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TYK2 (NM_003331) Human Tagged ORF Clone
CAT＃: RC204351
- TrueORF®
TYK2 (Myc-DDK-tagged)-Human tyrosine kinase 2 (TYK2)
ORF Plasmid: tGFP
"NM_003331" in other vectors (7)
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CNY 12,400.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | IMD35; JTK1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204351 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCTGCGCCACTGGGGGATGGCCAGGGGCAGTAAGCCCGTTGGGGATGGAGCCCAGCCCATGGCTG CCATGGGAGGCCTGAAGGTGCTTCTGCACTGGGCTGGTCCAGGCGGCGGGGAGCCCTGGGTCACTTTCAG TGAGTCATCGCTGACAGCTGAGGAAGTCTGCATCCACATTGCACATAAAGTTGGTATCACTCCTCCTTGC TTCAATCTCTTTGCCCTCTTCGATGCTCAGGCCCAAGTCTGGTTGCCCCCAAACCACATCCTAGAGATCC CCAGAGATGCAAGCCTGATGCTATATTTCCGCATAAGGTTTTATTTCCGGAACTGGCATGGCATGAATCC TCGGGAACCGGCTGTGTACCGTTGTGGGCCCCCAGGAACCGAGGCATCCTCAGATCAGACAGCACAGGGG ATGCAACTCCTGGACCCAGCCTCATTTGAGTACCTCTTTGAGCAGGGCAAGCATGAGTTTGTGAATGACG TGGCATCACTGTGGGAGCTGTCGACCGAGGAGGAGATCCACCACTTTAAGAATGAGAGCCTGGGCATGGC CTTTCTGCACCTCTGTCACCTCGCTCTCCGCCATGGCATCCCCCTGGAGGAGGTGGCCAAGAAGACCAGC TTCAAGGACTGCATCCCGCGCTCCTTCCGCCGGCATATCCGGCAGCACAGCGCCCTGACCCGGCTGCGCC TTCGGAACGTCTTCCGCAGGTTCCTGCGGGACTTCCAGCCGGGCCGACTCTCCCAGCAGATGGTCATGGT CAAATACCTAGCCACACTCGAGCGGCTGGCACCCCGCTTCGGCACAGAGCGTGTGCCCGTGTGCCACCTG AGGCTGCTGGCCCAGGCCGAGGGGGAGCCCTGCTACATCCGGGACAGTGGGGTGGCCCCTACAGACCCTG GCCCTGAGTCTGCTGCTGGGCCCCCAACCCACGAGGTGCTGGTGACAGGCACTGGTGGCATCCAGTGGTG GCCAGTAGAGGAGGAGGTGAACAAGGAGGAGGGTTCTAGTGGCAGCAGTGGCAGGAACCCCCAAGCCAGC CTGTTTGGGAAGAAGGCCAAGGCTCACAAGGCAGTCGGCCAGCCGGCAGACAGGCCGCGGGAGCCACTGT GGGCCTACTTCTGTGACTTCCGGGACATCACCCACGTGGTGCTGAAAGAGCACTGTGTCAGCATCCACCG GCAGGACAACAAGTGCCTGGAGCTGAGCTTGCCTTCCCGGGCTGCGGCGCTGTCCTTCGTGTCGCTGGTG GACGGCTATTTCCGCCTGACGGCCGACTCCAGCCACTACCTGTGCCACGAGGTGGCTCCCCCACGGCTGG TGATGAGCATCCGGGATGGGATCCACGGACCCCTGCTGGAGCCATTTGTGCAGGCCAAGCTGCGGCCCGA GGACGGCCTGTACCTCATTCACTGGAGCACCAGCCACCCCTACCGCCTGATCCTCACAGTGGCCCAGCGT AGCCAGGCACCAGACGGCATGCAGAGCTTGCGGCTCCGAAAGTTCCCCATTGAGCAGCAGGACGGGGCCT TCGTGCTGGAGGGCTGGGGCCGGTCCTTCCCCAGCGTTCGGGAACTTGGGGCTGCCTTGCAGGGCTGCTT GCTGAGGGCCGGGGATGACTGCTTCTCTCTGCGTCGCTGTTGCCTGCCCCAACCAGGAGAAACCTCCAAT CTCATCATCATGCGGGGGGCTCGGGCCAGCCCCAGGACACTCAACCTCAGCCAGCTCAGCTTCCACCGGG TTGACCAGAAGGAGATCACCCAGCTGTCCCACTTGGGCCAGGGCACAAGGACCAACGTGTATGAGGGCCG CCTGCGAGTGGAGGGCAGCGGGGACCCTGAGGAGGGCAAGATGGATGACGAGGACCCCCTCGTGCCTGGC AGGGACCGTGGGCAGGAGCTACGAGTGGTGCTCAAAGTGCTGGACCCTAGTCACCATGACATCGCCCTGG