United States
Germany
Japan
United Kingdom
China
ZNF289 (ARFGAP2) (NM_032389) Human Tagged ORF Clone
CAT＃: RC204908
ARFGAP2 (Myc-DDK-tagged)-Human ADP-ribosylation factor GTPase activating protein 2 (ARFGAP2)
ORF Plasmid: tGFP
"NM_032389" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 3,880.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | IRZ; NBLA10535; ZFP289; ZNF289 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204908 representing NM_032389
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGAGCCGAACAAGACCGAAATCCAGACTCTTTTTAAGAGGCTTCGCGCAGTTCCAACCAACA AGGCCTGTTTCGACTGCGGCGCCAAGAATCCGAGTTGGGCCAGCATCACGTACGGTGTTTTCTTGTGCAT TGACTGTTCCGGGGTGCACCGCTCCCTGGGCGTCCATCTGAGCTTCATCAGGTCCACAGAGTTGGATTCC AACTGGAACTGGTTCCAGCTGAGGTGTATGCAGGTCGGCGGGAATGCCAATGCGACGGCTTTTTTTCGCC AACATGGATGCACAGCCAATGATGCCAACACCAAATATAATAGCCGAGCTGCCCAGATGTACCGGGAGAA GATCCGGCAGCTGGGGAGTGCGGCCCTGGCTAGGCATGGCACTGATCTTTGGATAGACAACATGAGTAGT GCCGTTCCTAATCACTCCCCAGAGAAGAAGGACTCTGATTTCTTCACAGAACACACTCAACCCCCTGCCT GGGATGCGCCAGCCACTGAGCCTTCAGGGACCCAGCAGCCAGCCCCGTCTACAGAGAGCAGTGGCCTGGC ACAGCCGGAGCATGGCCCCAACACAGACCTGCTTGGCACCTCACCCAAAGCCTCACTGGAACTGAAAAGC TCCATCATTGGCAAGAAGAAGCCAGCAGCAGCTAAGAAAGGGCTGGGTGCCAAGAAAGGCCTAGGGGCCC AGAAGGTGAGCAGCCAGAGCTTCAGTGAGATTGAGCGGCAGGCTCAGGTGGCAGAGAAGCTCCGTGAGCA GCAGGCAGCCGATGCCAAGAAGCAGGCGGAGGAGTCCATGGTCGCCTCCATGCGTCTGGCCTACCAGGAG CTCCAGATTGATCGTAAGAAAGAGGAAAAGAAGCTACAGAATCTGGAAGGGAAGAAGCGAGAGCAGGCAG AAAGGTTGGGCATGGGCTTGGTATCCCGAAGCTCTGTCTCCCACTCCGTGCTGTCTGAGATGCAGGTGAT TGAGCAGGAAACCCCAGTGAGTGCAAAATCCTCTCGCTCGCAGCTGGACTTGTTTGACGATGTTGGTACT TTCGCCTCTGGACCCCCAAAGTACAAGGACAATCCCTTTTCCTTAGGGGAAAGCTTTGGCTCCCGCTGGG ATACAGATGCTGCCTGGGGTATGGACAGGGTAGAGGAGAAGGAGCCAGAAGTGACCATCTCAAGCATCCG GCCTATTTCAGAAAGAGCCACAAACCGGAGGGAAGTGGAGAGCCGGAGCTCAGGCCTCGAGTCTAGTGAG GCGCGTCAGAAATTCGCAGGAGCCAAAGCCATCTCATCTGACATGTTCTTTGGGCGGGAGGTGGATGCGG AGTATGAGGCCAGGTCTCGGCTGCAGCAGCTCTCAGGCAGCAGTGCCATCAGCTCTTCAGACCTCTTTGG GGACATGGATGGAGCTCACGGAGCAGGAAGTGTATCTCTGGGGAACGTGCTGCCTACAGCGGACATTGCC CAGTTTAAGCAGGGTGTCAAGTCTGTGGCTGGGAAAATGGCTGTGCTGGCCAATGGTGTGATGAATTCCT TGCAGGATCGCTACGGTTCCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204908 representing NM_032389
Red=Cloning site Green=Tags(s) MAAEPNKTEIQTLFKRLRAVPTNKACFDCGAKNPSWASITYGVFLCIDCSGVHRSLGVHLSFIRSTELDS NWNWFQLRCMQVGGNANATAFFRQHGCTANDANTKYNSRAAQMYREKIRQLGSAALARHGTDLWIDNMSS AVPNHSPEKKDSDFFTEHTQPPAWDAPATEPSGTQQPAPSTESSGLAQPEHGPNTDLLGTSPKASLELKS SIIGKKKPAAAKKGLGAKKGLGAQKVSSQSFSEIERQAQVAEKLREQQAADAKKQAEESMVASMRLAYQE LQIDRKKEEKKLQNLEGKKREQAERLGMGLVSRSSVSHSVLSEMQVIEQETPVSAKSSRSQLDLFDDVGT FASGPPKYKDNPFSLGESFGSRWDTDAAWGMDRVEEKEPEVTISSIRPISERATNRREVESRSSGLESSE ARQKFAGAKAISSDMFFGREVDAEYEARSRLQQLSGSSAISSSDLFGDMDGAHGAGSVSLGNVLPTADIA QFKQGVKSVAGKMAVLANGVMNSLQDRYGSY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_032389 |
| ORF Size | 1563 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_032389.5 |
| RefSeq Size | 3039 bp |
| RefSeq ORF | 1566 bp |
| Locus ID | 84364 |
| UniProt ID | Q8N6H7 |
| Domains | ArfGap |
| Protein Pathways | Endocytosis |
| MW | 57.2 kDa |
| Gene Summary | GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Implicated in coatomer-mediated protein transport between the Golgi complex and the endoplasmic reticulum. Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
ArfGAP1 acts as a GTPase-activating protein for human ADP-ribosylation factor-like 1 protein
,Feng, HP;Cheng, HY;Hsiao, TF;Lin, TW;Hsu, JW;Huang, LH;Yu, CJ;,
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
,PubMed ID 33715220
[ZNF289]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204908L3 | Lenti-ORF clone of ARFGAP2 (Myc-DDK-tagged)-Human ADP-ribosylation factor GTPase activating protein 2 (ARFGAP2) |
CNY 5,890.00 |
|
| RC204908L4 | Lenti-ORF clone of ARFGAP2 (mGFP-tagged)-Human ADP-ribosylation factor GTPase activating protein 2 (ARFGAP2) |
CNY 5,890.00 |
|
| RG204908 | ARFGAP2 (tGFP-tagged) - Human ADP-ribosylation factor GTPase activating protein 2 (ARFGAP2) |
CNY 4,370.00 |
|
| SC104758 | ARFGAP2 (untagged)-Human ADP-ribosylation factor GTPase activating protein 2 (ARFGAP2) |
CNY 7,220.00 |
