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Hephaestin (HEPH) (NM_014799) Human Tagged ORF Clone
CAT＃: RC204175
HEPH (Myc-DDK-tagged)-Human hephaestin (HEPH), transcript variant 2
ORF Plasmid: tGFP
"NM_014799" in other vectors (4)
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CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CPL |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204175 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCAGGCCACCTCCTCTGGGCTCTGCTGTTCATGCAGTCCTTGTGGCCTCAACTGACTGATGGAG CCACTCGAGTCTACTACCTGGGCATCCGGGATGTGCAGTGGAACTATGCTCCCAAGGGAAGAAATGTCAT CACGAACCAGCCTCTGGACAGTGACATAGTGGCTTCCAGCTTCTTAAAGTCTGACAAGAACCGGATAGGG GGAACCTACAAGAAGACCATCTATAAAGAATACAAGGATGACTCATACACAGATGAAGTGGCCCAGCCTG CCTGGTTGGGCTTCCTGGGGCCAGTGTTGCAGGCTGAAGTGGGGGATGTCATTCTTATTCACCTGAAGAA TTTTGCCACTCGTCCCTATACCATCCACCCTCATGGTGTCTTCTACGAGAAGGACTCTGAAGGTTCCCTA TACCCAGATGGCTCCTCTGGGCCACTGAAAGCTGATGACTCTGTTCCCCCGGGGGGCAGCCATATCTACA ACTGGACCATTCCAGAAGGCCATGCACCCACCGATGCTGACCCAGCGTGCCTCACCTGGATCTACCATTC TCATGTAGATGCTCCACGAGACATTGCAACTGGCCTAATTGGGCCTCTCATCACCTGTAAAAGAGGAGCC CTGGATGGGAACTCCCCTCCTCAACGCCAGGATGTAGACCATGATTTCTTCCTCCTCTTCAGTGTGGTAG ATGAGAACCTCAGCTGGCATCTCAATGAGAACATTGCCACTTACTGCTCAGATCCTGCTTCAGTGGACAA AGAAGATGAGACATTTCAGGAGAGCAATAGGATGCATGCAATCAATGGCTTTGTTTTTGGGAATTTACCT GAGCTGAACATGTGTGCACAGAAACGTGTGGCCTGGCACTTGTTTGGCATGGGCAATGAAATTGATGTCC ACACAGCATTTTTCCATGGACAGATGCTGACTACCCGTGGACACCACACTGATGTGGCTAACATCTTTCC AGCCACCTTTGTGACTGCTGAGATGGTGCCCTGGGAACCTGGTACCTGGTTAATTAGCTGCCAAGTGAAC AGTCACTTTCGAGATGGCATGCAGGCACTCTACAAGGTCAAGTCTTGCTCCATGGCCCCTCCTGTGGACC TGCTCACAGGCAAAGTTCGACAGTACTTCATTGAGGCCCATGAGATTCAATGGGACTATGGCCCGATGGG GCATGATGGGAGTACTGGGAAGAATTTGAGAGAGCCAGGCAGTATCTCAGATAAGTTTTTCCAGAAGAGC TCCAGCCGAATTGGGGGCACTTACTGGAAAGTGCGATATGAAGCCTTTCAAGATGAGACATTCCAAGAGA AGATGCATTTGGAGGAAGATAGGCATCTTGGAATCCTGGGGCCAGTGATCCGGGCTGAGGTGGGTGACAC CATTCAGGTGGTCTTCTACAACCGTGCCTCCCAGCCATTCAGCATGCAGCCCCATGGGGTCTTTTATGAG AAAGACTATGAAGGCACTGTGTACAATGATGGCTCATCTTACCCTGGCTTGGTTGCCAAGCCCTTTGAGA AAGTAACATACCGCTGGACAGTCCCCCCTCATGCCGGTCCCACTGCTCAGGATCCTGCTTGTCTCACTTG GATGTACTTCTCTGCTGCAGATCCCATAAGAGACACAAATTCTGGCCTGGTGGGCCCGCTGCTGGTGTGC AGGGCTGGTGCCTTGGGTGCAGATGGCAAGCAGAAAGGGGTGGATAAAGAATTCTTTCTTCTCTTCACTG TGTTGGATGAGAACAAGAGCTGGTACAGCAATGCCAATCAAGCAGCTGCTATGTTGGATTTCCGACTGCT TTCAGAGGATATTGAGGGCTTCCAAGACTCCAATCGGATGCATGCCATTAATGGGTTTCTGTTCTCTAAC CTGCCCAGGCTGGACATGTGCAAGGGTGACACAGTGGCCTGGCACCTGCTCGGCCTGGGCACAGAGACTG ATGTGCATGGAGTCATGTTCCAGGGCAACACTGTGCAGCTTCAGGGCATGAGGAAGGGTGCAGCTATGCT CTTTCCTCATACCTTTGTCATGGCCATCATGCAGCCTGACAACCTTGGGACATTTGAGATTTATTGCCAG GCAGGCAGCCATCGAGAAGCAGGGATGAGGGCAATCTATAATGTCTCCCAGTGTCCTGGCCACCAAGCCA CCCCTCGCCAACGCTACCAAGCTGCAAGAATCTACTATATCATGGCAGAAGAAGTAGAGTGGGACTATTG CCCTGACCGGAGCTGGGAACGGGAATGGCACAACCAGTCTGAGAAGGACAGTTATGGTTACATTTTCCTG AGCAACAAGGATGGGCTCCTGGGTTCCAGATACAAGAAAGCTGTATTCAGGGAATACACTGATGGTACAT TCAGGATCCCTCGGCCAAGGACTGGACCAGAAGAACACTTGGGAATCTTGGGTCCACTTATCAAAGGTGA AGTTGGTGATATCCTGACTGTGGTATTCAAGAATAATGCCAGCCGCCCCTACTCTGTGCATGCTCATGGA GTGCTAGAATCTACTACTGTCTGGCCACTGGCTGCTGAGCCTGGTGAGGTGGTCACTTATCAGTGGAACA TCCCAGAGAGGTCTGGCCCTGGGCCCAATGACTCTGCTTGTGTTTCCTGGATCTATTATTCTGCAGTGGA TCCCATCAAGGACATGTATAGTGGCCTGGTGGGGCCCTTGGCTATCTGCCAAAAGGGCATCCTGGAGCCC