United States
Germany
Japan
United Kingdom
China
TLE 1 (TLE1) (NM_005077) Human Tagged ORF Clone
CAT＃: RC204037
- TrueORF®
TLE1 (Myc-DDK-tagged)-Human transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila) (TLE1)
ORF Plasmid: tGFP
"NM_005077" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 8,456.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ESG; ESG1; GRG1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204037 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCCGCAGAGCCGGCACCCGACGCCGCACCAGGCTGCAGGCCAGCCCTTCAAGTTCACTATCCCGG AGTCCCTGGACCGGATTAAAGAGGAATTCCAGTTCCTGCAGGCGCAGTATCACAGCCTTAAATTGGAATG TGAGAAACTGGCAAGTGAAAAGACAGAAATGCAGAGGCACTATGTGATGTATTATGAAATGTCATATGGA TTAAACATTGAAATGCACAAACAGACTGAAATCGCCAAGAGATTGAATACGATTTGTGCACAAGTCATCC CATTTCTGTCTCAGGAACATCAACAACAGGTGGCCCAGGCTGTTGAACGTGCCAAACAGGTGACCATGGC AGAGTTGAATGCCATCATCGGGCAGCAGCAGTTGCAAGCTCAGCATCTTTCTCATGGCCACGGACCCCCA GTTCCCCTTACGCCTCACCCTTCGGGACTTCAGCCTCCTGGAATCCCGCCCCTCGGGGGCAGTGCCGGCC TTCTTGCGCTGTCTAGTGCTCTGAGTGGGCAGTCTCACTTGGCAATAAAAGATGACAAGAAGCACCACGA TGCAGAGCACCACAGAGACAGAGAGCCGGGCACAAGTAATTCCCTCCTGGTCCCAGACAGTCTAAGAGGC ACAGATAAACGCAGAAATGGACCTGAATTTTCCAATGACATCAAGAAAAGGAAGGTGGATGATAAGGACT CCAGCCACTATGACAGTGATGGTGACAAAAGCGATGACAACTTAGTTGTGGATGTGTCTAATGAGGACCC TTCTTCTCCGCGAGCAAGCCCTGCCCACTCGCCCCGGGAAAATGGAATCGACAAAAATCGCCTGCTAAAG AAGGATGCTTCTAGCAGTCCAGCTTCCACGGCCTCCTCGGCAAGTTCCACTTCTTTGAAATCCAAAGAAA TGAGCTTGCATGAAAAAGCCAGCACGCCTGTTCTGAAATCCAGCACACCAACGCCTCGGAGCGACATGCC AACGCCGGGCACCAGCGCCACTCCAGGCCTCCGTCCAGGTCTCGGCAAGCCTCCAGCCATAGACCCCCTC GTTAACCAAGCGGCAGCTGGCTTGAGGACACCCCTGGCAGTGCCCGGCCCATATCCTGCTCCTTTTGGGA TGGTCCCCCACGCTGGCATGAACGGCGAGCTGACCAGCCCAGGCGCTGCCTACGCCAGTTTACACAACAT GTCGCCCCAGATGAGCGCCGCAGCCGCCGCGGCCGCCGTGGTGGCCTACGGGCGCTCCCCCATGGTGGGG TTTGATCCTCCCCCTCACATGAGAGTACCTACCATTCCTCCAAACCTGGCAGGAATCCCTGGGGGGAAAC CTGCATACTCCTTCCACGTTACTGCAGACGGTCAGATGCAGCCTGTCCCTTTTCCCCCCGACGCCCTCAT CGGACCCGGAATCCCCCGGCATGCTCGCCAGATCAACACCCTCAACCACGGGGAGGTGGTGTGCGCTGTG ACCATCAGCAACCCCACGAGACACGTGTACACAGGCGGGAAGGGCTGCGTCAAGGTCTGGGACATCAGCC ACCCTGGCAATAAGAGCCCTGTCTCCCAGCTCGACTGTCTGAACAGAGACAATTATATCCGTTCCTGTAA ATTGCTACCCGATGGCTGCACTCTCATAGTGGGAGGGGAAGCCAGTACTTTGTCCATTTGGGACCTGGCG GCTCCAACCCCGCGCATCAAGGCGGAGCTGACGTCCTCGGCCCCCGCCTGCTACGCCCTGGCCATCAGCC CCGATTCCAAGGTCTGCTTCTCATGCTGCAGCGACGGCAACATCGCTGTGTGGGATCTGCACAACCAGAC ACTAGTGAGGCAATTCCAGGGCCACACAGACGGAGCCAGCTGTATTGACATTTCTAATGATGGCACCAAG CTCTGGACGGGTGGTTTGGACAACACAGTCAGGTCCTGGGACCTGCGCGAGGGGCGGCAGCTGCAGCAGC ACGACTTCACCTCCCAGATCTTCTCCCTGGGGTACTGCCCCACCGGGGAGTGGCTGGCAGTGGGCATGGA GAGCAGCAATGTGGAGGTGCTGCACGTGAACAAGCCTGACAAGTACCAGCTGCACCTGCATGAGAGCTGC GTGCTGTCCCTGAAATTTGCTTACTGTGGTAAATGGTTTGTGAGTACTGGAAAAGATAACCTCCTCAATG CTTGGCGGACCCCCTATGGAGCCAGCATATTCCAGTCCAAAGAGTCCTCGTCAGTGCTTAGCTGTGACAT CTCTGTGGATGATAAGTACATAGTCACTGGCTCGGGGGACAAGAAGGCTACAGTCTATGAAGTCATCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204037 protein sequence
