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EPS8 (NM_004447) Human Tagged ORF Clone
CAT＃: RC205300
EPS8 (Myc-DDK-tagged)-Human epidermal growth factor receptor pathway substrate 8 (EPS8)
ORF Plasmid: tGFP
"NM_004447" in other vectors (6)
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CNY 9,032.00
Product images
CNY 300.00
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DFNB102 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205300 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGGTCATATTTCTAATCATCCCAGTAGTTTTGGAATGTACCCATCTCAGATGAATGGCTACGGAT CATCACCTACCTTTTCCCAGACGGACAGAGAACATGGTTCAAAAACAAGTGCAAAGGCCCTTTATGAACA AAGGAAGAATTATGCACGGGACAGTGTCAGCAGTGTGTCAGATATATCTCAATACCGTGTTGAACACTTG ACTACCTTTGTCCTGGATCGGAAAGATGCTATGATCACTGTTGATGATGGAATAAGGAAATTGAAATTGC TTGATGCCAAGGGCAAAGTGTGGACTCAAGATATGATTCTTCAAGTGGATGACAGAGCTGTGAGCCTGAT TGATTTAGAATCAAAGAATGAACTGGAGAATTTTCCTTTAAACACAATCCAGCACTGCCAAGCTGTGATG CATTCATGCAGCTATGATTCAGTTCTTGCACTGGTGTGCAAAGAGCCAACCCAGAACAAGCCAGATCTTC ATCTCTTCCAGTGTGATGAGGTTAAGGCAAACCTAATTAGTGAAGATATTGAAAGTGCAATCAGTGACAG TAAAGGAGGGAAACAGAAGAGGCGGCCCGACGCCCTGAGGATGATTTCCAATGCAGACCCTAGTATACCG CCTCCACCCAGAGCTCCTGCCCCTGCGCCCCCTGGGACCGTCACCCAGGTGGATGTTAGAAGTCGAGTGG CAGCCTGGTCTGCATGGGCAGCCGACCAAGGGGACTTTGAGAAACCAAGGCAGTATCATGAGCAGGAAGA AACACCTGAGATGATGGCAGCCCGCATTGACAGAGATGTGCAAATCTTAAACCACATTTTGGATGACATT GAATTTTTTATCACAAAACTCCAAAAAGCAGCAGAAGCATTTTCTGAGCTTTCTAAAAGGAAGAAAAACA AGAAAGGTAAAAGGAAAGGACCAGGAGAGGGTGTTTTAACGCTGCGGGCAAAACCTCCACCTCCTGATGA ATTTCTTGACTGTTTCCAAAAGTTTAAACACGGATTTAACCTTCTGGCCAAACTGAAGTCTCATATTCAG AATCCTAGTGCTGCAGATTTGGTTCACTTTTTGTTTACTCCATTAAATATGGTGGTGCAGGCAACAGGAG GTCCTGAACTAGCCAGTTCAGTACTTAGTCCCCTATTGAATAAGGACACAATTGATTTCTTAAATTATAC TGTCAATGGTGATGAACGGCAGCTGTGGATGTCATTGGGAGGAACTTGGATGAAAGCCAGAGCAGAGTGG CCAAAAGAACAGTTTATTCCACCATATGTTCCACGATTCCGCAATGGCTGGGAGCCCCCAATGCTGAACT TTATGGGAGCCACAATGGAACAAGATCTTTATCAACTGGCAGAATCTGTGGCAAATGTAGCAGAACATCA GCGCAAACAGGAAATAAAAAGATTATCCACAGAGCATTCCAGTGTATCAGAGTATCATCCAGCCGATGGC TATGCGTTCAGTAGCAACATTTACACAAGAGGATCCCACCTGGACCAAGGGGAAGCTGCTGTTGCTTTTA AGCCAACTTCTAATCGCCATATAGATAGAAATTATGAACCACTCAAAACACAACCCAAGAAATATGCCAA ATCCAAGTATGACTTTGTAGCAAGGAACAACAGTGAGCTCTCGGTTCTAAAGGATGATATTTTAGAGATA CTTGATGATCGGAAGCAATGGTGGAAAGTTCGAAATGCAAGTGGAGACTCTGGATTTGTGCCAAATAACA TTTTGGATATTGTGAGACCTCCAGAATCTGGATTGGGGCGTGCTGATCCACCTTATACTCATACTATACA GAAACAAAGGATGGAGTATGGCCCAAGACCAGCTGATACTCCCCCTGCTCCATCACCTCCTCCAACACCA GTTCCTGTTCCTGTTCCCCTTCCCCCTTCCACTCCAGCACCTGTTCCTGTGTCAAAGGTCCCAGCAAATA TAACACGTCAAAACAGCAGCTCCAGTGACAGTGGTGGCAGTATCGTGCGAGACAGCCAGAGACACAAACA ACTTCCGGTGGACCGAAGGAAATCTCAGATGGAGGAAGTGCAAGATGAACTCATCCACAGACTGACCATT GGTCGGAGTGCCGCTCAGAAGAAATTCCATGTGCCACGGCAGAACGTGCCAGTTATCAATATCACTTACG ACTCCACACCAGAGGATGTGAAGACGTGGTTACAGTCAAAGGGATTCAACCCTGTGACTGTCAATAGTCT TGGAGTATTAAATGGTGCACAACTTTTCTCTCTCAATAAGGATGAACTGAGGACAGTCTGCCCTGAAGGG GCGAGAGTCTATAGCCAAATCACTGTACAAAAAGCTGCATTGGAGGATAGCAGTGGCAGCTCCGAGTTAC AAGAAATTATGAGAAGACGACAGGAAAAAATCAGTGCTGCCGCTAGTGATTCAGGAGTGGAATCTTTTGA TGAAGGAAGCAGTCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205300 protein sequence
