AIPL1 (NM_014336) Human Tagged ORF Clone
CAT#: RC204079
AIPL1 (Myc-DDK-tagged)-Human aryl hydrocarbon receptor interacting protein-like 1 (AIPL1), transcript variant 1
ORF Plasmid: tGFP
"NM_014336" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AIPL2; LCA4 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC204079 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGCCGCTCTGCTCCTGAACGTGGAAGGGGTCAAGAAAACCATTCTGCACGGGGGCACGGGCGAGC TCCCAAACTTCATCACCGGATCCCGAGTGATCTTTCATTTCCGCACCATGAAATGTGATGAGGAGCGGAC AGTCATTGACGACAGTCGGCAGGTGGGCCAGCCCATGCACATCATCATCGGAAACATGTTCAAGCTCGAG GTCTGGGAGATCCTGCTTACCTCCATGCGGGTGCACGAGGTGGCCGAGTTCTGGTGCGACACCATCCACA CGGGGGTCTACCCCATCCTGTCCCGGAGCCTGAGGCAGATGGCCCAGGGCAAGGACCCCACAGAGTGGCA CGTGCACACGTGCGGGCTGGCCAACATGTTCGCCTACCACACGCTGGGCTACGAGGACCTGGACGAGCTG CAGAAGGAGCCTCAGCCTCTGGTCTTTGTGATCGAGCTGCTGCAGGTTGATGCCCCGAGTGATTACCAGA GGGAGACCTGGAACCTGAGCAATCATGAGAAGATGAAGGCGGTGCCCGTCCTCCACGGAGAGGGAAATCG GCTCTTCAAGCTGGGCCGCTACGAGGAGGCCTCTTCCAAGTACCAGGAGGCCATCATCTGCCTAAGGAAC CTGCAGACCAAGGAGAAGCCGTGGGAGGTGCAGTGGCTGAAGCTGGAGAAGATGATCAATACTCTGATCC TCAACTACTGCCAGTGCCTGCTGAAGAAGGAGGAGTACTATGAGGTGCTGGAGCACACCAGTGATATTCT CCGGCACCACCCAGGCATCGTGAAGGCCTACTACGTGCGTGCCCGGGCTCACGCAGAGGTGTGGAATGAG GCCGAGGCCAAGGCGGACCTCCAGAAAGTGCTGGAGCTGGAGCCGTCCATGCAGAAGGCGGTGCGCAGGG AGCTGAGGCTGCTGGAGAACCGCATGGCGGAGAAGCAGGAGGAGGAGCGGCTGCGCTGCCGGAACATGCT GAGCCAGGGTGCCACGCAGCCTCCCGCAGAGCCACCCACAGAGCCACCCGCACAGTCATCCACAGAGCCA CCTGCAGAGCCACCCACAGCACCATCTGCAGAGCTGTCCGCAGGGCCCCCTGCAGAGCCAGCCACAGAGC CACCCCCGTCCCCAGGGCACTCGCTGCAGCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204079 protein sequence
Red=Cloning site Green=Tags(s) MDAALLLNVEGVKKTILHGGTGELPNFITGSRVIFHFRTMKCDEERTVIDDSRQVGQPMHIIIGNMFKLE VWEILLTSMRVHEVAEFWCDTIHTGVYPILSRSLRQMAQGKDPTEWHVHTCGLANMFAYHTLGYEDLDEL QKEPQPLVFVIELLQVDAPSDYQRETWNLSNHEKMKAVPVLHGEGNRLFKLGRYEEASSKYQEAIICLRN LQTKEKPWEVQWLKLEKMINTLILNYCQCLLKKEEYYEVLEHTSDILRHHPGIVKAYYVRARAHAEVWNE AEAKADLQKVLELEPSMQKAVRRELRLLENRMAEKQEEERLRCRNMLSQGATQPPAEPPTEPPAQSSTEP PAEPPTAPSAELSAGPPAEPATEPPPSPGHSLQH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_014336 |
ORF Size | 1152 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_014336.5 |
RefSeq Size | 2990 bp |
RefSeq ORF | 1155 bp |
Locus ID | 23746 |
UniProt ID | Q9NZN9 |
Protein Families | Druggable Genome |
MW | 43.9 kDa |
Gene Summary | Leber congenital amaurosis (LCA) is the most severe inherited retinopathy with the earliest age of onset and accounts for at least 5% of all inherited retinal diseases. Affected individuals are diagnosed at birth or in the first few months of life with nystagmus, severely impaired vision or blindness and an abnormal or flat electroretinogram. The photoreceptor/pineal-expressed gene, AIPL1, encoding aryl-hydrocarbon interacting protein-like 1, is located within the LCA4 candidate region. The encoded protein contains three tetratricopeptide motifs, consistent with chaperone or nuclear transport activity. Mutations in this gene may cause approximately 20% of recessive LCA. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC204079L3 | Lenti ORF clone of Human aryl hydrocarbon receptor interacting protein-like 1 (AIPL1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC204079L4 | Lenti ORF clone of Human aryl hydrocarbon receptor interacting protein-like 1 (AIPL1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RG204079 | AIPL1 (tGFP-tagged) - Human aryl hydrocarbon receptor interacting protein-like 1 (AIPL1), transcript variant 1 |
CNY 5,256.00 |
|
SC321437 | AIPL1 (untagged)-Human aryl hydrocarbon receptor interacting protein-like 1 (AIPL1), transcript variant 1 |
CNY 3,656.00 |