CCTTCTACGAGACAGCCAGCCTCATGAGCCAGGTCTCCCACACGCACCTGGCCTTCGTGCATGGCGTCTG TGTGCGCGGCCCTGAAAATATCATGGTGACAGAGTACGTGGAGCACGGACCCCTGGATGTGTGGCTGCGG AGGGAGCGGGGCCATGTGCCCATGGCTTGGAAGATGGTGGTGGCCCAGCAGCTGGCCAGCGCCCTCAGCT ACCTGGAGAACAAGAACCTGGTTCATGGTAATGTGTGTGGCCGGAACATCCTGCTGGCCCGGCTGGGGTT GGCAGAGGGCACCAGCCCCTTCATCAAGCTGAGTGATCCTGGCGTGGGCCTGGGCGCCCTCTCCAGGGAG GAGCGGGTGGAGAGGATCCCCTGGCTGGCCCCCGAATGCCTACCAGGTGGGGCCAACAGCCTAAGCACCG CCATGGACAAGTGGGGGTTTGGCGCCACCCTCCTGGAGATCTGCTTTGACGGAGAGGCCCCTCTGCAGAG CCGCAGTCCCTCCGAGAAGGAGCATTTCTACCAGAGGCAGCACCGGCTGCCCGAGCCCTCCTGCCCACAG CTGGCCACACTCACCAGCCAGTGTCTGACCTATGAGCCAACCCAGAGGCCATCATTCCGCACCATCCTGC GTGACCTCACCCGGCTGCAGCCCCACAATCTTGCTGACGTCTTGACTGTGAACCCGGACTCACCGGCGTC GGACCCTACGGTTTTCCACAAGCGCTATTTGAAAAAGATCCGAGATCTGGGCGAGGGTCACTTCGGCAAG GTCAGCTTGTACTGCTACGATCCGACCAACGACGGCACTGGCGAGATGGTGGCGGTGAAAGCCCTCAAGG CAGACTGCGGCCCCCAGCACCGCTCGGGCTGGAAGCAGGAGATTGACATTCTGCGCACGCTCTACCACGA GCACATCATCAAGTACAAGGGCTGCTGCGAGGACCAAGGCGAGAAGTCGCTGCAGCTGGTCATGGAGTAC GTGCCCCTGGGCAGCCTCCGAGACTACCTGCCCCGGCACAGCATCGGGCTGGCCCAGCTGCTGCTCTTCG CCCAGCAGATCTGCGAGGGCATGGCCTATCTGCACTCGCAGCACTACATCCACCGAGACCTAGCCGCGCG CAACGTGCTGCTGGACAACGACAGGCTGGTCAAGATCGGGGACTTTGGCCTAGCCAAGGCCGTGCCCGAA GGCCACGAGTACTACCGCGTGCGCGAGGATGGGGACAGCCCCGTGTTCTGGTATGCCCCAGAGTGCCTGA AGGAGTATAAGTTCTACTATGCGTCAGATGTCTGGTCCTTCGGGGTGACCCTGTATGAGCTGCTGACGCA CTGTGACTCCAGCCAGAGCCCCCCCACGAAATTCCTTGAGCTCATAGGCATTGCTCAGGGTCAGATGACA GTTCTGAGACTCACTGAGTTGCTGGAACGAGGGGAGAGGCTGCCACGGCCCGACAAATGTCCCTGTGAGG TCTATCATCTCATGAAGAACTGCTGGGAGACAGAGGCGTCCTTTCGCCCAACCTTCGAGAACCTCATACC CATTCTGAAGACAGTCCATGAGAAGTACCAAGGCCAGGCCCCTTCAGTGTTCAGCGTGTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204351 protein sequence
Red=Cloning site Green=Tags(s) MPLRHWGMARGSKPVGDGAQPMAAMGGLKVLLHWAGPGGGEPWVTFSESSLTAEEVCIHIAHKVGITPPC FNLFALFDAQAQVWLPPNHILEIPRDASLMLYFRIRFYFRNWHGMNPREPAVYRCGPPGTEASSDQTAQG MQLLDPASFEYLFEQGKHEFVNDVASLWELSTEEEIHHFKNESLGMAFLHLCHLALRHGIPLEEVAKKTS FKDCIPRSFRRHIRQHSALTRLRLRNVFRRFLRDFQPGRLSQQMVMVKYLATLERLAPRFGTERVPVCHL RLLAQAEGEPCYIRDSGVAPTDPGPESAAGPPTHEVLVTGTGGIQWWPVEEEVNKEEGSSGSSGRNPQAS LFGKKAKAHKAVGQPADRPREPLWAYFCDFRDITHVVLKEHCVSIHRQDNKCLELSLPSRAAALSFVSLV DGYFRLTADSSHYLCHEVAPPRLVMSIRDGIHGPLLEPFVQAKLRPEDGLYLIHWSTSHPYRLILTVAQR SQAPDGMQSLRLRKFPIEQQDGAFVLEGWGRSFPSVRELGAALQGCLLRAGDDCFSLRRCCLPQPGETSN LIIMRGARASPRTLNLSQLSFHRVDQKEITQLSHLGQGTRTNVYEGRLRVEGSGDPEEGKMDDEDPLVPG RDRGQELRVVLKVLDPSHHDIALAFYETASLMSQVSHTHLAFVHGVCVRGPENIMVTEYVEHGPLDVWLR