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COQ4 (NM_016035) Human Tagged ORF Clone
CAT＃: RC204104
COQ4 (Myc-DDK-tagged)-Human coenzyme Q4 homolog (S. cerevisiae) (COQ4), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_016035" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CGI-92; COQ10D7 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204104 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACTCTGCTGCGCCCTGTCCTCCGTCGGCTCTGCGGGCTCCCGGGCCTACAGCGGCCTGCGGCAG AAATGCCCCTCCGGGCTAGGAGCGACGGCGCCGGCCCGCTATACTCGCACCACCTCCCCACCTCCCCGCT GCAGAAAGCGCTGTTGGCCGCCGGCTCCGCGGCGATGGCGCTCTATAACCCCTACCGCCACGACATGGTC GCAGTTCTAGGGGAGACCACAGGACACCGCACCCTGAAGGTCCTCAGGGACCAGATGAGGAGGGATCCAG AGGGTGCCCAGATCCTGCAGGAGCGTCCCCGGATTTCGACATCCACCCTCGACCTGGGCAAGCTCCAGAG CCTGCCGGAAGGCTCCCTCGGTCGCGAGTATCTCCGTTTCCTGGATGTGAACAGGGTCTCCCCAGACACC CGAGCACCCACCCGCTTCGTGGATGATGAGGAGCTAGCGTATGTGATTCAGCGGTACCGGGAGGTGCACG ACATGCTTCACACCCTGCTGGGGATGCCCACCAACATCCTGGGGGAGATCGTGGTGAAATGGTTTGAGGC TGTCCAGACTGGCCTGCCCATGTGCATCCTGGGTGCATTCTTTGGACCGATCCGACTTGGCGCTCAGAGC CTGCAAGTGCTGGTCTCGGAGTTGATCCCATGGGCCGTTCAGAACGGGCGCAGAGCCCCATGTGTCCTCA ACCTGTACTATGAGCGGCGCTGGGAGCAGTCCCTGAGGGCTCTGCGGGAGGAGCTGGGCATTACAGCACC ACCCATGCACGTCCAGGGCTTGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204104 protein sequence
Red=Cloning site Green=Tags(s) MATLLRPVLRRLCGLPGLQRPAAEMPLRARSDGAGPLYSHHLPTSPLQKALLAAGSAAMALYNPYRHDMV AVLGETTGHRTLKVLRDQMRRDPEGAQILQERPRISTSTLDLGKLQSLPEGSLGREYLRFLDVNRVSPDT RAPTRFVDDEELAYVIQRYREVHDMLHTLLGMPTNILGEIVVKWFEAVQTGLPMCILGAFFGPIRLGAQS LQVLVSELIPWAVQNGRRAPCVLNLYYERRWEQSLRALREELGITAPPMHVQGLA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016035 |
| ORF Size | 795 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016035.4 |
| RefSeq Size | 1597 bp |
| RefSeq ORF | 798 bp |
| Locus ID | 51117 |
| UniProt ID | Q9Y3A0 |
| Domains | Coq4 |
| MW | 29.7 kDa |
| Gene Summary | This gene encodes a component of the coenzyme Q biosynthesis pathway. Coenzyme Q, an essential component of the electron transport chain, shuttles electrons between complexes I or II to complex III of the mitochondrial transport chain. This protein appears to play a structural role in stabilizing a complex that contains most of the coenzyme Q biosynthesis enzymes. Mutations in this gene are associated with mitochondrial disorders linked to coenzyme Q deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204104L3 | Lenti ORF clone of Human coenzyme Q4 homolog (S. cerevisiae) (COQ4), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204104L4 | Lenti ORF clone of Human coenzyme Q4 homolog (S. cerevisiae) (COQ4), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 4,800.00 |
|
| RG204104 | COQ4 (tGFP-tagged) - Human coenzyme Q4 homolog (S. cerevisiae) (COQ4), nuclear gene encoding mitochondrial protein |
CNY 4,000.00 |
|
| SC319900 | COQ4 (untagged)-Human coenzyme Q4 homolog (S. cerevisiae) (COQ4), nuclear gene encoding mitochondrial protein |
CNY 2,400.00 |
