Carbohydrate sulfotransferase 1 (CHST1) (NM_003654) Human Tagged ORF Clone
CAT#: RC205588
CHST1 (Myc-DDK-tagged)-Human carbohydrate (keratan sulfate Gal-6) sulfotransferase 1 (CHST1)
ORF Plasmid: tGFP
"NM_003654" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | C6ST; GST-1; KS6ST; KSGal6ST; KSST |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC205588 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAATGTTCCTGGAAGGCCGTCCTCCTCCTTGCCCTGGCCTCCATTGCCATCCAGTACACGGCCATCC GCACCTTCACCGCCAAGTCCTTTCACACCTGCCCCGGGCTGGCAGAGGCCGGGCTGGCCGAGCGACTGTG CGAGGAGAGCCCCACCTTCGCCTACAACCTCTCCCGCAAGACCCACATCCTCATCCTGGCCACCACGCGC AGCGGCTCCTCCTTCGTGGGCCAGCTCTTCAACCAGCACCTGGACGTCTTCTACCTGTTTGAGCCCCTCT ACCACGTCCAGAACACGCTCATCCCCCGCTTCACCCAGGGCAAGAGCCCGGCCGACCGGCGGGTCATGCT AGGCGCCAGCCGCGACCTCCTGCGGAGCCTCTACGACTGCGACCTCTACTTCCTGGAGAACTACATCAAG CCGCCGCCGGTCAACCACACCACCGACAGGATCTTCCGCCGCGGGGCCAGCCGGGTCCTCTGCTCCCGGC CTGTGTGCGACCCTCCGGGGCCAGCCGACCTGGTCCTGGAGGAGGGGGACTGTGTGCGCAAGTGCGGGCT ACTCAACCTGACCGTGGCGGCCGAGGCGTGCCGCGAGCGCAGCCACGTGGCCATCAAGACGGTGCGCGTG CCCGAGGTGAACGACCTGCGCGCCCTGGTGGAAGACCCGCGATTAAACCTCAAGGTCATCCAGCTGGTCC GAGACCCCCGCGGCATTCTGGCTTCGCGCAGCGAGACCTTCCGCGACACGTACCGGCTCTGGCGGCTCTG GTACGGCACCGGGAGGAAACCCTACAACCTGGACGTGACGCAGCTGACCACGGTGTGCGAGGACTTCTCC AACTCCGTGTCCACCGGCCTCATGCGGCCCCCGTGGCTCAAGGGCAAGTACATGTTGGTGCGCTACGAGG ACCTGGCTCGGAACCCTATGAAGAAGACCGAGGAGATCTACGGGTTCCTGGGCATCCCGCTGGACAGCCA CGTGGCCCGCTGGATCCAGAACAACACGCGGGGCGACCCCACCCTGGGCAAGCACAAATACGGCACCGTG CGAAACTCGGCGGCCACGGCCGAGAAGTGGCGCTTCCGCCTCTCCTACGACATCGTGGCCTTTGCCCAGA ACGCCTGCCAGCAGGTGCTGGCCCAGCTGGGCTACAAGATCGCCGCCTCGGAGGAGGAGCTGAAGAACCC CTCGGTCAGCCTGGTGGAGGAGCGGGACTTCCGCCCCTTCTCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205588 protein sequence
Red=Cloning site Green=Tags(s) MQCSWKAVLLLALASIAIQYTAIRTFTAKSFHTCPGLAEAGLAERLCEESPTFAYNLSRKTHILILATTR SGSSFVGQLFNQHLDVFYLFEPLYHVQNTLIPRFTQGKSPADRRVMLGASRDLLRSLYDCDLYFLENYIK PPPVNHTTDRIFRRGASRVLCSRPVCDPPGPADLVLEEGDCVRKCGLLNLTVAAEACRERSHVAIKTVRV PEVNDLRALVEDPRLNLKVIQLVRDPRGILASRSETFRDTYRLWRLWYGTGRKPYNLDVTQLTTVCEDFS NSVSTGLMRPPWLKGKYMLVRYEDLARNPMKKTEEIYGFLGIPLDSHVARWIQNNTRGDPTLGKHKYGTV RNSAATAEKWRFRLSYDIVAFAQNACQQVLAQLGYKIAASEEELKNPSVSLVEERDFRPFS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_003654 |
ORF Size | 1233 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_003654.3 |
RefSeq Size | 3947 bp |
RefSeq ORF | 1236 bp |
Locus ID | 8534 |
UniProt ID | O43916 |
Domains | Sulfotransfer |
Protein Families | Transmembrane |
Protein Pathways | Keratan sulfate biosynthesis, Metabolic pathways |
MW | 46.7 kDa |
Gene Summary | This locus encodes a member of the keratin sulfotransferase family of proteins. The encoded enzyme catalyzes the sulfation of the proteoglycan keratin. [provided by RefSeq, Aug 2011] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC205588L3 | Lenti ORF clone of Human carbohydrate (keratan sulfate Gal-6) sulfotransferase 1 (CHST1), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC205588L4 | Lenti ORF clone of Human carbohydrate (keratan sulfate Gal-6) sulfotransferase 1 (CHST1), mGFP tagged |
CNY 5,890.00 |
|
RG205588 | CHST1 (tGFP-tagged) - Human carbohydrate (keratan sulfate Gal-6) sulfotransferase 1 (CHST1) |
CNY 5,256.00 |
|
SC117840 | CHST1 (untagged)-Human carbohydrate (keratan sulfate Gal-6) sulfotransferase 1 (CHST1) |
CNY 3,656.00 |
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