MAGED1 (NM_001005333) Human Tagged ORF Clone
CAT#: RC204088
MAGED1 (Myc-DDK-tagged)-Human melanoma antigen family D, 1 (MAGED1), transcript variant 1
ORF Plasmid: tGFP
"NM_001005333" in other vectors (5)
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CNY 6,112.00
CNY 7,130.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | DLXIN-1; NRAGE |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC204088 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCAGAAAATGGACTGTGGTGCGGGCCTCCTCGGCTTCCAGAATCCTGACGCTTGTAGAGCAGTCT GTCACCCCCTCCCCCAGCCTCCTGCCTCCACCCTCCCTCTTTCTGCATTCCCCACTCTGTGCGACCCCCC TTATTCTCAACTCCGCGATCCGCCTGCCGTCCTGAGCTGCTACTGCACGCCTCTTGGAGCGTCCCCGGCT CCTGCTGAGGCCTCCGTAGAAGACAGCGCCTTGCTTATGCAGACCTTGATGGAGGCCATCCAGATCTCAG AGGCTCCACCTACTAACCAGGCCACCGCAGCTGCTAGTCCCCAGAGTTCACAGCCCCCAACTGCCAATGA GATGGCTGACATTCAGGTTTCAGCAGCTGCCGCTAGGCCTAAGTCAGCCTTTAAAGTCCAGAATGCCACC ACAAAAGGCCCAAATGGTGTCTATGATTTCTCTCAGGCTCATAATGCCAAGGATGTGCCCAACACGCAGC CCAAGGCAGCCTTTAAGTCCCAAAATGCTACCCCAAAGGGTCCAAATGCTGCCTATGATTTTTCCCAGGC AGCAACCACTGGTGAGTTAGCTGCTAACAAGTCTGAGATGGCCTTCAAGGCCCAGAATGCCACTACTAAA GTGGGCCCAAATGCCACCTACAATTTCTCTCAGTCTCTCAATGCCAATGACCTGGCCAACAGCAGGCCTA AGACCCCTTTCAAGGCTTGGAATGATACCACTAAGGCCCCAACAGCTGATACCCAGACCCAGAATGTAAA TCAGGCCAAAATGGCCACTTCCCAGGCTGACATAGAGACCGACCCAGGTATCTCTGAACCTGACGGTGCA ACTGCACAGACATCAGCAGATGGTTCCCAGGCTCAGAATCTGGAGTCCCGGACAATAATTCGGGGCAAGA GGACCCGCAAGATTAATAACTTGAATGTTGAAGAGAACAGCAGTGGGGATCAGAGGCGGGCCCCACTGGC TGCAGGGACCTGGAGGTCTGCACCAGTTCCAGTGACCACTCAGAACCCACCTGGCGCACCCCCCAATGTG CTCTGGCAGACGCCATTGGCTTGGCAGAACCCCTCAGGCTGGCAAAACCAGACAGCCAGGCAGACCCCAC CAGCACGTCAGAGCCCTCCAGCTAGGCAGACCCCACCAGCCTGGCAGAACCCAGTCGCTTGGCAGAACCC AGTGATTTGGCCAAACCCAGTAATCTGGCAGAACCCAGTGATCTGGCCAAACCCCATTGTCTGGCCCGGC CCTGTTGTCTGGCCGAATCCACTGGCCTGGCAGAATCCACCTGGATGGCAGACTCCACCTGGATGGCAGA CCCCACCGGGCTGGCAGGGTCCTCCAGACTGGCAAGGTCCTCCTGACTGGCCGCTACCACCCGACTGGCC ACTGCCACCTGATTGGCCACTTCCCACTGACTGGCCACTACCACCTGACTGGATCCCCGCTGATTGGCCA ATTCCACCTGACTGGCAGAACCTGCGCCCCTCGCCTAACCTGCGCCCTTCTCCCAACTCGCGTGCCTCAC AGAACCCAGGTGCTGCACAGCCCCGAGATGTGGCCCTTCTTCAGGAAAGAGCAAATAAGTTGGTCAAGTA CTTGATGCTTAAGGACTACACAAAGGTGCCCATCAAGCGCTCAGAAATGCTGAGAGATATCATCCGTGAA TACACTGATGTTTATCCAGAAATCATTGAACGTGCATGCTTTGTCCTAGAGAAGAAATTTGGGATTCAAC TGAAAGAAATTGACAAAGAAGAACACCTGTATATTCTCATCAGTACCCCCGAGTCCCTGGCTGGCATACT GGGAACGACCAAAGACACACCCAAGCTCGGTCTCCTCTTGGTGATTCTGGGTGTCATCTTCATGAATGGC AACCGTGCCAGTGAGGCTGTCCTCTGGGAGGCACTACGCAAGATGGGACTGCGTCCTGGGGTGAGACATC CCCTCCTTGGAGATCTAAGGAAACTTCTCACCTATGAGTTTGTAAAGCAGAAATACCTGGACTACAGACG AGTGCCCAACAGCAACCCCCCGGAGTATGAGTTCCTCTGGGGCCTCCGTTCCTACCATGAGACTAGCAAG ATGAAAGTGCTGAGATTCATTGCAGAGGTTCAGAAAAGAGACCCTCGTGACTGGACTGCACAGTTCATGG AGGCTGCAGATGAGGCCTTGGATGCTCTGGATGCTGCTGCAGCTGAGGCCGAAGCCCGGGCTGAAGCAAG AACCCGCATGGGAATTGGAGATGAGGCTGTGTCTGGGCCCTGGAGCTGGGATGACATTGAGTTTGAGCTG CTGACCTGGGATGAGGAAGGAGATTTTGGAGATCCCTGGTCCAGAATTCCATTTACCTTCTGGGCCAGAT ACCACCAGAATGCCCGCTCCAGATTCCCTCAGACCTTTGCCGGTCCCATTATTGGTCCTGGTGGTACAGC CAGTGCCAACTTCGCTGCCAACTTTGGTGCCATTGGTTTCTTCTGGGTTGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204088 protein sequence