CATGGAGGACGGAGTGACATGGATCGGGAATTTGCATTGTTGTTCTTGATTTTTGATGAAAATAAGTCTT GGTATTTGGAGGAAAATGTGGCAACCCATGGGTCCCAGGATCCAGGCAGTATTAACCTACAGGATGAAAC TTTCTTGGAGAGCAATAAAATGCATGCAATCAATGGGAAACTCTATGCCAACCTTAGGGGTCTTACCATG TACCAAGGAGAACGAGTGGCCTGGTACATGCTGGCCATGGGCCAAGATGTGGATCTACACACCATCCACT TTCATGCAGAGAGCTTCCTCTATCGGAATGGCGAGAACTACCGGGCAGATGTGGTGGATCTGTTCCCAGG GACTTTTGAGGTTGTGGAGATGGTGGCCAGCAACCCTGGGACATGGCTGATGCACTGCCATGTGACTGAC CATGTCCATGCTGGCATGGAGACCCTCTTCACTGTTTTTTCTCGAACAGAACACTTAAGCCCTCTCACCG TCATCACCAAAGAGACTGAAAAAGCAGTGCCCCCCAGAGACATTGAAGAAGGCAATGTGAAGATGCTGGG CATGCAGATCCCCATAAAGAATGTTGAGATGCTGGCCTCTGTTTTGGTTGCCATTAGTGTCACCCTTCTG CTCGTTGTTCTGGCTCTTGGTGGAGTGGTTTGGTACCAACATCGACAGAGAAAGCTACGACGCAATAGGA GGTCCATCCTGGATGACAGCTTCAAGCTTCTGTCTTTCAAACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204175 protein sequence
Red=Cloning site Green=Tags(s) MESGHLLWALLFMQSLWPQLTDGATRVYYLGIRDVQWNYAPKGRNVITNQPLDSDIVASSFLKSDKNRIG GTYKKTIYKEYKDDSYTDEVAQPAWLGFLGPVLQAEVGDVILIHLKNFATRPYTIHPHGVFYEKDSEGSL YPDGSSGPLKADDSVPPGGSHIYNWTIPEGHAPTDADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGA LDGNSPPQRQDVDHDFFLLFSVVDENLSWHLNENIATYCSDPASVDKEDETFQESNRMHAINGFVFGNLP ELNMCAQKRVAWHLFGMGNEIDVHTAFFHGQMLTTRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVN SHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKS SSRIGGTYWKVRYEAFQDETFQEKMHLEEDRHLGILGPVIRAEVGDTIQVVFYNRASQPFSMQPHGVFYE KDYEGTVYNDGSSYPGLVAKPFEKVTYRWTVPPHAGPTAQDPACLTWMYFSAADPIRDTNSGLVGPLLVC RAGALGADGKQKGVDKEFFLLFTVLDENKSWYSNANQAAAMLDFRLLSEDIEGFQDSNRMHAINGFLFSN LPRLDMCKGDTVAWHLLGLGTETDVHGVMFQGNTVQLQGMRKGAAMLFPHTFVMAIMQPDNLGTFEIYCQ AGSHREAGMRAIYNVSQCPGHQATPRQRYQAARIYYIMAEEVEWDYCPDRSWEREWHNQSEKDSYGYIFL SNKDGLLGSRYKKAVFREYTDGTFRIPRPRTGPEEHLGILGPLIKGEVGDILTVVFKNNASRPYSVHAHG VLESTTVWPLAAEPGEVVTYQWNIPERSGPGPNDSACVSWIYYSAVDPIKDMYSGLVGPLAICQKGILEP HGGRSDMDREFALLFLIFDENKSWYLEENVATHGSQDPGSINLQDETFLESNKMHAINGKLYANLRGLTM YQGERVAWYMLAMGQDVDLHTIHFHAESFLYRNGENYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTD HVHAGMETLFTVFSRTEHLSPLTVITKETEKAVPPRDIEEGNVKMLGMQIPIKNVEMLASVLVAISVTLL LVVLALGGVVWYQHRQRKLRRNRRSILDDSFKLLSFKQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014799 |
| ORF Size | 3477 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq Size | 4284 bp |
| RefSeq ORF | 2676 bp |
| Locus ID | 9843 |
| UniProt ID | Q9BQS7 |
| Domains | Cu-oxidase |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 130.4 kDa |
| Gene Summary | This gene encodes a member of the multicopper oxidase protein family. The encoded protein is involved in the transport of dietary iron from epithelial cells of the intestinal lumen into the circulatory system, and may be involved in copper transport and homeostasis. In mouse, defects in this gene can lead to severe microcytic anemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204175L3 | Lenti ORF clone of Human hephaestin (HEPH), transcript variant 2, Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC204175L4 | Lenti ORF clone of Human hephaestin (HEPH), transcript variant 2, mGFP tagged |
CNY 9,030.00 |
|
| RG204175 | HEPH (tGFP-tagged) - Human hephaestin (HEPH), transcript variant 2 |
CNY 7,890.00 |
|
| SC110099 | HEPH (untagged)-Human hephaestin (HEPH), transcript variant 2 |
CNY 6,536.00 |
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