COQ4 (NM_016035) Human Tagged ORF Clone

CAT#: RG204104

  • TrueORF®

COQ4 (tGFP-tagged) - Human coenzyme Q4 homolog (S. cerevisiae) (COQ4), nuclear gene encoding mitochondrial protein

ORF Plasmid: DDK tGFP



  "NM_016035" in other vectors (4)

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CNY 4,000.00

CNY 4,370.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms CGI-92; COQ10D7
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG204104 representing NM_016035
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGACTCTGCTGCGCCCTGTCCTCCGTCGGCTCTGCGGGCTCCCGGGCCTACAGCGGCCTGCGGCAG
AAATGCCCCTCCGGGCTAGGAGCGACGGCGCCGGCCCGCTATACTCGCACCACCTCCCCACCTCCCCGCT
GCAGAAAGCGCTGTTGGCCGCCGGCTCCGCGGCGATGGCGCTCTATAACCCCTACCGCCACGACATGGTC
GCAGTTCTAGGGGAGACCACAGGACACCGCACCCTGAAGGTCCTCAGGGACCAGATGAGGAGGGATCCAG
AGGGTGCCCAGATCCTGCAGGAGCGTCCCCGGATTTCGACATCCACCCTCGACCTGGGCAAGCTCCAGAG
CCTGCCGGAAGGCTCCCTCGGTCGCGAGTATCTCCGTTTCCTGGATGTGAACAGGGTCTCCCCAGACACC
CGAGCACCCACCCGCTTCGTGGATGATGAGGAGCTAGCGTATGTGATTCAGCGGTACCGGGAGGTGCACG
ACATGCTTCACACCCTGCTGGGGATGCCCACCAACATCCTGGGGGAGATCGTGGTGAAATGGTTTGAGGC
TGTCCAGACTGGCCTGCCCATGTGCATCCTGGGTGCATTCTTTGGACCGATCCGACTTGGCGCTCAGAGC
CTGCAAGTGCTGGTCTCGGAGTTGATCCCATGGGCCGTTCAGAACGGGCGCAGAGCCCCATGTGTCCTCA
ACCTGTACTATGAGCGGCGCTGGGAGCAGTCCCTGAGGGCTCTGCGGGAGGAGCTGGGCATTACAGCACC
ACCCATGCACGTCCAGGGCTTGGCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG204104 representing NM_016035
Red=Cloning site Green=Tags(s)

MATLLRPVLRRLCGLPGLQRPAAEMPLRARSDGAGPLYSHHLPTSPLQKALLAAGSAAMALYNPYRHDMV
AVLGETTGHRTLKVLRDQMRRDPEGAQILQERPRISTSTLDLGKLQSLPEGSLGREYLRFLDVNRVSPDT
RAPTRFVDDEELAYVIQRYREVHDMLHTLLGMPTNILGEIVVKWFEAVQTGLPMCILGAFFGPIRLGAQS
LQVLVSELIPWAVQNGRRAPCVLNLYYERRWEQSLRALREELGITAPPMHVQGLA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_016035
ORF Size 795 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_016035.4
RefSeq Size 1548 bp
RefSeq ORF 798 bp
Locus ID 51117
UniProt ID Q9Y3A0
Domains Coq4
Gene Summary This gene encodes a component of the coenzyme Q biosynthesis pathway. Coenzyme Q, an essential component of the electron transport chain, shuttles electrons between complexes I or II to complex III of the mitochondrial transport chain. This protein appears to play a structural role in stabilizing a complex that contains most of the coenzyme Q biosynthesis enzymes. Mutations in this gene are associated with mitochondrial disorders linked to coenzyme Q deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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