Red=Cloning site Green=Tags(s) MAQKMDCGAGLLGFQNPDACRAVCHPLPQPPASTLPLSAFPTLCDPPYSQLRDPPAVLSCYCTPLGASPA PAEASVEDSALLMQTLMEAIQISEAPPTNQATAAASPQSSQPPTANEMADIQVSAAAARPKSAFKVQNAT TKGPNGVYDFSQAHNAKDVPNTQPKAAFKSQNATPKGPNAAYDFSQAATTGELAANKSEMAFKAQNATTK VGPNATYNFSQSLNANDLANSRPKTPFKAWNDTTKAPTADTQTQNVNQAKMATSQADIETDPGISEPDGA TAQTSADGSQAQNLESRTIIRGKRTRKINNLNVEENSSGDQRRAPLAAGTWRSAPVPVTTQNPPGAPPNV LWQTPLAWQNPSGWQNQTARQTPPARQSPPARQTPPAWQNPVAWQNPVIWPNPVIWQNPVIWPNPIVWPG PVVWPNPLAWQNPPGWQTPPGWQTPPGWQGPPDWQGPPDWPLPPDWPLPPDWPLPTDWPLPPDWIPADWP IPPDWQNLRPSPNLRPSPNSRASQNPGAAQPRDVALLQERANKLVKYLMLKDYTKVPIKRSEMLRDIIRE YTDVYPEIIERACFVLEKKFGIQLKEIDKEEHLYILISTPESLAGILGTTKDTPKLGLLLVILGVIFMNG NRASEAVLWEALRKMGLRPGVRHPLLGDLRKLLTYEFVKQKYLDYRRVPNSNPPEYEFLWGLRSYHETSK MKVLRFIAEVQKRDPRDWTAQFMEAADEALDALDAAAAEAEARAEARTRMGIGDEAVSGPWSWDDIEFEL LTWDEEGDFGDPWSRIPFTFWARYHQNARSRFPQTFAGPIIGPGGTASANFAANFGAIGFFWVE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001005333 |
ORF Size | 2502 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001005333.2 |
RefSeq Size | 2928 bp |
RefSeq ORF | 2505 bp |
Locus ID | 9500 |
UniProt ID | Q9Y5V3 |
Protein Families | Druggable Genome |
Protein Pathways | Neurotrophin signaling pathway |
MW | 92 kDa |
Gene Summary | This gene is a member of the melanoma antigen gene (MAGE) family. Most of the genes of this family encode tumor specific antigens that are not expressed in normal adult tissues except testis. Although the protein encoded by this gene shares strong homology with members of the MAGE family, it is expressed in almost all normal adult tissues. This gene has been demonstrated to be involved in the p75 neurotrophin receptor mediated programmed cell death pathway. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC204088L3 | Lenti ORF clone of Human melanoma antigen family D, 1 (MAGED1), transcript variant 1, Myc-DDK-tagged |
CNY 9,030.00 |
|
RC204088L4 | Lenti ORF clone of Human melanoma antigen family D, 1 (MAGED1), transcript variant 1, mGFP tagged |
CNY 9,030.00 |
|
RG204088 | MAGED1 (tGFP-tagged) - Human melanoma antigen family D, 1 (MAGED1), transcript variant 1 |
CNY 7,890.00 |
|
SC300906 | MAGED1 (untagged)-Human melanoma antigen family D, 1 (MAGED1), transcript variant 1 |
CNY 7,220.00 |
|
SC320487 | MAGED1 (untagged)-Human melanoma antigen family D, 1 (MAGED1), transcript variant 1 |
CNY 6,120.00 |