Red=Cloning site Green=Tags(s) MFPQSRHPTPHQAAGQPFKFTIPESLDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYG LNIEMHKQTEIAKRLNTICAQVIPFLSQEHQQQVAQAVERAKQVTMAELNAIIGQQQLQAQHLSHGHGPP VPLTPHPSGLQPPGIPPLGGSAGLLALSSALSGQSHLAIKDDKKHHDAEHHRDREPGTSNSLLVPDSLRG TDKRRNGPEFSNDIKKRKVDDKDSSHYDSDGDKSDDNLVVDVSNEDPSSPRASPAHSPRENGIDKNRLLK KDASSSPASTASSASSTSLKSKEMSLHEKASTPVLKSSTPTPRSDMPTPGTSATPGLRPGLGKPPAIDPL VNQAAAGLRTPLAVPGPYPAPFGMVPHAGMNGELTSPGAAYASLHNMSPQMSAAAAAAAVVAYGRSPMVG FDPPPHMRVPTIPPNLAGIPGGKPAYSFHVTADGQMQPVPFPPDALIGPGIPRHARQINTLNHGEVVCAV TISNPTRHVYTGGKGCVKVWDISHPGNKSPVSQLDCLNRDNYIRSCKLLPDGCTLIVGGEASTLSIWDLA APTPRIKAELTSSAPACYALAISPDSKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISNDGTK LWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHVNKPDKYQLHLHESC VLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISVDDKYIVTGSGDKKATVYEVIY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005077 |
| ORF Size | 2310 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005077.3, NP_005068.2 |
| RefSeq Size | 3283 bp |
| RefSeq ORF | 2313 bp |
| Locus ID | 7088 |
| UniProt ID | Q04724 |
| Domains | WD40, TLE_N |
| Protein Families | Transcription Factors |
| MW | 83.2 kDa |
| Gene Summary | Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
TLE1 Is an Anoikis Regulator and Is Downregulated by Bit1 in Breast Cancer Cells
,Chris Brunquell, Hector Biliran, Scott Jennings, Shubha Kale Ireland, Renwei Chen, and Erkki Ruoslahti,
Mol. Cancer Res., Nov 2012; 10: 1482 - 1495.
[TLE 1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204037L1 | Lenti ORF clone of Human transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila) (TLE1), Myc-DDK-tagged |
CNY 10,856.00 |
|
| RC204037L2 | Lenti ORF clone of Human transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila) (TLE1), mGFP tagged |
CNY 10,856.00 |
|
| RC204037L3 | Lenti ORF clone of Human transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila) (TLE1), Myc-DDK-tagged |
CNY 10,856.00 |
|
| RC204037L4 | Lenti ORF clone of Human transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila) (TLE1), mGFP tagged |
CNY 10,856.00 |
|
| RG204037 | TLE1 (tGFP-tagged) - Human transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila) (TLE1) |
CNY 10,056.00 |
|
| SC310123 | TLE1 (untagged)-Human transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila) (TLE1) |
CNY 5,648.00 |
