FAM172A (NM_032042) Human Tagged ORF Clone

CAT#: RC204600

FAM172A (Myc-DDK-tagged)-Human family with sequence similarity 172, member A (FAM172A), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_032042" in other vectors (5)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-FAM172A Antibody
    • 100 ul

CNY 5,250.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms C5orf21; Toupee
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC204600 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTATTTCCTTGAGCTCTTTAATTTTGTTGCCAATTTGGATAAACATGGCACAAATCCAGCAGGGAG
GTCCAGATGAAAAAGAAAAGACTACCGCACTGAAAGATTTATTATCTAGGATAGATTTGGATGAACTAAT
GAAAAAAGATGAACCGCCTCTTGATTTTCCTGATACCCTGGAAGGATTTGAATATGCTTTTAATGAAAAG
GGACAGTTAAGACACATAAAAACTGGGGAACCATTTGTTTTTAACTACCGGGAAGATTTACACAGATGGA
ACCAGAAAAGATACGAGGCTCTAGGAGAGATCATCACGAAGTATGTATATGAGCTCCTGGAAAAGGATTG
TAATTTGAAAAAAGTATCTATTCCAGTAGATGCCACTGAGAGTGAACCAAAGAGTTTTATCTTTATGAGT
GAGGATGCTTTGACAAATCCACAGAAACTGATGGTTTTAATTCATGGTAGTGGTGTTGTCAGGGCAGGGC
AGTGGGCTAGAAGACTTATTATAAATGAAGATCTGGACAGTGGCACACAGATACCGTTTATTAAAAGAGC
TGTGGCTGAAGGATATGGAGTAATAGTACTAAATCCCAATGAAAACTATATTGAAGTAGAAAAGCCGAAG
ATACACGTACAGTCATCATCTGATAGTTCAGATGAACCAGCAGAAAAACGGGAAAGAAAAGATAAAGTTT
CTAAAGAAACAAAGAAGCGACGTGATTTCTATGAGAAGTATCGTAACCCCCAAAGAGAAAAAGAAATGAT
GCAATTGTATATCAGAGAAAATGGTTCTCCTGAAGAACATGCAATCTATGTTTGGGATCATTTCATAGCT
CAGGCTGCTGCTGAGAATGTGTTTTTCGTTGCTCACAGCTATGGAGGACTTGCTTTTGTTGAACTGATGA
TTCAACGAGAAGCCGATGTAAAAAATAAGGTAACTGCTGTGGCATTGACAGACTCTGTTCACAATGTGTG
GCATCAAGAAGCTGGCAAAACGATTCGAGAATGGATGAGAGAGAACTGTTGTAATTGGGTCTCTAGCTCA
GAACCATTAGACACATCAGTGGAGTCCATGCTACCTGATTGCCCCCGGGTCTCAGCAGGCACCGACCGTC
ACGAGCTAACTTCCTGGAAGAGCTTTCCGTCTATTTTCAAATTCTTTACCGAAGCCTCAGAGGCCAAGAC
CAGCTCCCTGAAGCCGGCTGTGACGCGCCGCTCCCACCGCATCAAGCACGAAGAGCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC204600 protein sequence
Red=Cloning site Green=Tags(s)

MSISLSSLILLPIWINMAQIQQGGPDEKEKTTALKDLLSRIDLDELMKKDEPPLDFPDTLEGFEYAFNEK
GQLRHIKTGEPFVFNYREDLHRWNQKRYEALGEIITKYVYELLEKDCNLKKVSIPVDATESEPKSFIFMS
EDALTNPQKLMVLIHGSGVVRAGQWARRLIINEDLDSGTQIPFIKRAVAEGYGVIVLNPNENYIEVEKPK
IHVQSSSDSSDEPAEKRERKDKVSKETKKRRDFYEKYRNPQREKEMMQLYIRENGSPEEHAIYVWDHFIA
QAAAENVFFVAHSYGGLAFVELMIQREADVKNKVTAVALTDSVHNVWHQEAGKTIREWMRENCCNWVSSS
EPLDTSVESMLPDCPRVSAGTDRHELTSWKSFPSIFKFFTEASEAKTSSLKPAVTRRSHRIKHEEL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_032042
ORF Size 1248 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_032042.6
RefSeq Size 4720 bp
RefSeq ORF 1251 bp
Locus ID 83989
UniProt ID Q8WUF8
MW 48 kDa
Gene Summary Plays a role in the regulation of alternative splicing, by interacting with AGO2 and CHD7. Seems to be required for stabilizing protein-protein interactions at the chromatin-spliceosome interface. May have hydrolase activity.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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