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Adipose Triglyceride Lipase (PNPLA2) (NM_020376) Human Tagged ORF Clone
CAT＃: RC205708
PNPLA2 (Myc-DDK-tagged)-Human patatin-like phospholipase domain containing 2 (PNPLA2)
ORF Plasmid: tGFP
"NM_020376" in other vectors (7)
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CNY 3,752.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1110001C14Rik; ATGL; FP17548; iPLA2zeta; PEDF-R; TTS-2.2; TTS2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205708 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTCCCCGCGAGAAGACGTGGAACATCTCGTTCGCGGGCTGCGGCTTCCTCGGCGTCTACTACGTCG GCGTGGCCTCCTGCCTCCGCGAGCACGCGCCCTTCCTGGTGGCCAACGCCACGCACATCTACGGCGCCTC GGCCGGGGCGCTCACGGCCACGGCGCTGGTCACCGGGGTCTGCCTGGGTGAGGCTGGTGCCAAGTTCATT GAGGTATCTAAAGAGGCCCGGAAGCGGTTCCTGGGCCCCCTGCACCCCTCCTTCAACCTGGTAAAGATCA TCCGCAGTTTCCTGCTGAAGGTCCTGCCTGCTGATAGCCATGAGCATGCCAGTGGGCGCCTGGGCATCTC CCTGACCCGCGTGTCAGACGGCGAGAATGTCATTATATCCCACTTCAACTCCAAGGACGAGCTCATCCAG GCCAATGTCTGCAGCGGTTTCATCCCCGTGTACTGTGGGCTCATCCCTCCCTCCCTCCAGGGGGTGCGCT ACGTGGATGGTGGCATTTCAGACAACCTGCCACTCTATGAGCTTAAGAACACCATCACAGTGTCCCCCTT CTCGGGCGAGAGTGACATCTGTCCGCAGGACAGCTCCACCAACATCCACGAGCTGCGGGTCACCAACACC AGCATCCAGTTCAACCTGCGCAACCTCTACCGCCTCTCCAAGGCCCTCTTCCCGCCGGAGCCCCTGGTGC TGCGAGAGATGTGCAAGCAGGGATACCGGGATGGCCTGCGCTTTCTGCAGCGGAACGGCCTCCTGAACCG GCCCAACCCCTTGCTGGCGTTGCCCCCCGCCCGCCCCCACGGCCCAGAGGACAAGGACCAGGCAGTGGAG AGCGCCCAAGCGGAGGATTACTCGCAGCTGCCGGGAGAAGATCACATCCTGGAGCACCTGCCCGCCCGGC TCAATGAGGCCCTGCTGGAGGCCTGCGTGGAGCCCACGGACCTGCTGACCACCCTCTCCAACATGCTGCC TGTGCGTCTGGCCACGGCCATGATGGTGCCCTACACGCTGCCGCTGGAGAGCGCTCTGTCCTTCACCATC CGCTTGCTGGAGTGGCTGCCCGACGTTCCCGAGGACATCCGGTGGATGAAGGAGCAGACGGGCAGCATCT GCCAGTACCTGGTGATGCGCGCCAAGAGGAAGCTGGGCAGGCACCTGCCCTCCAGGCTGCCGGAGCAGGT GGAGCTGCGCCGCGTCCAGTCGCTGCCGTCCGTGCCGCTGTCCTGCGCCGCCTACAGAGAGGCACTGCCC GGCTGGATGCGCAACAACCTCTCGCTGGGGGACGCGCTGGCCAAGTGGGAGGAGTGCCAGCGCCAGCTGC TGCTCGGCCTCTTCTGCACCAACGTGGCCTTCCCGCCCGAAGCTCTGCGCATGCGCGCACCCGCCGACCC GGCTCCCGCCCCCGCGGACCCAGCATCCCCGCAGCACCAGCTGGCCGGGCCTGCCCCCTTGCTGAGCACC CCTGCTCCCGAGGCCCGGCCCGTGATCGGGGCCCTGGGGCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205708 protein sequence
Red=Cloning site Green=Tags(s) MFPREKTWNISFAGCGFLGVYYVGVASCLREHAPFLVANATHIYGASAGALTATALVTGVCLGEAGAKFI EVSKEARKRFLGPLHPSFNLVKIIRSFLLKVLPADSHEHASGRLGISLTRVSDGENVIISHFNSKDELIQ ANVCSGFIPVYCGLIPPSLQGVRYVDGGISDNLPLYELKNTITVSPFSGESDICPQDSSTNIHELRVTNT SIQFNLRNLYRLSKALFPPEPLVLREMCKQGYRDGLRFLQRNGLLNRPNPLLALPPARPHGPEDKDQAVE SAQAEDYSQLPGEDHILEHLPARLNEALLEACVEPTDLLTTLSNMLPVRLATAMMVPYTLPLESALSFTI RLLEWLPDVPEDIRWMKEQTGSICQYLVMRAKRKLGRHLPSRLPEQVELRRVQSLPSVPLSCAAYREALP GWMRNNLSLGDALAKWEECQRQLLLGLFCTNVAFPPEALRMRAPADPAPAPADPASPQHQLAGPAPLLST PAPEARPVIGALGL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020376 |
| ORF Size | 1512 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_020376.2, NP_065109.1 |
| RefSeq Size | 2443 bp |
| RefSeq ORF | 1515 bp |
| Locus ID | 57104 |
| UniProt ID | Q96AD5 |
| Protein Families | Transmembrane |
| MW | 55.3 kDa |
| Gene Summary | This gene encodes an enzyme which catalyzes the first step in the hydrolysis of triglycerides in adipose tissue. Mutations in this gene are associated with neutral lipid storage disease with myopathy. [provided by RefSeq, Jul 2010] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Peroxisomal β-oxidation acts as a sensor for intracellular fatty acids and regulates lipolysis
,null,
Nature Metabolism
,PubMed ID 34903883
[Adipose Triglyceride Lipase]
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Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205708L1 | Lenti ORF clone of Human patatin-like phospholipase domain containing 2 (PNPLA2), Myc-DDK-tagged |
CNY 6,152.00 |
|
| RC205708L2 | Lenti ORF clone of Human patatin-like phospholipase domain containing 2 (PNPLA2), mGFP tagged |
CNY 6,152.00 |
|
| RC205708L3 | Lenti ORF clone of Human patatin-like phospholipase domain containing 2 (PNPLA2), Myc-DDK-tagged |
CNY 6,152.00 |
|
| RC205708L4 | Lenti ORF clone of Human patatin-like phospholipase domain containing 2 (PNPLA2), mGFP tagged |
CNY 6,152.00 |
|
| RG205708 | PNPLA2 (tGFP-tagged) - Human patatin-like phospholipase domain containing 2 (PNPLA2) |
CNY 5,352.00 |
|
| SC107379 | PNPLA2 (untagged)-Human patatin-like phospholipase domain containing 2 (PNPLA2) |
CNY 3,760.00 |
|
| SC320436 | PNPLA2 (untagged)-Human patatin-like phospholipase domain containing 2 (PNPLA2) |
CNY 3,760.00 |
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