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C22orf28 (RTCB) (NM_014306) Human Tagged ORF Clone
CAT＃: RC204989
C22orf28 (Myc-DDK-tagged)-Human chromosome 22 open reading frame 28 (C22orf28)
ORF Plasmid: tGFP
"NM_014306" in other vectors (6)
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CNY 3,760.00
CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C22orf28; DJ149A16.6; FAAP; HSPC117 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204989 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTCGCAGCTATAATGATGAGCTGCAGTTCTTGGAGAAGATCAATAAAAACTGCTGGAGGATCAAGA AGGGCTTCGTGCCCAACATGCAGGTTGAAGGTGTTTTCTATGTGAATGATGCTCTGGAGAAATTGATGTT TGAGGAATTAAGGAATGCCTGTCGAGGTGGTGGTGTTGGTGGCTTCCTGCCAGCCATGAAACAGATTGGC AATGTGGCAGCCCTGCCTGGAATTGTTCATCGATCTATTGGGCTTCCTGATGTCCATTCAGGATATGGGT TTGCTATTGGGAACATGGCAGCCTTTGATATGAATGACCCTGAAGCAGTAGTATCCCCAGGTGGTGTCGG GTTTGACATCAACTGTGGTGTCCGCTTGCTAAGAACCAATTTAGATGAAAGTGATGTCCAGCCTGTGAAG GAGCAACTTGCCCAAGCTATGTTTGACCACATTCCTGTTGGGGTGGGGTCAAAAGGTGTCATCCCAATGA ATGCCAAAGACTTGGAGGAGGCCTTGGAGATGGGGGTGGACTGGTCCTTAAGAGAAGGGTATGCCTGGGC TGAAGACAAGGAGCACTGCGAGGAGTACGGAAGGATGCTGCAGGCTGACCCCAATAAAGTTTCTGCAAGG GCGAAGAAAAGAGGCCTTCCTCAGTTGGGGACCCTGGGAGCAGGCAACCATTATGCAGAAATCCAGGTTG TGGATGAGATTTTCAATGAGTATGCTGCTAAAAAAATGGGCATCGACCATAAGGGACAGGTGTGTGTGAT GATCCACAGTGGAAGCAGAGGCTTGGGCCACCAAGTAGCCACAGATGCGCTGGTAGCTATGGAGAAGGCC ATGAAGAGAGACAAGATTATAGTCAATGATCGGCAGTTGGCTTGTGCTCGAATCGCTTCCCCAGAGGGTC AAGACTATCTGAAGGGAATGGCAGCTGCTGGGAACTATGCCTGGGTCAACCGCTCTTCCATGACCTTCTT AACCCGTCAGGCTTTCGCCAAGGTCTTCAACACAACCCCTGATGACTTGGACCTACATGTGATTTATGAT GTTTCTCACAACATTGCCAAAGTGGAGCAGCATGTGGTGGACGGAAAGGAACGGACACTGTTAGTACACA GGAAGGGATCCACCCGCGCTTTCCCTCCTCACCATCCCCTCATTGCTGTTGATTACCAACTCACTGGACA GCCAGTGCTCATTGGTGGCACCATGGGAACCTGTAGTTATGTTCTTACTGGCACTGAACAGGGCATGACT GAGACCTTTGGAACAACCTGTCATGGAGCGGGCCGTGCATTGTCCCGAGCAAAATCTCGACGTAATTTAG ATTTCCAGGATGTCTTAGACAAATTGGCAGATATGGGAATTGCGATCCGTGTTGCCTCACCCAAACTGGT TATGGAAGAGGCTCCTGAGTCCTATAAGAATGTGACAGATGTGGTAAATACCTGCCATGATGCTGGAATC AGCAAGAAAGCCATTAAACTGAGACCAATTGCTGTGATCAAAGGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204989 protein sequence
Red=Cloning site Green=Tags(s) MSRSYNDELQFLEKINKNCWRIKKGFVPNMQVEGVFYVNDALEKLMFEELRNACRGGGVGGFLPAMKQIG NVAALPGIVHRSIGLPDVHSGYGFAIGNMAAFDMNDPEAVVSPGGVGFDINCGVRLLRTNLDESDVQPVK EQLAQAMFDHIPVGVGSKGVIPMNAKDLEEALEMGVDWSLREGYAWAEDKEHCEEYGRMLQADPNKVSAR AKKRGLPQLGTLGAGNHYAEIQVVDEIFNEYAAKKMGIDHKGQVCVMIHSGSRGLGHQVATDALVAMEKA MKRDKIIVNDRQLACARIASPEGQDYLKGMAAAGNYAWVNRSSMTFLTRQAFAKVFNTTPDDLDLHVIYD VSHNIAKVEQHVVDGKERTLLVHRKGSTRAFPPHHPLIAVDYQLTGQPVLIGGTMGTCSYVLTGTEQGMT ETFGTTCHGAGRALSRAKSRRNLDFQDVLDKLADMGIAIRVASPKLVMEEAPESYKNVTDVVNTCHDAGI SKKAIKLRPIAVIKG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014306 |
| ORF Size | 1515 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014306.3 |
| RefSeq Size | 2081 bp |
| RefSeq ORF | 1518 bp |
| Locus ID | 51493 |
| UniProt ID | Q9Y3I0 |
| Domains | UPF0027 |
| MW | 55.2 kDa |
| Gene Summary | Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204989L1 | Lenti ORF clone of Human chromosome 22 open reading frame 28 (C22orf28), Myc-DDK-tagged |
CNY 6,160.00 |
|
| RC204989L2 | Lenti ORF clone of Human chromosome 22 open reading frame 28 (C22orf28), mGFP tagged |
CNY 6,460.00 |
|
| RC204989L3 | Lenti ORF clone of Human chromosome 22 open reading frame 28 (C22orf28), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC204989L4 | Lenti ORF clone of Human chromosome 22 open reading frame 28 (C22orf28), mGFP tagged |
CNY 6,460.00 |
|
| RG204989 | C22orf28 (tGFP-tagged) - Human chromosome 22 open reading frame 28 (C22orf28) |
CNY 5,360.00 |
|
| SC319629 | C22orf28 (untagged)-Human chromosome 22 open reading frame 28 (C22orf28) |
CNY 3,768.00 |
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