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NPR2L (NPRL2) (NM_006545) Human Tagged ORF Clone
CAT＃: RC204818
NPRL2 (Myc-DDK-tagged)-Human nitrogen permease regulator-like 2 (S. cerevisiae) (NPRL2)
ORF Plasmid: tGFP
"NM_006545" in other vectors (5)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | FFEVF2; NPR2; NPR2L; TUSC4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204818 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAGCGGCTGCCGCATCGAATGCATATTCTTCAGCGAGTTCCACCTCACGCTGGGACCCAAGATCA CCTATCAGGTCCCTGAAGACTTCATCTCCCGAGAGCTGTTTGACACAGTCCAAGTGTACATCATCACCAA GCCAGAGCTGCAGAACAAGCTTATCACTGTCACAGCTATGGAAAAGAAGCTGATCGGCTGTCCTGTGTGC ATCGAACACAAGAAGTACAGCCGCAATGCTCTCCTCTTCAACCTGGGCTTCGTGTGTGATGCCCAGGCCA AGACCTGCGCCCTCGAGCCCATTGTTAAAAAGCTGGCTGGCTATCTGACCACACTAGAGCTAGAGAGCAG CTTCGTGTCCATGGAGGAGAGCAAGCAGAAGTTGGTGCCCATCATGACCATCTTGCTGGAGGAGCTAAAT GCCTCAGGCCGGTGCACTCTGCCCATTGATGAGTCCAACACCATCCACTTGAAGGTGATTGAGCAGCGGC CAGACCCTCCGGTGGCCCAGGAGTATGATGTACCTGTCTTTACCAAAGACAAGGAGGATTTCTTCAACTC ACAGTGGGACCTCACTACACAACAAATCCTGCCCTACATTGATGGGTTCCGCCACATCCAGAAGATTTCA GCAGAGGCAGATGTGGAGCTCAACCTGGTGCGCATTGCTATCCAGAACCTGCTGTACTACGGCGTTGTGA CACTGGTGTCCATCCTCCAGTACTCCAATGTATACTGCCCAACGCCCAAGGTCCAGGACCTGGTAGATGA CAAGTCCCTGCAAGAGGCATGTCTATCCTACGTGACCAAGCAAGGGCACAAGAGGGCCAGTCTCCGGGAT GTGTTCCAGCTATACTGCAGCCTGAGCCCTGGCACTACCGTGCGAGACCTCATTGGCCGCCACCCCCAGC AGCTGCAGCATGTTGATGAACGGAAGCTGATCCAGTTCGGGCTTATGAAGAACCTCATCAGGCGACTACA GAAGTATCCTGTGCGGGTGACTCGGGAAGAGCAGAGCCACCCTGCCCGGCTTTATACAGGCTGCCACAGC TATGACGAGATCTGCTGCAAGACAGGCATGAGCTACCATGAGCTGGATGAGCGGCTTGAAAATGACCCCA ACATCATCATCTGCTGGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204818 protein sequence
Red=Cloning site Green=Tags(s) MGSGCRIECIFFSEFHLTLGPKITYQVPEDFISRELFDTVQVYIITKPELQNKLITVTAMEKKLIGCPVC IEHKKYSRNALLFNLGFVCDAQAKTCALEPIVKKLAGYLTTLELESSFVSMEESKQKLVPIMTILLEELN ASGRCTLPIDESNTIHLKVIEQRPDPPVAQEYDVPVFTKDKEDFFNSQWDLTTQQILPYIDGFRHIQKIS AEADVELNLVRIAIQNLLYYGVVTLVSILQYSNVYCPTPKVQDLVDDKSLQEACLSYVTKQGHKRASLRD VFQLYCSLSPGTTVRDLIGRHPQQLQHVDERKLIQFGLMKNLIRRLQKYPVRVTREEQSHPARLYTGCHS YDEICCKTGMSYHELDERLENDPNIIICWK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006545 |
| ORF Size | 1140 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006545.3 |
| RefSeq Size | 1672 bp |
| RefSeq ORF | 1143 bp |
| Locus ID | 10641 |
| UniProt ID | Q8WTW4 |
| Protein Families | Druggable Genome |
| MW | 43.7 kDa |
| Gene Summary | As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204818L3 | Lenti ORF clone of Human nitrogen permease regulator-like 2 (S. cerevisiae) (NPRL2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204818L4 | Lenti ORF clone of Human nitrogen permease regulator-like 2 (S. cerevisiae) (NPRL2), mGFP tagged |
CNY 5,890.00 |
|
| RG204818 | NPRL2 (tGFP-tagged) - Human nitrogen permease regulator-like 2 (S. cerevisiae) (NPRL2) |
CNY 5,256.00 |
|
| SC116029 | NPRL2 (untagged)-Human nitrogen permease regulator-like 2 (S. cerevisiae) (NPRL2) |
CNY 3,656.00 |
|
| SC321098 | NPRL2 (untagged)-Human nitrogen permease regulator-like 2 (S. cerevisiae) (NPRL2) |
CNY 3,656.00 |
