THAP6 (NM_144721) Human Tagged ORF Clone

CAT#: RC205789

THAP6 (Myc-DDK-tagged)-Human THAP domain containing 6 (THAP6)

ORF Plasmid: DDK tGFP



  "NM_144721" in other vectors (4)

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CNY 2,400.00

CNY 3,705.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


THAP6 mouse monoclonal antibody, clone OTI1E10 (formerly 1E10)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC205789 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGAAATGCTGCTCCGCCATTGGATGTGCTTCTCGCTGCTTGCCAAATTCGAAGTTAAAAGGACTGA
CATTTCACGTATTCCCCACAGATGAAAACATCAAAAGGAAATGGGTATTAGCAATGAAAAGACTTGATGT
GAATGCAGCCGGCATTTGGGAGCCTAAAAAAGGAGATGTGTTGTGTTCGAGGCACTTTAAGAAGACAGAT
TTTGACAGAAGTGCTCCAAATATTAAACTGAAACCTGGAGTCATACCTTCTATCTTTGATTCTCCATATC
ACCTACAGGGGAAAAGAGAAAAACTTCATTGTAGAAAAAACTTCACCCTCAAAACCGTTCCAGCCACTAA
CTACAATCACCATCTTGTTGGTGCTTCCTCATGTATTGAAGAATTCCAATCCCAGTTCATTTTTGAACAT
AGCTACAGTGTAATGGACAGTCCAAAGAAACTTAAGCATAAATTAGATCATGTGATCGGCGAGCTAGAGG
ATACAAAGGAAAGTCTACGGAATGTTTTAGACCGAGAAAAACGTTTTCAGAAATCATTGAGGAAGACAAT
CAGGGAATTAAAGGATGAATGTCTGATCAGCCAAGAAACAGCAAATAGACTGGACACTTTCTGTTGGGAC
TGTTGTCAGGAGAGCATAGAACAGGACTATATTTCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC205789 protein sequence
Red=Cloning site Green=Tags(s)

MVKCCSAIGCASRCLPNSKLKGLTFHVFPTDENIKRKWVLAMKRLDVNAAGIWEPKKGDVLCSRHFKKTD
FDRSAPNIKLKPGVIPSIFDSPYHLQGKREKLHCRKNFTLKTVPATNYNHHLVGASSCIEEFQSQFIFEH
SYSVMDSPKKLKHKLDHVIGELEDTKESLRNVLDREKRFQKSLRKTIRELKDECLISQETANRLDTFCWD
CCQESIEQDYIS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_144721
ORF Size 666 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_144721.6
RefSeq Size 3578 bp
RefSeq ORF 669 bp
Locus ID 152815
UniProt ID Q8TBB0
MW 25.7 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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