RERGHVPMAWKMVVAQQLASALSYLENKNLVHGNVCGRNILLARLGLAEGTSPFIKLSDPGVGLGALSRE ERVERIPWLAPECLPGGANSLSTAMDKWGFGATLLEICFDGEAPLQSRSPSEKEHFYQRQHRLPEPSCPQ LATLTSQCLTYEPTQRPSFRTILRDLTRLQPHNLADVLTVNPDSPASDPTVFHKRYLKKIRDLGEGHFGK VSLYCYDPTNDGTGEMVAVKALKADCGPQHRSGWKQEIDILRTLYHEHIIKYKGCCEDQGEKSLQLVMEY VPLGSLRDYLPRHSIGLAQLLLFAQQICEGMAYLHSQHYIHRDLAARNVLLDNDRLVKIGDFGLAKAVPE GHEYYRVREDGDSPVFWYAPECLKEYKFYYASDVWSFGVTLYELLTHCDSSQSPPTKFLELIGIAQGQMT VLRLTELLERGERLPRPDKCPCEVYHLMKNCWETEASFRPTFENLIPILKTVHEKYQGQAPSVFSVC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003331 |
| ORF Size | 3561 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003331.3, NP_003322.2 |
| RefSeq Size | 4262 bp |
| RefSeq ORF | 3564 bp |
| Locus ID | 7297 |
| UniProt ID | P29597 |
| Domains | B41, pkinase, SH2, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Jak-STAT signaling pathway |
| MW | 133.7 kDa |
| Gene Summary | This gene encodes a member of the tyrosine kinase and, more specifically, the Janus kinases (JAKs) protein families. This protein associates with the cytoplasmic domain of type I and type II cytokine receptors and promulgate cytokine signals by phosphorylating receptor subunits. It is also a component of both the type I and type III interferon signaling pathways. As such, it may play a role in anti-viral immunity. A mutation in this gene has been associated with Immunodeficiency 35. [provided by RefSeq, Sep 2020] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Selective inhibitors of JAK1 targeting an isoform-restricted allosteric cysteine.
,null,
Nature chemical biology
,PubMed ID 36097295
[TYK2]
|
|
HIV blocks Type I IFN signaling through disruption of STAT1 phosphorylation
,Nguyen, NV;Tran, JT;Sanchez, DJ;,
Innate Immun
,PubMed ID 30282499
[TYK2]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204351L1 | Lenti ORF clone of Human tyrosine kinase 2 (TYK2), Myc-DDK-tagged |
CNY 14,800.00 |
|
| RC204351L2 | Lenti ORF clone of Human tyrosine kinase 2 (TYK2), mGFP tagged |
CNY 12,070.00 |
|
| RC204351L3 | Lenti ORF clone of Human tyrosine kinase 2 (TYK2), Myc-DDK-tagged |
CNY 12,070.00 |
|
| RC204351L4 | Lenti ORF clone of Human tyrosine kinase 2 (TYK2), mGFP tagged |
CNY 14,800.00 |
|
| RG204351 | TYK2 (tGFP-tagged) - Human tyrosine kinase 2 (TYK2) |
CNY 14,000.00 |
|
| SC128128 | TYK2 (untagged)-Human tyrosine kinase 2 (TYK2) |
CNY 8,272.00 |
|
| SC323566 | TYK2 (untagged)-Kinase deficient mutant (K930M) of Human tyrosine kinase 2 (TYK2) |
CNY 8,272.00 |
