DNAJC7 (NM_003315) Human Tagged ORF Clone

CAT#: RC204154

DNAJC7 (Myc-DDK-tagged)-Human DnaJ (Hsp40) homolog, subfamily C, member 7 (DNAJC7), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_003315" in other vectors (6)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Anti-DNAJC7 Rabbit Polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms DJ11; DJC7; TPR2; TTC2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC204154 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCTGCCGCGGAGTGCGATGTGGTAATGGCGGCGACCGAGCCGGAGCTGCTCGACGACCAAGAGG
CGAAGAGGGAAGCAGAGACTTTCAAGGAACAAGGAAATGCATACTATGCCAAGAAAGATTACAATGAAGC
TTATAATTATTATACAAAAGCCATAGATATGTGTCCTAAAAATGCTAGCTATTATGGTAATCGAGCAGCC
ACCTTGATGATGCTTGGAAGGTTCCGGGAAGCTCTTGGAGATGCACAACAGTCAGTGAGGTTGGATGACA
GTTTTGTCCGGGGACATCTACGAGAGGGCAAGTGCCACCTCTCTCTGGGGAATGCCATGGCAGCATGTCG
CAGCTTCCAGAGAGCCCTAGAACTGGATCATAAAAATGCTCAGGCACAACAAGAGTTCAAGAATGCTAAT
GCAGTCATGGAATATGAGAAAATAGCAGAAACAGATTTTGAGAAGCGAGATTTTCGGAAGGTTGTTTTCT
GCATGGACCGTGCCCTAGAATTTGCCCCTGCCTGCCATCGCTTCAAAATCCTCAAGGCAGAATGTTTAGC
AATGCTGGGTCGTTATCCAGAAGCACAGTCTGTGGCTAGTGACATTCTACGAATGGATTCCACCAATGCA
GATGCTCTGTATGTACGAGGTCTTTGCCTTTATTACGAAGATTGTATTGAGAAGGCAGTTCAGTTTTTCG
TACAGGCTCTCAGGATGGCTCCTGACCACGAGAAGGCCTGCATTGCCTGCAGAAATGCCAAAGCACTCAA
AGCAAAGAAAGAAGATGGGAATAAAGCATTTAAGGAAGGAAATTACAAACTAGCATATGAACTGTACACA
GAAGCCCTGGGGATAGACCCCAACAATATAAAAACAAATGCTAAACTCTACTGTAATCGGGGTACGGTTA
ATTCCAAGCTTAGGAAACTAGATGATGCAATAGAAGACTGCACAAATGCAGTGAAGCTTGATGACACTTA
CATAAAAGCCTACTTGAGAAGAGCTCAGTGTTACATGGACACAGAACAGTATGAAGAAGCAGTACGAGAC
TATGAAAAAGTATACCAGACAGAGAAAACAAAAGAACACAAACAGCTCCTAAAAAATGCGCAGCTGGAAC
TGAAGAAGAGTAAGAGGAAAGATTACTACAAGATTCTAGGAGTGGACAAGAATGCCTCTGAGGACGAGAT
CAAGAAAGCTTATCGGAAACGGGCCTTGATGCACCATCCAGATCGGCATAGTGGAGCCAGTGCTGAGGTT
CAGAAGGAGGAGGAGAAGAAGTTCAAGGAAGTTGGAGAGGCCTTTACTATCCTCTCTGATCCCAAGAAAA
AGACTCGCTATGACAGTGGACAGGACCTAGATGAGGAGGGCATGAATATGGGTGATTTTGATCCAAACAA
TATCTTCAAGGCATTCTTTGGCGGTCCTGGCGGCTTCAGCTTTGAAGCATCTGGTCCAGGGAATTTCTTT
TTTCAATTTGGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC204154 protein sequence
Red=Cloning site Green=Tags(s)

MAAAAECDVVMAATEPELLDDQEAKREAETFKEQGNAYYAKKDYNEAYNYYTKAIDMCPKNASYYGNRAA
TLMMLGRFREALGDAQQSVRLDDSFVRGHLREGKCHLSLGNAMAACRSFQRALELDHKNAQAQQEFKNAN
AVMEYEKIAETDFEKRDFRKVVFCMDRALEFAPACHRFKILKAECLAMLGRYPEAQSVASDILRMDSTNA
DALYVRGLCLYYEDCIEKAVQFFVQALRMAPDHEKACIACRNAKALKAKKEDGNKAFKEGNYKLAYELYT
EALGIDPNNIKTNAKLYCNRGTVNSKLRKLDDAIEDCTNAVKLDDTYIKAYLRRAQCYMDTEQYEEAVRD
YEKVYQTEKTKEHKQLLKNAQLELKKSKRKDYYKILGVDKNASEDEIKKAYRKRALMHHPDRHSGASAEV
QKEEEKKFKEVGEAFTILSDPKKKTRYDSGQDLDEEGMNMGDFDPNNIFKAFFGGPGGFSFEASGPGNFF
FQFG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003315
ORF Size 1485 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_003315.4
RefSeq Size 2096 bp
RefSeq ORF 1485 bp
Locus ID 7266
UniProt ID Q99615
Domains TPR, DnaJ
MW 56.4 kDa
Gene Summary This gene encodes a member of the DNAJ heat shock protein 40 family of proteins that is characterized by two N-terminal tetratricopeptide repeat domains and a C-terminal DNAJ domain. This protein binds the chaperone proteins heat shock proteins 70 and 90 in an ATP-dependent manner and may function as a co-chaperone. Pseudogenes of this gene are found on chromosomes 1 and 6. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2009]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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