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CRISP2 (NM_003296) Human Tagged ORF Clone
CAT＃: RC205312
CRISP2 (Myc-DDK-tagged)-Human cysteine-rich secretory protein 2 (CRISP2), transcript variant 1
ORF Plasmid: tGFP
"NM_003296" in other vectors (6)
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CNY 2,400.00
CNY 3,705.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CRISP-2; CT36; GAPDL5; TPX1; TSP1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205312 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTTACTACCGGTGTTGTTTCTGGTTACTGTGCTGCTTCCATCTTTACCTGCAGAAGGAAAGGATC CCGCTTTTACTGCTTTGTTAACCACCCAGTTGCAAGTGCAAAGGGAGATTGTAAATAAACACAATGAACT AAGGAAAGCAGTCTCTCCACCTGCCAGTAACATGCTAAAGATGGAATGGAGCAGAGAGGTAACAACGAAT GCCCAAAGGTGGGCAAACAAGTGCACTTTACAACATAGTGATCCAGAGGACCGCAAAACCAGTACAAGAT GTGGTGAGAATCTCTATATGTCAAGTGACCCTACTTCCTGGTCTTCTGCAATCCAAAGCTGGTATGACGA GATCCTAGATTTTGTCTATGGTGTAGGACCAAAGAGTCCCAATGCAGTTGTTGGACATTATACTCAGCTT GTTTGGTACTCGACTTACCAGGTAGGCTGTGGAATTGCCTACTGTCCCAATCAAGATAGTCTAAAATACT ACTATGTTTGCCAATATTGTCCTGCTGGTAATAATATGAATAGAAAGAATACCCCGTACCAACAAGGAAC ACCTTGTGCCGGTTGCCCTGATGACTGTGACAAAGGACTATGCACCAATAGTTGCCAGTATCAAGATCTC CTAAGTAACTGTGATTCCTTGAAGAATACAGCTGGCTGTGAACATGAGTTACTCAAGGAAAAGTGCAAGG CTACTTGCCTATGTGAGAACAAAATTTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205312 protein sequence
Red=Cloning site Green=Tags(s) MALLPVLFLVTVLLPSLPAEGKDPAFTALLTTQLQVQREIVNKHNELRKAVSPPASNMLKMEWSREVTTN AQRWANKCTLQHSDPEDRKTSTRCGENLYMSSDPTSWSSAIQSWYDEILDFVYGVGPKSPNAVVGHYTQL VWYSTYQVGCGIAYCPNQDSLKYYYVCQYCPAGNNMNRKNTPYQQGTPCAGCPDDCDKGLCTNSCQYQDL LSNCDSLKNTAGCEHELLKEKCKATCLCENKIY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003296 |
| ORF Size | 729 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003296.4 |
| RefSeq Size | 1413 bp |
| RefSeq ORF | 732 bp |
| Locus ID | 7180 |
| UniProt ID | P16562 |
| Domains | SCP |
| Protein Families | Secreted Protein |
| MW | 27.3 kDa |
| Gene Summary | May regulate some ion channels' activity and therebye regulate calcium fluxes during sperm capacitation.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Mapping of the binding sites involved in PSP94-CRISP-3 interaction by molecular dissection of the complex.
,null,
Biochimica et biophysica acta
,PubMed ID 23375721
[CRISP2]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205312L1 | Lenti ORF clone of Human cysteine-rich secretory protein 2 (CRISP2), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205312L2 | Lenti ORF clone of Human cysteine-rich secretory protein 2 (CRISP2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC205312L3 | Lenti ORF clone of Human cysteine-rich secretory protein 2 (CRISP2), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205312L4 | Lenti ORF clone of Human cysteine-rich secretory protein 2 (CRISP2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG205312 | CRISP2 (tGFP-tagged) - Human cysteine-rich secretory protein 2 (CRISP2), transcript variant 1 |
CNY 4,000.00 |
|
| SC118057 | CRISP2 (untagged)-Human cysteine-rich secretory protein 2 (CRISP2), transcript variant 1 |
CNY 2,400.00 |
