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ZNF165 (NM_003447) Human Tagged ORF Clone
CAT＃: RC205600
ZNF165 (Myc-DDK-tagged)-Human zinc finger protein 165 (ZNF165)
ORF Plasmid: tGFP
"NM_003447" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
| Cited in 3 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CT53; LD65; ZSCAN7 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205600 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTACAGAACCAAAGAAAGCTGCAGCCCAGAACTCTCCAGAGGATGAAGGACTTCTGATAGTGAAGA TAGAAGAGGAAGAATTTATCCATGGGCAGGACACTTGCTTACAGAGAAGTGAACTCCTTAAGCAGGAGCT CTGCAGGCAGCTTTTTAGGCAGTTCTGCTACCAGGATTCTCCTGGACCTCGCGAGGCACTGAGCCGCCTC CGGGAGCTCTGCTGTCAGTGGCTGAAGCCAGAGATCCATACCAAGGAACAGATTCTGGAACTGCTGGTGC TAGAGCAGTTCCTGACCATCCTGCCAGGAGATTTGCAGGCCTGGGTACATGAACATTACCCAGAGAGTGG AGAGGAGGCAGTGACCATACTAGAAGATTTGGAGAGAGGCACTGATGAAGCAGTACTCCAGGTTCAAGCC CATGAACATGGACAAGAAATATTCCAGAAAAAAGTGTCACCTCCTGGACCAGCACTTAATGTCAAGTTAC AGCCAGTGGAGACCAAGGCCCATTTTGATTCATCAGAACCCCAGCTCCTATGGGACTGTGATAATGAGAG TGAAAACAGTAGATCCATGCCAAAGCTGGAAATTTTTGAAAAAATTGAATCACAGAGAATTATATCTGGA AGAATCTCAGGATACATATCAGAAGCGTCTGGTGAGTCTCAAGACATCTGTAAGTCTGCAGGCAGGGTAA AGAGACAATGGGAAAAAGAATCAGGGGAGTCTCAGAGACTCTCGTCTGCCCAGGATGAAGGTTTTGGTAA AATCCTCACCCACAAAAATACAGTCAGAGGTGAAATAATAAGCCACGATGGATGTGAGAGGAGATTAAAT CTGAACTCAAATGAATTCACACACCAGAAATCTTGTAAACATGGTACCTGTGACCAGAGCTTCAAATGGA ACTCAGATTTTATTAACCATCAAATAATTTATGCTGGAGAAAAAAATCACCAATATGGAAAATCTTTCAA GAGCCCAAAACTTGCTAAACATGCAGCAGTTTTCAGTGGAGATAAAACTCATCAGTGTAATGAATGTGGG AAAGCTTTCAGGCACAGCTCAAAACTTGCTAGGCATCAGAGAATCCACACTGGAGAGAGATGCTATGAAT GTAATGAATGTGGGAAAAGCTTTGCAGAGAGCTCAGATCTTACTAGACATCGGCGAATTCACACTGGGGA AAGACCCTTTGGTTGCAAAGAATGTGGGAGAGCATTCAACCTGAACTCACATCTTATCAGGCATCAGAGA ATTCACACCAGAGAGAAACCCTACGAGTGTAGTGAATGTGGGAAAACCTTCCGAGTGAGCTCACATCTTA TTCGACACTTTAGAATTCACACTGGAGAAAAACCCTATGAATGCAGTGAGTGTGGAAGAGCCTTCAGTCA GAGCTCAAACCTTAGTCAACACCAGAGAATTCACATGAGGGAAAACCTATTAATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205600 protein sequence
Red=Cloning site Green=Tags(s) MATEPKKAAAQNSPEDEGLLIVKIEEEEFIHGQDTCLQRSELLKQELCRQLFRQFCYQDSPGPREALSRL RELCCQWLKPEIHTKEQILELLVLEQFLTILPGDLQAWVHEHYPESGEEAVTILEDLERGTDEAVLQVQA HEHGQEIFQKKVSPPGPALNVKLQPVETKAHFDSSEPQLLWDCDNESENSRSMPKLEIFEKIESQRIISG RISGYISEASGESQDICKSAGRVKRQWEKESGESQRLSSAQDEGFGKILTHKNTVRGEIISHDGCERRLN LNSNEFTHQKSCKHGTCDQSFKWNSDFINHQIIYAGEKNHQYGKSFKSPKLAKHAAVFSGDKTHQCNECG KAFRHSSKLARHQRIHTGERCYECNECGKSFAESSDLTRHRRIHTGERPFGCKECGRAFNLNSHLIRHQR IHTREKPYECSECGKTFRVSSHLIRHFRIHTGEKPYECSECGRAFSQSSNLSQHQRIHMRENLLM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003447 |
| ORF Size | 1455 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003447.4 |
| RefSeq Size | 2411 bp |
| RefSeq ORF | 1458 bp |
| Locus ID | 7718 |
| UniProt ID | P49910 |
| Protein Families | Stem cell - Pluripotency, Transcription Factors |
| MW | 55.8 kDa |
| Gene Summary | This gene encodes a member of the Kruppel family of zinc finger proteins. Members of this DNA-binding protein family act as transcriptional regulators. This gene is located within a cluster of zinc finger family members. The encoded protein may play a role in spermatogenesis. [provided by RefSeq, Jul 2008] |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The testis protein ZNF165 is a SMAD3 cofactor that coordinates oncogenic TGFβ signaling in triple-negative breast cancer
,Gibbs, ZA;Reza, LC;Cheng, CC;Westcott, JM;McGlynn, K;Whitehurst, AW;,
Elife
,PubMed ID 32515734
[ZNF165]
|
|
The testis protein ZNF165 is a SMAD3 cofactor that coordinates oncogenic TGF-beta; signaling in triple-negative breast cancer
,Gibbs, ZA;Reza, LC;Cheng, CC;Westcott, JM;,
bioRxiv
[ZNF165]
|
|
Comprehensive functional characterization of cancer-testis antigens defines obligate participation in multiple hallmarks of cancer
,Maxfield, KE;Taus, PJ;Corcoran, K;Wooten, J;Macion, J;Zhou, Y;Borromeo, M;Kollipara, RK;Yan, J;Xie, Y;Xie, XJ;Whitehurst, AW;,
Nat Commun
,PubMed ID 26567849
[ZNF165]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205600L1 | Lenti ORF clone of Human zinc finger protein 165 (ZNF165), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC205600L2 | Lenti ORF clone of Human zinc finger protein 165 (ZNF165), mGFP tagged |
CNY 5,890.00 |
|
| RC205600L3 | Lenti ORF clone of Human zinc finger protein 165 (ZNF165), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205600L4 | Lenti ORF clone of Human zinc finger protein 165 (ZNF165), mGFP tagged |
CNY 5,890.00 |
|
| RG205600 | ZNF165 (tGFP-tagged) - Human zinc finger protein 165 (ZNF165) |
CNY 5,256.00 |
|
| SC303307 | ZNF165 (untagged)-Human zinc finger protein 165 (ZNF165) |
CNY 3,656.00 |
