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Rad9 (RAD9A) (NM_004584) Human Tagged ORF Clone
CAT＃: RC204439
RAD9A (Myc-DDK-tagged)-Human RAD9 homolog A (S. pombe) (RAD9A)
ORF Plasmid: tGFP
"NM_004584" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | RAD9 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204439 representing NM_004584.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAAGTGCCTGGTCACGGGCGGCAACGTGAAGGTGCTCGGCAAGGCCGTCCACTCCCTGTCCCGCATC GGGGACGAGCTCTACCTGGAACCCTTGGAGGACGGGCTCTCCCTCCGGACGGTGAACTCCTCCCGCTCT GCCTATGCCTGCTTTCTCTTTGCCCCGCTCTTCTTCCAGCAATACCAGGCAGCCACCCCTGGTCAGGAC CTGCTGCGCTGTAAGATCCTGATGAAGTCTTTCCTGTCTGTCTTCCGCTCACTGGCGATGCTGGAGAAG ACGGTGGAAAAATGCTGCATCTCCCTGAATGGCCGGAGCAGCCGCCTGGTGGTCCAGCTGCATTGCAAG TTCGGGGTGCGGAAGACTCACAACCTGTCCTTCCAGGACTGTGAGTCCCTGCAGGCCGTCTTCGACCCA GCCTCGTGCCCCCACATGCTCCGCGCCCCAGCACGGGTTCTGGGGGAGGCTGTTCTGCCCTTCTCTCCT GCACTGGCTGAAGTGACGCTGGGCATTGGCCGTGGCCGCAGGGTCATCCTGCGCAGCTACCACGAGGAG GAGGCAGACAGCACTGCCAAAGCCATGGTGACTGAGATGTGCCTTGGAGAGGAGGATTTCCAGCAGCTG CAGGCCCAGGAAGGGGTGGCCATCACTTTCTGCCTCAAGGAATTCCGGGGGCTCCTGAGCTTTGCAGAG TCAGCAAACTTGAATCTTAGCATTCATTTTGATGCTCCAGGCAGGCCCGCCATCTTCACCATCAAGGAC TCTTTGCTGGACGGCCACTTTGTCTTGGCCACACTCTCAGACACCGACTCGCACTCCCAGGACCTGGGC TCCCCAGAGCGTCACCAGCCAGTGCCTCAGCTCCAGGCTCACAGCACACCCCACCCGGACGACTTTGCC AATGACGACATTGACTCTTACATGATCGCCATGGAAACCACTATAGGCAATGAGGGCTCGCGGGTGCTG CCCTCCATTTCCCTTTCACCTGGCCCCCAGCCCCCCAAGAGCCCCGGTCCCCACTCCGAGGAGGAAGAT GAGGCTGAGCCCAGTACAGTGCCTGGGACTCCCCCACCCAAGAAGTTCCGCTCACTGTTCTTCGGCTCC ATCCTGGCCCCTGTACGCTCCCCCCAGGGCCCCAGCCCTGTGCTGGCGGAAGACAGTGAGGGTGAAGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC >Peptide sequence encoded by RC204439
Blue=ORF Red=Cloning site Green=Tag(s) MKCLVTGGNVKVLGKAVHSLSRIGDELYLEPLEDGLSLRTVNSSRSAYACFLFAPLFFQQYQAATPGQD LLRCKILMKSFLSVFRSLAMLEKTVEKCCISLNGRSSRLVVQLHCKFGVRKTHNLSFQDCESLQAVFDP ASCPHMLRAPARVLGEAVLPFSPALAEVTLGIGRGRRVILRSYHEEEADSTAKAMVTEMCLGEEDFQQL QAQEGVAITFCLKEFRGLLSFAESANLNLSIHFDAPGRPAIFTIKDSLLDGHFVLATLSDTDSHSQDLG SPERHQPVPQLQAHSTPHPDDFANDDIDSYMIAMETTIGNEGSRVLPSISLSPGPQPPKSPGPHSEEED EAEPSTVPGTPPPKKFRSLFFGSILAPVRSPQGPSPVLAEDSEGEG TRTRPLEQKLISEEDLAANDILDYKDDDDKV Recombinant protein using RC204439 also available, TP304439 |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004584 |
| ORF Size | 1173 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004584.3 |
| RefSeq Size | 2128 bp |
| RefSeq ORF | 1176 bp |
| Locus ID | 5883 |
| UniProt ID | Q99638 |
| Domains | Rad9 |
| Protein Families | Druggable Genome, Stem cell - Pluripotency |
| MW | 42.5 kDa |
| Gene Summary | This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair. This protein possesses 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The CDK-PLK1 axis targets the DNA damage checkpoint sensor protein RAD9 to promote cell proliferation and tolerance to genotoxic stress
,Wakida, T;Ikura, M;Kuriya, K;Ito, S;Shiroiwa, Y;Habu, T;Kawamoto, T;Okumura, K;Ikura, T;Furuya, K;,
Elife
,PubMed ID 29254517
[Rad9]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204439L1 | Lenti ORF clone of Human RAD9 homolog A (S. pombe) (RAD9A), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC204439L2 | Lenti ORF clone of Human RAD9 homolog A (S. pombe) (RAD9A), mGFP tagged |
CNY 5,890.00 |
|
| RC204439L3 | Lenti ORF clone of Human RAD9 homolog A (S. pombe) (RAD9A), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204439L4 | Lenti ORF clone of Human RAD9 homolog A (S. pombe) (RAD9A), mGFP tagged |
CNY 5,890.00 |
|
| RG204439 | RAD9A (tGFP-tagged) - Human RAD9 homolog A (S. pombe) (RAD9A) |
CNY 5,256.00 |
|
| SC321679 | RAD9A (untagged)-Human RAD9 homolog A (S. pombe) (RAD9A) |
CNY 3,656.00 |
