ubiquitin-mediated degradation of cellular regulatory proteins. In fact, F-box proteins are one of the four subunits of ubiquitin protein ligases, called SCFs. SCF ligases bring ubiquitin conjugating enzymes to substrates that are specifically recruited by the different F-box proteins. Mammalian F-box proteins are classified into three groups based on the presence of either WD-40 repeats, leucine-rich repeats, or the presence or absence of other protein-protein interacting domains. This gene encodes the second identified member of the F-box gene family and contains multiple WD-40 repeats. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205840L1 | Lenti ORF clone of Human F-box and WD repeat domain containing 2 (FBXW2), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC205840L2 | Lenti ORF clone of Human F-box and WD repeat domain containing 2 (FBXW2), mGFP tagged |
CNY 5,890.00 |
|
| RC205840L3 | Lenti ORF clone of Human F-box and WD repeat domain containing 2 (FBXW2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205840L4 | Lenti ORF clone of Human F-box and WD repeat domain containing 2 (FBXW2), mGFP tagged |
CNY 5,890.00 |
|
| RG205840 | FBXW2 (tGFP-tagged) - Human F-box and WD repeat domain containing 2 (FBXW2) |
CNY 5,256.00 |
|
| SC115470 | FBXW2 (untagged)-Human F-box and WD repeat domain containing 2 (FBXW2) |
CNY 3,656.00 |
|
| SC317741 | FBXW2 (untagged)-Human F-box and WD repeat domain containing 2 (FBXW2) |
CNY 7,220.00 |