Red=Cloning site Green=Tags(s) MNGHISNHPSSFGMYPSQMNGYGSSPTFSQTDREHGSKTSAKALYEQRKNYARDSVSSVSDISQYRVEHL TTFVLDRKDAMITVDDGIRKLKLLDAKGKVWTQDMILQVDDRAVSLIDLESKNELENFPLNTIQHCQAVM HSCSYDSVLALVCKEPTQNKPDLHLFQCDEVKANLISEDIESAISDSKGGKQKRRPDALRMISNADPSIP PPPRAPAPAPPGTVTQVDVRSRVAAWSAWAADQGDFEKPRQYHEQEETPEMMAARIDRDVQILNHILDDI EFFITKLQKAAEAFSELSKRKKNKKGKRKGPGEGVLTLRAKPPPPDEFLDCFQKFKHGFNLLAKLKSHIQ NPSAADLVHFLFTPLNMVVQATGGPELASSVLSPLLNKDTIDFLNYTVNGDERQLWMSLGGTWMKARAEW PKEQFIPPYVPRFRNGWEPPMLNFMGATMEQDLYQLAESVANVAEHQRKQEIKRLSTEHSSVSEYHPADG YAFSSNIYTRGSHLDQGEAAVAFKPTSNRHIDRNYEPLKTQPKKYAKSKYDFVARNNSELSVLKDDILEI LDDRKQWWKVRNASGDSGFVPNNILDIVRPPESGLGRADPPYTHTIQKQRMEYGPRPADTPPAPSPPPTP VPVPVPLPPSTPAPVPVSKVPANITRQNSSSSDSGGSIVRDSQRHKQLPVDRRKSQMEEVQDELIHRLTI GRSAAQKKFHVPRQNVPVINITYDSTPEDVKTWLQSKGFNPVTVNSLGVLNGAQLFSLNKDELRTVCPEG ARVYSQITVQKAALEDSSGSSELQEIMRRRQEKISAAASDSGVESFDEGSSH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004447 |
| ORF Size | 2466 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004447.3 |
| RefSeq Size | 4088 bp |
| RefSeq ORF | 2469 bp |
| Locus ID | 2059 |
| UniProt ID | Q12929 |
| Domains | SH3, PID |
| Protein Families | Druggable Genome |
| MW | 91.9 kDa |
| Gene Summary | This gene encodes a member of the EPS8 family. This protein contains one PH domain and one SH3 domain. It functions as part of the EGFR pathway, though its exact role has not been determined. Highly similar proteins in other organisms are involved in the transduction of signals from Ras to Rac and growth factor-mediated actin remodeling. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205300L1 | Lenti ORF clone of Human epidermal growth factor receptor pathway substrate 8 (EPS8), Myc-DDK-tagged |
CNY 11,432.00 |
|
| RC205300L2 | Lenti ORF clone of Human epidermal growth factor receptor pathway substrate 8 (EPS8), mGFP tagged |
CNY 5,890.00 |
|
| RC205300L3 | Lenti ORF clone of Human epidermal growth factor receptor pathway substrate 8 (EPS8), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205300L4 | Lenti ORF clone of Human epidermal growth factor receptor pathway substrate 8 (EPS8), mGFP tagged |
CNY 5,890.00 |
|
| RG205300 | EPS8 (tGFP-tagged) - Human epidermal growth factor receptor pathway substrate 8 (EPS8) |
CNY 10,632.00 |
|
| SC117360 | EPS8 (untagged)-Human epidermal growth factor receptor pathway substrate 8 (EPS8) |
CNY 9,040.00 |
